Human Umbilical Vein Endothelial Cell (HUVEC) are (A), immunolabeled with VEGFR2 antibodies (green, B), stained with EC-specific DiI-Ac-LDL (red, C), and forming vessel-like structures (green) when cultured with HDF in the presence of VEGF. Nuclei are visualized with PI (red, B) or DAPI (blue, D).
Human Umbilical Vein Endothelial Cells: HUVEC
Description
Human Umbilical Vein Endothelial Cells (HUVEC)Â provide a classic model system to study many aspects of endothelial function and disease, such as normal, abnormal and tumor-associated angiogenesis, oxidative stress, hypoxia and inflammation related pathways in endothelia under normal and pathological conditions, cardiovascular-related complications associated with various diseases, mode of action and cardiovascular protection effects of various compounds, etc.
Select HUVEC lots have been tested to demonstrate stimulation-dependent angiogenesis and key endothelial cell signaling pathways (phosphorylation of VEGFR2). More information about pre-screened endothelial cells can be found on the Pre-Screened Endothelial Cell Product Page.
HUVEC from Cell Applications, Inc. have been utilized in numerous research publications, for example, to:
- Understand anti-inflammatory properties of HDL
- Develop scaffolds for tissue engineering
- Characterize endothelial cell response to stress and discover mechanisms that protect mitochondria against oxidative stress
- Demonstrate that prolonged hypoxia induces MMP-1 expression, EC migration and angiogenesis
- Show that Alzheimer’s β-amyloid peptide exhibits anti-angiogenic properties
- Demonstrate for the first time that mature miRNA can control gene expression in a cell where it is neither transcribed nor processed
- Show that abnormal matrix composition characteristic for systemic sclerosis leads to impaired vascular function and angiogenesis
- Discover novel mechanisms of inhibiting tumor angiogenesis and metastasis, by limiting EC migration and proliferation, as well as the expression of growth factors and enzymes
- Develop a bubble lyposome based system that enables therapeutic miRNA delivery
- Demonstrate that commercially used flame retardants cause oxidative stress
- Show that hyperglycemia leads to endothelial dysfunction, partially due to depletion of antioxidants, and that regaining glycemic control normalizes growth factor levels in ischemia and improves perfusion recovery
- Show that inhibition of aldose reductase inhibits migration and formation of capillary-like structures by endothelial cells
- Investigate effects of molecular mobility of the outmost material surfaces on cellular adhesion and organization
- Identify signaling differences in cells cultured on different ECM components.
- Investigate mechanisms of cardiovascular protection exerted by bioactive plant and fungal components
- Show that in low doses coumaric acid and resveratrol provide beneficial effects and protect endothelial cells from reactive oxygen species
- Show that combined treatment with melatonin and atorvastatin reversed damage to EC, partly by reducing free radical generation and lipid peroxidation
- Discover that deferoxamine (DFO), an iron-chelating agent, mitigates deleterious effects of radiation on angiogenesis
- Study the differential effects of wood smoke and diesel exhaust particles, as well as carbon nanotubes on inducing oxidative stress and production of cytokines and adhesion molecules in endothelia
Details
| Tissue | Healthy human umbilical vein | |
|---|---|---|
| QC | No bacteria, yeast, fungi, mycoplasma, virus | |
| Character | Factor VIII-related Ag, DiI-Ac-LDL uptake. S-HUVEC are select HUVEC lots that have been tested positive for VEGFR2 pathway activation following stimulation by VEGF. | |
| Bioassay | Attach, spread, proliferate in Growth Med | |
| Cryovial | 500,000 HUVEC (primary culture) in Bas Med w/ 10% FBS, 10% DMSO | |
| Kit | Cryovial frozen HUVEC (200-05n), Growth Medium (211-500), Subculture Rgnt Kit (090K) | |
| Proliferating | Shipped in Tsfr Med, 1st psg (flasks or plates) | |
| Doublings | At least 16 | |
| Applications | Laboratory research use only (RUO). Not for human, clinical, diagnostic or veterinary use. |
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