MSDS Cryopreserved Cells
Instructions HBEpC Normal
Cell Apps Flyer Epithelial Cells
5 Important Cell Culture Rules
Cell Apps Poster Primary Cells
Cell Applications Inc Brochure

Description

Human Bronchial Epithelial Cells (HBEpC) provide an excellent model system to study all aspects of epithelial function and disease, particularly those related to airway viral infections, as well as tissue repair mechanisms, signaling changes and potential treatments relevant to lung injuries, mechanical and oxidative stress, inflammation, pulmonary diseases and smoking. When grown on inserts and provided with the liquid/air interface, HBEpC can differentiate into a pseudostriated epithelium and serve as a more physiological 3D tissue model for in vitro studies. The HBEpC shown here were cultured (L) and immunolabeled for cytokeratin 18 (R). 
HBEpC from Cell Applications, Inc. have been used to examine:
  • Activation, expression and production of genes, kinases and signaling pathways by cytokines, growth factors, interleukins, binding proteins and pro-inflammatory molecules. 
  • Stimulation-dependent, observable changes in proliferation, bronchial epithelial permeability, crosslinking of membrane glycoproteins and cell surface adhesion molecules.  Drug discovery cell screening for in vitro assay of compounds, or to extend and confirm high-throughput work done in cell lines.
  • Clinical focused discoveries leveraging HBEpC include therapeutics to suppress tumor gene transcription, apoptosis, inflammation, auto-immune disease and viral infection, while enhancing cell protection, repair and lifespan.
Characterization: Morphology consistent with epithelial origin, and positive for epithelial cell marker cytokeratin 18

(Click to Enlarge) Human Bronchial Epithelial Cell (HBEpC)-based 3D airway tissue model, showing Millipore Insert, day 14 (A).  High resolution confocal imaging (B–G) of the top cell layer (B,D,F) or a cross section (C,E,G).  DAPI-labeled Nuclei (B&C), rhod-phall-labeled Actin (D&E) and merged images (F&G).

Details

TissueNormal healthy human bronchial epithelium
QCNo bacteria, yeast, fungi, mycoplasma, virus
BioassayAttach, spread, proliferate in Growth Med
Cryovial500,000 HBEpC (1st passage) frozen in Basal Med w/ 10% FBS, 10% DMSO
KitCryovial frozen HBEpC (502-05a), Gr Med (512-500), Subculture Rgnt Kit (090K)
ProliferatingShipped in Gr Med, 3rd psg (flasks or plates)
DoublingsAt least 16
ApplicationsLaboratory research use only (RUO). Not for human, clinical, diagnostic or veterinary use.
Instructions HBEpC Normal

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MSDS Cryopreserved Cells

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Products

Product Size CAT.# Price Quantity
Cryopreserved HBEpC, Adult: 5x10^5 Cells (Adult), Medium & Subculture Reagents (See Details tab for specifics) Size: 1 Kit CAT.#: 502K-05a Price: $868.00
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Cryopreserved HBEpC, Adult: Frozen HBEpC (5x10^5) Size: 1 Cryovial CAT.#: 502-05a Price: $695.00
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Cryopreserved HBEpC-AS, Adult: Frozen HBEpC-AS from donor with Asthma (5x10^5) Size: 1 Cryovial CAT.#: 502AS-05a Price: $845.00
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Cryopreserved HBEpC-AS, Adult: 5x10^5 Cells (from donor with Asthma, Adult), Medium & Subculture Reagents (See Details tab for specifics) Size: 1 Kit CAT.#: 502ASK-05a Price: $941.00
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Cryopreserved HBEpC-COPD, Adult: Frozen HBEpC-COPD from donor with Chronic Obstructive Pulmonary Disease (5x10^5) Size: 1 Cryovial CAT.#: 502COPD-05a Price: $845.00
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Cryopreserved HBEpC-COPD, Adult: 5x10^5 Cells (from donor with COPD, Adult), Medium & Subculture Reagents (See Details tab for specifics) Size: 1 Kit CAT.#: 502COPDK-05a Price: $1,011.00
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Proliferating HBEpC, Adult: Actively growing, dividing cells in medium Size: T-25 Flask CAT.#: 503-25a Price: $695.00
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Proliferating HBEpC, Adult: Actively growing, dividing cells in medium Size: T-75 Flask CAT.#: 503-75a Price: $900.00
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Proliferating HBEpC, Adult: Actively growing, dividing cells in medium Size: 6 Well CAT.#: 503-6Wa Price: $900.00
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Proliferating HBEpC, Adult: Actively growing, dividing cells in medium Size: 96 Well CAT.#: 503-96Wa Price: $1,029.00
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Related Products

Product Size CAT.# Price Quantity
HBrEpC/HTrEpC Basal Medium: Without growth supplement (GS).  Add GS before use. Size: 500 ml CAT.#: 510-500 Price: $62.00
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HBrEpC/HTrEpC Differentiation Medium: Promotes cells to change from one type to another, more specialized Size: 250 ml CAT.#: 511D-250 Price: $129.00
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HBrEpC/HTrEpC Growth Medium: All-in-one ready-to-use Size: 500 ml CAT.#: 511-500 Price: $126.00
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HBrEpC/HTrEpC Growth Medium Kit: Basal medium & growth supplement sold together packaged separately Size: Kit CAT.#: 511K-500 Price: $125.00
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HBrEpC/HTrEpC Growth Medium wo Antibiotics: Growth medium without antibiotics Size: 500 ml CAT.#: 511A-500 Price: $125.00
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HBrEpC/HTrEpC Growth Supplement: Added to Basal Medium to create Growth Medium Size: 5 ml CAT.#: 511-GS Price: $62.00
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Human EpiVita Basal Medium: Basal medium (contains no growth supplement).  Add GS before use. Size: 500 ml CAT.#: 140-500 Price: $74.00
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Extended Family Products

Product Size CAT.# Price Quantity
3-D Airway Model: HBEpC differentiated into pseudostriated epithelium on PCF inserts Size: 12 Inserts CAT.#: 502-3D-12 Price: $769.00
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3-D Airway Model: HBEpC differentiated into pseudostriated epithelium on PCF inserts Size: 24 Inserts CAT.#: 502-3D-24 Price: $1,355.00
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3-D Airway Model : Cells, Media, Reagents & Inserts (See Details tab for specifics) Size: 1 Kit CAT.#: 502K-3D Price: $769.00
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Cyto-X Cell Counting Reagent: 500 tests Size: 1 Bottle CAT.#: 028-01 Price: $139.00
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Cyto-X Cell Counting Reagent Sample: 100 tests Size: Sample CAT.#: 028-S Price: $36.00
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Cytofect™ Epithelial Cell Transfection Kit: 124 x 24-Well Rxns Size: 1 Kit CAT.#: TF102K Price: $404.00
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Cytofect™ Epithelial Cell Transfection Kit: 25 x 24-Well Rxns Size: 1 Sample Kit CAT.#: TF102KS Price: $54.00
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Freezing Medium: For general cryopreservation of most primary cells. Contains FBS & DMSO. Size: 50 ml CAT.#: 040-50 Price: $54.00
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HBEpC RNA, Adult: Total RNA prepared from Human Bronchial Epithelial Cells, adult Size: 10 ug CAT.#: 502-R10a Price: $350.00
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HBEpC RNA, Adult: Total RNA prepared from Human Bronchial Epithelial Cells, adult Size: 25 ug CAT.#: 502-R25a Price: $700.00
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Human EGF, Animal-Free: Human Epidermal Growth Factor, Animal-Free Size: 500 ug CAT.#: RP1026AF-500 Price: $213.00
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Human EGF, Animal-Free: Human Epidermal Growth Factor, Animal-Free Size: 1000 ug CAT.#: RP1026AF-1000 Price: $290.00
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Human EGF, Animal-Free: Human Epidermal Growth Factor, Animal-Free Size: 100 ug CAT.#: RP1026AF-100 Price: $95.00
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Human Epidermal Growth Factor (EGF): Human Epidermal Growth Factor Size: 100 ug CAT.#: RP1026-100 Price: $86.00
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Human Epidermal Growth Factor (EGF): Human Epidermal Growth Factor Size: 500 ug CAT.#: RP1026-500 Price: $194.00
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Human Epidermal Growth Factor (EGF): Human Epidermal Growth Factor Size: 1000 ug CAT.#: RP1026-1000 Price: $264.00
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Human IL-1 alpha ELISA Kit: Human Interleukin-1 alpha ELISA Kit Size: 96 wells CAT.#: CL0389 Price: $484.00
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Human IL-1 beta ELISA Kit: Human Interleukin-1 beta ELISA Kit Size: 96 wells CAT.#: CL0392 Price: $587.00
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Human Interleukin-1-Alpha (IL-1 alpha): Human Interleukin-1-alpha Size: 10 ug CAT.#: RP1173-10 Price: $194.00
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Human Interleukin-1-Alpha (IL-1 alpha): Human Interleukin-1-alpha Size: 100 ug CAT.#: RP1173-100 Price: $624.00
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Human Interleukin-1-Alpha (IL-1 alpha): Human Interleukin-1-alpha Size: 1000 ug CAT.#: RP1173-1000 Price: $5,327.00
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Human Lung RNA: Total RNA prepared from human lung tissue Size: 50 ug CAT.#: 1H40-50 Price: $139.00
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Human Lung RNA: Total RNA prepared from human lung tissue Size: 250 ug CAT.#: 1H40-250 Price: $522.00
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Monoclonal IL-1 Receptor Antagonist Antibody: Monoclonal IL-1 Receptor Antagonist Antibody Size: 100 ul CAT.#: CB19846 Price: $302.00
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Subculture Reagent Kit: 100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution Size: 1 Kit CAT.#: 090K Price: $55.00
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Resources/Documents

Cell Apps Flyer Epithelial Cells

Format: PDF

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5 Important Cell Culture Rules

Format: PDF

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Cell Apps Poster Primary Cells

Format: PDF

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Cell Applications Inc Brochure

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Publications

2017

Shah, F., Stepan, A.F., O'Mahony, A., Velichko, S., Folias, A.E., Houle, C., Shaffer, C.L., Marcek, J., Whritenour, J., Stanton, R. and Berg, E.L., 2017. Mechanisms of Skin Toxicity Associated with Metabotropic Glutamate Receptor 5 Negative Allosteric Modulators.Cell Chemical Biology, 858-869.e5.

Chakraborty, S., V. Castranova, M. Perez and G. Piedimonte. 2017. Nanoparticles-induced apoptosis of human airway epithelium is mediated by proNGF/p75NTR signaling. J Toxicol & Env Hlth, Pt A, 80:53-68.
2016
Hiraku, Y., F. Guo, N. Ma, T. Yamada, S. Wang, S. Kawanishi and M. Murata. 2016. Multi-walled carbon nanotube induces nitrative DNA damage in human lung epithelial cells via HMGB1-RAGE interaction and Toll-like receptor 9 activation. Particle & Fibre Toxicol, 13:16.
Mungunsukh, O., Y. Lee, D. Bottaro and R. Day. 2016. The Hepatocyte Growth Factor Isoform NK2 Activates Motogenesis and Survival but not Proliferation due to Lack of Akt Activation. Cellular Signaling, 28:1114-1123.
Othumpangat, S., J. Noti, C. McMillen and D. Beezhold. 2016. ICAM-1 regulates the survival of influenza virus in lung epithelial cells during the early stages of infection. Virology, 487:85-94.
Perez, D. and H. Chen. 2016. Methods and Compositions for in vivo Immune Stimulation and Antigen Production. Patent Application US 20160022807 A1.
2015
Burns, T., A. Ali, and D. Matesic. 2015. Comparative Effects of 4-Phenyl-3-Butenoic Acid and Vorinostat on Cell Growth and Signaling. Anticancer Research, 35: 775-784.
Li, J., J. Guan X. Long and X. Xiang. 2015. Endothelin-1 Upregulates the Expression of High Mobility Group Box 1 in Human Bronchial Epithelial Cells. Pharmacology, 96:144-150.
Othumpangat, S., J. Noti, C. McMillen, and D. Beezhold. 2015. ICAM-1 regulates the survival of influenza virus in lung epithelial cells during the early stages of infection. Virology, 487:85-94.
2014
Chen, H., M. Angel, W. Li, C. Finch, A. Gonzalez, T. Sutton, J. Santos, and D. Perez. 2014. All-in-One Bacmids: an Efficient Reverse. J Virol, 88:10013.
Haselmayer, P., M. Camps, M. Muzerelle, S. El_bawab, C. Waltzinger, L. Bruns, N. Abla, M. Polokoff, C. Jond-necand, and M. Gaudet. 2014. Characterization of novel PI3Kδ inhibitors as potential therapeutics for SLE and lupus nephritis in pre-clinical studies. Name: Frontiers in Immunology. 5:233.
Hattori, S., K. Kojima, K. Minoshima, M. Yamaha, M. Horie, T. Sawamura, A. Kikuchi, and T. Deguchi. 2014. Detection of bladder cancer by measuring CD44v6 expression in urine with real-time quantitative reverse transcription polymerase chain reaction. Urology, 83:1443.e9-1443.e15.
Nasreen, N., L. Gonzalves, S. Peruvemba and K. Mohammed. 2014. Fluticasone furoate is more effective than mometasone furoate in restoring tobacco smoke inhibited SOCS-3 expression in airway epithelial cells. Intl Immunopharmacol, 19:153-160.
Prakash, S., S. Agrawal, H. Vahed, M. Ngyuen, L. BenMohamad, S. Gupta and A. Agrawal. 2014. Dendritic cells from aged subjects contribute to chronic airway inflammation by activating bronchial epithelial cells under steady state. Mucosal Immunol, 7:1386-1394.
Tripathi, B., X. Qian, P. Mertins, D. Wang, A. Papageorge, S. Carr, and D. Lowy. 2014. CDK5 is a major regulator of the tumor suppressor DLC1. JCB, 207:627-642.
Voiles, L., D. Lewis, L. Han, I. Lupov, T. Lin, M. Robertson, I. Petrache and H. Chang. 2014. Overexpression of type VI collagen in neoplastic lung tissues. Oncology Reports, 32:1897-1904.
2012
Abdullah, L., C. Wolber, M. Kesimer, J. Sheehan, and C.W. Davis. 2012. Studying Mucin Secretion from Human Bronchial Epithelial Cell Primary Cultures. In Mucins. Vol. 842. M.A. McGuckin and D.J. Thornton, editors. Humana Press. 259-277.
Narisawa-Saito, M., Y. Inagawa, Y. Yoshimatsu, K. Haga, K. Tanaka, N. Egawa, S. Ohno, H. Ichikawa, T. Yugawa, M. Fujita and T. Kiyono. 2012. A critical role of MYC for transformation of human cells by HPV16 E6E7 and oncogenic HRAS. Carcinogenesis, 33:910-917.
Nasreen, N., N. Khodayari, B. Sukka-Ganesh, S. Peruvemba, and K.A. Mohammed. 2012. Fluticasone propionate and Salmeterol combination induces SOCS-3 expression in airway epithelial cells. International Immunopharmacology. 12:217-225.
Othumpangat, S., M. Regier and G. Piedimonte. 2012. Nerve growth factor modulates human rhinovirus infection in airway epithelial cells by controlling ICAM-1 expression. Am J. Physiol – Lung Cellular & Molec Physiol, 302:L1057-L1066.
Othumpangat, S., C. Walton, and G. Piedimonte. 2012b. MicroRNA-221 Modulates RSV Replication in Human Bronchial Epithelium by Targeting NGF Expression. PloS one. 7:e30030.
2010
Berg, E.L., J. Yang, J. Melrose, D. Nguyen, S. Privat, E. Rosler, E.J. Kunkel, and S. Ekins. 2010. Chemical target and pathway toxicity mechanisms defined in primary human cell systems. Journal of Pharmacological and Toxicological Methods. 61:3-15.
Lung, J., K.-J. Liu, J.-Y. Chang, S.-J. Leu, and N.-Y. Shih. 2010. MBP-1 is efficiently encoded by an alternative transcript of the ENO1 gene but post-translationally regulated by proteasome-dependent protein turnover. FEBS Journal. 277:4308-4321.
Maier, K.G., X. Han, B. Sadowitz, K.L. Gentile, F.A. Middleton, and V. Gahtan. 2010. Thrombospondin-1: a proatherosclerotic protein augmented by hyperglycemia. Journal of Vascular Surgery. 51:1238-1247.
Mungunsukh, O., A.J. Griffin, Y.H. Lee, and R.M. Day. 2010. Bleomycin induces the extrinsic apoptotic pathway in pulmonary endothelial cells. American Journal of Physiology - Lung Cellular and Molecular Physiology. 298:L696-L703.
Tai, H.Y., M.F. Tam, H. Chou, D.W. Perng, and H.D. Shen. 2010. Pen ch 13 Major Fungal Allergen Decreases CD44 Expression in Human Bronchial Epithelial Cells. International Archives of Allergy and Immunology. 153:367-371.
2009
Houck, K.A., D.J. Dix, R.S. Judson, R.J. Kavlock, J. Yang, and E.L. Berg. 2009. Profiling Bioactivity of the ToxCast Chemical Library Using BioMAP Primary Human Cell Systems. Journal of biomolecular screening. 14:1054-1066.
Schembri, F., S. Sridhar, C. Perdomo, A.M. Gustafson, X. Zhang, A. Ergun, J. Lu, G. Liu, X. Zhang, J. Bowers, C. Vaziri, K. Ott, K. Sensinger, J.J. Collins, J.S. Brody, R. Getts, M.E. Lenburg, and A. Spira. 2009. MicroRNAs as modulators of smoking-induced gene expression changes in human airway epithelium. PNAS. doi: 10.1073/pnas.0806383106.
Song, H., H. Wan, Y. Araya, and D. Perez. 2009. Partial direct contact transmission in ferrets of a mallard H7N3 influenza virus with typical avian-like receptor specificity. Virology Journal. 6:126.
Tanaka, H., T. Fukushima, K. Yorita, M. Kawaguchi, and H. Kataoka. 2009. Tissue injury alters the site of expression of hepatocyte growth factor activator inhibitor type 1 in bronchial epithelial cells. Human Cell. 22:11-17.
Tumpey, and F. Fang. 2009. Novel Pandemic Influenza A(H1N1) Viruses Are Potently Inhibited by DAS181, a Sialidase Fusion Protein. PloS one. 4:e7788.
2010
Berg, E.L., J. Yang, J. Melrose, D. Nguyen, S. Privat, E. Rosler, E.J. Kunkel, and S. Ekins. 2010. Chemical target and pathway toxicity mechanisms defined in primary human cell systems. Journal of Pharmacological and Toxicological Methods. 61:3-15.
Lee, Y.H., O. Mungunsukh, R.L. Tutino, A.P. Marquez, and R.M. Day. 2010. Angiotensin-II-induced apoptosis requires regulation of nucleolin and Bcl-xL by SHP-2 in primary lung endothelial cells. Journal of cell science. 123:1634-1643.
Lung, J., K.-J. Liu, J.-Y. Chang, S.-J. Leu, and N.-Y. Shih. 2010. MBP-1 is efficiently encoded by an alternative transcript of the ENO1 gene but post-translationally regulated by proteasome-dependent protein turnover. FEBS Journal. 277:4308-4321.
Mungunsukh, O., A.J. Griffin, Y.H. Lee, and R.M. Day. 2010. Bleomycin induces the extrinsic apoptotic pathway in pulmonary endothelial cells. American Journal of Physiology - Lung Cellular and Molecular Physiology. 298:L696-L703.
Tai, H.Y., M.F. Tam, H. Chou, D.W. Perng, and H.D. Shen. 2010. Pen ch 13 Major Fungal Allergen Decreases CD44 Expression in Human Bronchial Epithelial Cells. International Archives of Allergy and Immunology. 153:367-371.
2008
Lee, Y.H., Y.J. Suzuki, A.J. Griffin, and R.M. Day. 2008. Hepatocyte growth factor regulates cyclooxygenase-2 expression via β-catenin, Akt, and p42/p44 MAPK in human bronchial epithelial cells. American Journal of Physiology. 294:L778-L786.
2007
Haga, K., S.-i. Ohno, T. Yugawa, M. Narisawa-Saito, M. Fujita, M. Sakamoto, D.A. Galloway, and T. Kiyono. 2007. Efficient immortalization of primary human cells by p16INK4a-specific short hairpin RNA or Bmi-1, combined with introduction of hTERT. Cancer science. 98:147-154.
Handa, K., T. Yugawa, M. Narisawa-Saito, S.-i. Ohno, M. Fujita, and T. Kiyono. 2007. E6AP-Dependent Degradation of DLG4/PSD95 by High-Risk Human Papillomavirus Type 18 E6 Protein. Journal of Virology. 81:1379-1389.
Horvath, G., E.S. Mendes, N. Schmid, A. Schmid, G.E. Conner, M. Salathe, and A. Wanner. 2007. The effect of corticosteroids on the disposal of long-acting β2-agonists by airway smooth muscle cells. Journal of Allergy and Clinical Immunology. 120:1103-1109.
2006
Tai, H., M. Tam, H. Chou, H. Peng, S. Su, D. Perng, and H. Shen. 2006. Pen ch 13 allergen induces secretion of mediators and degradation of occludin protein of human lung epithelial cells. 61:382-388.
2005
Lee, S.C., J.Y. Hsu, L.S. Fu, J.J. Chu, S.J. Fan, and C.S. Chi. 2005. Comparison of the activities of granulocyte-macrophage colony-stimulating factor and interleukin-8 secretion between two lung epithelial cell lines. J. microbial., immunol.& infection. 38:327-331.
Look, D.C., L.L. Stoll, S.A. Romig, A. Humlicek, B.E. Britigan, and G.M. Denning. 2005. Pyocyanin and Its Precursor Phenazine-1-Carboxylic Acid Increase IL-8 and Intercellular Adhesion Molecule-1 Expression in Human Airway Epithelial Cells by Oxidant-Dependent Mechanisms. The Journal of Immunology. 175:4017-4023.
2004
Kilani, M., K. Mohammed, N. Nasreen, R. Tepper, and V. Antony. 2004a. RSV Causes HIF-1α Stabilization via NO Release in Primary Bronchial Epithelial Cells. Inflammation. 28:245-251.
Kilani, M.M., K.A. Mohammed, N. Nasreen, J.A. Hardwick, M.H. Kaplan, R.S. Tepper, and V.B. Antony. 2004b. Respiratory syncytial virus causes increased bronchial epithelial permeability*. CHEST Journal. 126:186-191.
2003
O'Malley, Y.Q., K.J. Reszka, G.T. Rasmussen, M.Y. Abdalla, G.M. Denning, and B.E. Britigan. 2003. The Pseudomonas secretory product pyocyanin inhibits catalase activity in human lung epithelial cells. American Journal of Physiology. 285:L1077-L1086.