Cytofect™ Transfection Kits are plasmid DNA delivery systems specifically optimized to deliver DNA into a wide variety of cell types with high efficiency. Below, selected Cytofect-Transfection Kits were validated by transfecting cells with GFP-expression plasmid. Images show highly efficient uptake of DNA, as evidenced by visualization of green fluorescent protein.
Jump to Transfection Images:
|
|
HPAEC | |
 |
 |
|
| HLMVEC | HDMVEC | |
 |
 |
|
| HDLMVEC | PPAEC | |
|
 |
|
| BAOEC | RAOEC | |
 |
 |
|
| HCAEC | RBMVEC | |
 |
| HCF | HDF | |
 |
 |
|
| HLF | ||
 |
| HAOSMC | HCASMC | |
 |
 |
|
| HPASMC | HITASMC | |
 |
 |
 |
|
293
|
|
CHO
|
 |
|
 |
|
|
||
|
HeLa
|
|
MCF7
|
 |
 |
Examples of High Transfection Efficiency using Cytofect™ Kits:
| Cytofect™ | Cell Type |
Efficiency | Viability |
| Cell Line | Human Breast Adenocarcinoma (MCF-7) | 40-90% | 80-95% |
| Human Cervical Epithelial Adenocarcinoma (HeLa) | |||
| Human Embryonic Kidney (293) | |||
| Chinese Hamster Ovary (CHO) | |||
| Chondrocyte | Human Chondrocytes (HC) | 35% | 80-95% |
| Endothelial | Human Aortic Endothelial Cells (HAOEC) | 40-85% | 80-95% |
| Human Coronary Artery Endothelial Cells (HCAEC) | |||
| Human Dermal Lymphatic Microvascular Endothelial Cells (HDLMVEC) | |||
| Human Dermal Microvascular Endothelial Cells (HDMVEC) | |||
| Human Lung Microvascular Endothelial Cells (HLMVEC) | |||
| Human Pulmonary Artery Endothelial Cells (HPAEC) | |||
| Human Umbilical Vein Endothelial Cells (HUVEC) | |||
| Bovine Aortic Endothelial Cells (BAOEC) | |||
| Bovine Coronary Artery Endothelial Cells (BCAEC) | |||
| Bovine Pulmonary Artery Endothelial Cells (BPAEC) | |||
| Porcine Pulmonary Artery Endothelial Cells (PPAEC) | |||
| Rat Aortic Endothelial Cells (RAOEC) | |||
| Rat Brain Microvascular Endothelial Cells (RBMVEC) | |||
| Epithelial | Human Bronchial Epithelial Cells (HBEpC) | 40-70% | 70-95% |
| Human Epidermal Keratinocytes (HEK) | |||
| Human Mammary Epithelial Cells (HMEpC) | |||
| Human Prostate Epithelial Cells (HPrEpC) | |||
| Human Tracheal Epithelial Cells (HTEpC) | |||
| Human Umbilical Epithelial Cells | |||
| Fibroblast | Human Cardiac Fibroblasts (HCF) | 30-70% | 90-95% |
| Human Dermal Fibroblasts (HDF) | |||
| Human Lung Fibroblasts (HLF) | |||
| Hepatocyte | Rat Hepatocytes (RH) | 40-60% | 70-95% |
| HUVEC | Human Umbilical Vein Endothelial Cells (HUVEC) | 40-85% | 80-95% |
| Neuron | Rat Cortical Neurons (RCoN) | 10-20% | 80-95% |
| Preadipocyte | Human Preadipocytes (HPAd) | 25% | 80-95% |
| Skeletal Muscle | Human Skeletal Muscle Cells (HSkMC) | 15-30% | 80-95% |
| Smooth Muscle | Human Aortic Smooth Muscle Cells (HAOSMC) | 35-65% | 80-95% |
| Human Brachiocephalic Artery Smooth Muscle Cells (HBcASMC) | |||
| Human Carotid Artery Smooth Muscle Cells (HCtASMC) | |||
| Human Coronary Artery Smooth Muscle Cells (HCASMC) | |||
| Human Internal Thoracic Artery Smooth Muscle Cells (HITASMC) | |||
| Human Pulmonary Artery Smooth Muscle Cells (HPASMC) | |||
| Human Subclavian Artery Smooth Muscle Cells (HScASMC) | |||
| Human Umbilical Vein Smooth Muscle Cells (HUVSMC) |