Cytofect™ Transfection Reagents are exceptionally gentle, and have minimal effects on cellular morphology as seen in these post-transfection images of various primary cells and cell lines. Post-transfection cell viability is a key parameter of transfection system performance. The Cytofect™ Transfection Reagents provide exceptionally high post-transfection viabilities compared to both electroporation and other chemical based transfection reagents. Even after passaging, cells transfected with Cytofect™ continue to express transgenic protein as seen below. 
 
Image: Cytofect™ Transfection Kits maintain cell morphology & transgene expression even after subculturing.
Here, Human Umbilical Vein Endothelial Cells (HUVEC) are transfected with a GFP expression plasmid.
 
 
Examples of High Post-Transfection Cell Health, Viability and Morphology
 
 
 
 
 

Examples of High Cell Viability (Low Toxicity) Post-Transfection using Cytofect™ Transfection Kits:

Cytofect™ Cell Type
Efficiency  Viability
 
     
Cell Line Human Breast Adenocarcinoma (MCF-7)  40-90%  80-95%
  Human Cervical Epithelial Adenocarcinoma (HeLa)    
  Human Embryonic Kidney (293)    
  Chinese Hamster Ovary (CHO)    
       
Chondrocyte Human Chondrocytes (HC) 35%  80-95%
       
Endothelial Human Aortic Endothelial Cells (HAOEC) 40-85%  80-95%
  Human Coronary Artery Endothelial Cells (HCAEC)    
  Human Dermal Lymphatic Microvascular Endothelial Cells (HDLMVEC)    
  Human Dermal Microvascular Endothelial Cells (HDMVEC)    
  Human Lung Microvascular Endothelial Cells (HLMVEC)    
  Human Pulmonary Artery Endothelial Cells (HPAEC)    
  Human Umbilical Vein Endothelial Cells (HUVEC)    
  Bovine Aortic Endothelial Cells (BAOEC)    
  Bovine Coronary Artery Endothelial Cells (BCAEC)    
  Bovine Pulmonary Artery Endothelial Cells (BPAEC)    
  Porcine Pulmonary Artery Endothelial Cells (PPAEC)    
  Rat Aortic Endothelial Cells (RAOEC)    
  Rat Brain Microvascular Endothelial Cells (RBMVEC)    
       
Epithelial Human Bronchial Epithelial Cells (HBEpC) 40-70%  70-95%
  Human Epidermal Keratinocytes (HEK)    
  Human Mammary Epithelial Cells (HMEpC)    
  Human Prostate Epithelial Cells (HPrEpC)    
  Human Tracheal Epithelial Cells (HTEpC)    
  Human Umbilical Epithelial Cells    
       
Fibroblast Human Cardiac Fibroblasts (HCF) 30-70%  90-95%
  Human Dermal Fibroblasts (HDF)    
  Human Lung Fibroblasts (HLF)    
       
Hepatocyte Rat Hepatocytes (RH)  40-60%  70-95%
       
HUVEC Human Umbilical Vein Endothelial Cells (HUVEC)  40-85%  80-95%
       
Neuron Rat Cortical Neurons (RCoN)  10-20%   80-95%
       
Preadipocyte Human Preadipocytes (HPAd)  25%   80-95%
       
Skeletal Muscle Human Skeletal Muscle Cells (HSkMC)  15-30%   80-95%
       
Smooth Muscle Human Aortic Smooth Muscle Cells (HAOSMC)  35-65%  80-95%
  Human Brachiocephalic Artery Smooth Muscle Cells (HBcASMC)    
  Human Carotid Artery Smooth Muscle Cells (HCtASMC)    
  Human Coronary Artery Smooth Muscle Cells (HCASMC)    
  Human Internal Thoracic Artery Smooth Muscle Cells (HITASMC)    
  Human Pulmonary Artery Smooth Muscle Cells (HPASMC)    
  Human Subclavian Artery Smooth Muscle Cells (HScASMC)    
  Human Umbilical Vein Smooth Muscle Cells (HUVSMC)