Instructions HPAd Normal
5 Important Cell Culture Rules
MSDS Cryopreserved Cells
Cell Apps Flyer Adipocytes
Cell Apps Poster Primary Cells
Cell Applications Inc Brochure
Human Preadipocytes (HPAd) provide ideal cell models and facilitate life science research and drug discovery for obesity, diabetes and cardiovascular diseases. We derive HPAd from human subcutaneous and heart adipose tissue and cryopreserve these fibroblast-like precursor cells at the end of primary culture. They can propagate two passages prior to differentiating into Human Adipocytes (HAd). Complete differentiation can be easily achieved through the use of Preadipocyte Differentiation Medium. Mature HAd are expected 10 days after induction of differentiation and should remain healthy and responsive for at least 2 weeks after complete differentiation. Adipose mass can be controlled by inhibition of HPAd differentiation and increase of lypolysis. HPAd are also available prescreened for expression of adipocyte markers.
HPAd from Cell Applications, Inc. have been used in multiple studies investigating the cellular basis of diabetes and obesity, as well as mechanisms of action of various drug candidates. For example, it was shown that:
- Interaction between infiltrating monocytes and adipocytes affects production of metalloproteinases and osteopontin, a proinflammatory cytokine, which ultimately leads to development of more adipose tissue and insulin resistance
- Adipocyte differentiation requires activation of Akt1 through the mTORC2-BSTA mechanism, leading to downstream suppression of FoxC2
- Instead of protecting from hyperglycemia-induced ER stress, like it does in other cells, in adipocytes a bioactive, endogenously produced compound taurine was shown to modulate the expression of adipokines under inflammatory conditions by inhibiting the STAT-3 signaling pathway (Kim, 2013a,c), and to inhibit differentiation of preadipocytes into adipocytes
- FGF21, which leads to reduction of body weight in animal models of obesity, was shown to act by modulating gene expression, phosphorylating Frs2a, Erk1/2, and Mypt1 and by increasing oxidative capacity of adipocytes via AMPK–SIRT1–PGC1a cascade
- Atrial natriuretic peptide (ANP) regulates lipid catabolism and reduce insulin resistance in HPAd by activating AMPK
- Adipocytokines are involved in normal pregnancy and pregnancy-induced hypertension
- Lotus seed extract exhibits anti-obesity and hypolipidaemic properties
- HPAd (along with our Human Dermal Fibroblasts) demonstrate the role of epigenetic modifications in increasing efficiency of iPS reprogramming
Normal healthy human adipose tissue
|No bacteria, yeast, fungi, mycoplasma, virus|
|Positive for lipid drops in cytoplasm & Oil Red O Staining, in Differentiation Med|
|Attach, spread, proliferate in Growth Med|
500,000 HPAd (2nd passage) frozen in Basal Medium w/ 20% FBS, 5% DMSO
Cryovial frozen HPAd (802h/s-05a), Growth Medium (811-500), Subcultr Rgnt Kit (090K)
Shipped in Gr Med, 3rd psg (flasks or plates)
Can be cultured 2 passages before differentiation into HAd
Laboratory research use only (RUO). Not for human, clinical, diagnostic or veterinary use.
|Cryopreserved HPAd, Heart, Adult: 5x10^5 Cells (from Heart Tissue, Adult), Medium & Subculture Reagents (See Details tab for specifics)||Size: 1 Kit||CAT.#: 802hK-05a||Price: $652.00|
|Cryopreserved HPAd, Heart, Adult: Frozen HPAd from Heart Tissue (5x10^5)||Size: 1 Cryovial||CAT.#: 802h-05a||Price: $472.00|
|Cryopreserved HPAd, Subcutaneous, Adult: 5x10^5 Cells (from Subcutaneous Tissue, Adult), Medium & Subculture Reagents (See Details tab for specifics)||Size: 1 Kit||CAT.#: 802sK-05a||Price: $575.00|
|Cryopreserved HPAd, Subcutaneous, Adult: Frozen HPAd from Subcutaneous Tissue (5x10^5)||Size: 1 Cryovial||CAT.#: 802s-05a||Price: $395.00|
|Cryopreserved HPAd-h-AS, Adult, heart: Frozen HPAd-h-AS from Heart Tissue of donor with Asthma, Adult (5x10^5)||Size: 1 Cryovial||CAT.#: 802hAS-05a||Price: $522.00|
|Cryopreserved HPAd-h-AS, Adult, heart: 5x10^5 Cells (from Heart Tissue of donor with Asthma, Adult), Medium & Subculture Reagents (See Details tab for specifics)||Size: 1 Kit||CAT.#: 802hASK-05a||Price: $702.00|
|Cryopreserved HPAd-h-T2D, Heart, Adult: Frozen HPAd from Heart Tissue of donor with Type 2 Diabetes (5x10^5)||Size: 1 Cryovial||CAT.#: 802hT2D-05a||Price: $522.00|
|Cryopreserved HPAd-h-T2D, Heart, Adult: 5x10^5 Cells (from Heart Tissue of donor with Type 2 Diabetes, Adult), Medium & Subculture Reagents (See Details tab for specifics)||Size: 1 Kit||CAT.#: 802hT2DK-05a||Price: $702.00|
|Cryopreserved HPAd-s-T2D, Subcutaneous, Adult: Frozen HPAd from Subcutaneous Tissue of donor with Type 2 Diabetes (5x10^5)||Size: 1 Cryovial||CAT.#: 802sT2D-05a||Price: $334.00|
|Cryopreserved HPAd-s-T2D, Subcutaneous, Adult: 5x10^5 Cells (from Subcutaneous Tissue of donor with Type 2 Diabetes, Adult), Medium & Subculture Reagents (See Details tab for specifics)||Size: 1 Kit||CAT.#: 802sT2DK-05a||Price: $514.00|
|Proliferating HPAd, Adult, heart: Actively growing, dividing cells, from heart tissue, in medium||Size: T-25 Flask||CAT.#: 803h-25a||Price: $472.00|
|Proliferating HPAd, Adult, heart: Actively growing, dividing cells, from heart tissue, in medium||Size: T-75 Flask||CAT.#: 803h-75a||Price: $677.00|
|Proliferating HPAd, Adult, heart: Proliferating HPAd from heart tissue||Size: 24 Well||CAT.#: 803h-24Wa||Price: $677.00|
|Proliferating HPAd, Adult, heart: Actively growing, dividing cells, from heart tissue, in medium||Size: 96 Well||CAT.#: 803h-96Wa||Price: $806.00|
|Proliferating HPAd, Subcutaneous, Adult: Actively growing, dividing cells, from subcutaneous tissue, in medium||Size: T-25 Flask||CAT.#: 803s-25a||Price: $395.00|
|Proliferating HPAd, Subcutaneous, Adult: Actively growing, dividing cells, from subcutaneous tissue, in medium||Size: T-75 Flask||CAT.#: 803s-75a||Price: $585.00|
|Proliferating HPAd, Subcutaneous, Adult: Actively growing, dividing cells, from subcutaneous tissue, in medium||Size: 24 Well||CAT.#: 803s-24Wa||Price: $585.00|
|Size: 96 Well||CAT.#: 803s-96Wa||Price: $705.00|
|Proliferating HPAd, Subcutaneous, Pre-Screened, Adult: Actively growing, dividing cells from subcutaneous tissue, in medium, adult||Size: 96 Well||CAT.#: S803s-96Wa||Price: $852.00|
|Proliferating HPAd, Subcutaneous, Pre-Screened, Adult: Actively growing, dividing cells from subcutaneous tissue, in medium, adult||Size: 24 Well||CAT.#: S803s-24Wa||Price: $732.00|
|HPAd Growth Medium: All-in-one ready-to-use||Size: 500 ml||CAT.#: 811-500||Price: $135.00|
|HPAd Growth Medium Kit: Basal medium & growth supplement sold together packaged separately||Size: Yields 500 ml||CAT.#: 811K-500||Price: $148.00|
|HPAd Basal Medium: Basal medium (contains no growth supplement). Add GS before use.||Size: 500 ml||CAT.#: 810-500||Price: $77.00|
|HPAd Growth Supplement: Added to Basal Medium to create Growth Medium||Size: 50 ml||CAT.#: 811-GS||Price: $59.00|
Extended Family Products
|Freezing Medium: For general cryopreservation of most primary cells. Contains FBS & DMSO.||Size: 50 ml||CAT.#: 040-50||Price: $54.00|
|Cytofect™ Preadipocyte Transfection Kit: 175 x 24-Well Rxns||Size: 1 Kit||CAT.#: TF802K||Price: $350.00|
|Cytofect™ Preadipocyte Transfection Kit: 25 x 24-Well Rxns||Size: 1 Sample Kit||CAT.#: TF802KS||Price: $54.00|
|HPAd-S RNA, Adult: Total RNA prepared from Human Preadipocytes, Subcutaneous, adult||Size: 10 ug||CAT.#: 802s-R10a||Price: $350.00|
|HPAd-S RNA, Adult: Total RNA prepared from Human Preadipocytes, Subcutaneous, adult||Size: 25 ug||CAT.#: 802s-R25a||Price: $700.00|
|Human Leptin: Human Leptin||Size: 200 ug||CAT.#: RP1037-200||Price: $86.00|
|Human Leptin: Human Leptin||Size: 1000 ug||CAT.#: RP1037-1000||Price: $194.00|
|Human Leptin: Human Leptin||Size: 5000 ug||CAT.#: RP1037-5000||Price: $538.00|
|Human Leptin ELISA Kit: Human Leptin ELISA Kit||Size: 96 wells||CAT.#: CL0437||Price: $587.00|
|Subculture Reagent Kit: 100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution||Size: 1 Kit||CAT.#: 090K||Price: $63.00|
|HPAd Differentiation Medium: Add to preadipocytes and mesenchymal stem cells to differentiate into adipocytes||Size: 250 ml||CAT.#: 811D-250||Price: $95.00|
|HPAd Differentiation Medium, Component Kit: Differentiation medium components sold together packaged separately||Size: Yields 250 ml||CAT.#: 811DS-250||Price: $132.00|
|HAd Maintenance Medium: Maintenance medium for adipocytes||Size: 250 ml||CAT.#: 811M-250||Price: $68.00|
|HAd Maintenance Medium wo Insulin: Maintenance Medium for adipocytes without insulin||Size: 250 ml||CAT.#: 811Mi-250||Price: $78.00|
|Adipoccyte Starvation Medium: Use when cells need to be starved overnight to 24 hrs before experiment||Size: 250 ml||CAT.#: 811S-250||Price: $62.00|
Ignacio, R., C. Gibbs, E. Lee and D. Son. 2016. Differential Chemokine Signature between Human Preadipocytes and Adipocytes. Immune Network, 16:189-194.
Ono-Uruga, Y., K. Tozawa, T. Horiuchi, M. Murata, S. Okamoto, Y. Ikeda, T. Suda and Y. Matsubara. 2016. Human adipose tissue-derived stromal cells can differentiate into megakaryocytes and platelets by secreting endogenous thrombopoietin. J Thrombosis & Haemostasis, 6:1285-1297.
Subbaramaiah, K., K. Brown, H. Zahid, K. Balmus, R. Weiss, B. Herbert and A. Dannenberg. 2016. Hsp90 and PKM2 Drive the Expression of Aromatase in Li-Fraumeni Syndrome Breast Adipose Stromal Cells. J Biol Chem, 291:16011-16023.
Fuji, S., K. Fujimoto, N. Goto, M. Kanawa, T. Kawamoto, H. Pan, P. Srivatanakul, W. Rakdang, J. Pornprsitwech, T. Saskianti, K. Suardita, F. Nishimura, and Y. Kato. 2015. Characteristic expression of MSX1, MSX2, TBX2 and ENTPD1 in dental pulp cells. Biomedical Reports, 29 April.
Floyd, E., J. Staszkiewicz, R. Power, G. Kilroy, H. Kirk-Ballard, C. Barnes, K. Strickler, F. Rim, L. Harkins, R. Gao, J. Kim and K. Eilersten. 2015. Prolonged Proteasome Inhibition Cyclically Upregulates Oct3/4 and Nanog Gene Expression, but Reduces Induced Pluripotent Stem Cell Colony Formation. Cellular Reprogramming, 17:95-105.
Guo, F., H. He, Z. Fu, S. Huang, T. Chen, C. Papasian, L. Morse, Y. Xu, R. Battaglino, X. Yang, Z. Jiang, H. Xin, and M. Fu. 2015. Adipocyte-derived PAMM suppresses macrophage inflammation by inhibiting MAPK signaling. Biochem J, DOI: 10.1042/BJ20150019.
Ito, Y., H. Ishiguro, N. Kobayashi, H. Hasumi, M. Watanabe, M. Yao and H. Uemura. 2015. Adipocyte-derived monocyte chemotactic protein-1 (MCP-1) promotes prostate cancer progression through the induction of MMP-2 activity. The Prostate, 75:1009-1019.
Lynch, P. E. Thompson, K. McGinnis, Y. Rovira Gonzalez, J. Surdo, S. Bauer and D. Hursh. 2015. Chromatin Changes at the PPAR-γ2 Promoter During Bone Marrow-Derived Multipotent Stromal Cell Culture Correlate With Loss of Gene Activation Potential. Stem Cells, 33:2169-2181.
You, Y., S. Liu, L. Peng, M. Long, H. Deng and H. Zhao. 2015. Arg972 insulin receptor substrate‑1 enhances tumor necrosis factor‑α‑induced apoptosis in osteoblasts. Molec Med Repts, 12: ISSN:1791-3004.
Matsubara, Y., T. Zama, Y. Ikeda, Y. Uruga, T. Suda and S. Matsuoka. 2014. Method for producing megakaryocytes, platelets and/or thrombopoietin using mesenchymal cells. Patent Application US 20160177265 A1.
You, J., Y. Lee, K. Kim, S. Kim and K. Chang. 2014. Anti-obesity and hypolipidaemic effects of Nelumbo nucifera seed ethanol extract in human pre-adipocytes and rats fed a high-fat diet. J Science of Food & Ag, 94:568-575.
You, J., Y. Lee, K. Kim, S. Kim and K. Chang. 2014. Ethanol extract of lotus (Nelumbo nucifera) root exhibits an anti-adipogenic effect in human pre-adipocytes and anti-obesity and anti-oxidant effects in rats fed a high-fat diet. Nutrition Res, 34:258-267.
Kim, K., H.-I. Ji, and H.-I. Yang. 2013a. Taurine May Not Alleviate Hyperglycemia-Mediated Endoplasmic Reticulum Stress in Human Adipocytes. In Taurine 8. Vol. 775. A. El Idrissi and W.J. L'Amoreaux, editors. Springer New York. 395-403.
Kim, K.S., H.M. Choi, H.I. Ji, C. Kim, J.Y. Kim, R. Song, S.M. Kim, Y.A. Lee, S.H. Lee, H.I. Yang, M.C. Yoo, and S.J. Hong. 2013b. Effect of taurine chloramine on differentiation of human preadipocytes into adipocytes. In Advances in experimental medicine and biology. Vol. 775. 247-257.
Kim, K.S., H.I. Ji, H. Chung, C. Kim, S.H. Lee, Y.A. Lee, H.I. Yang, M.C. Yoo, and S.J. Hong. 2013c. Taurine chloramine modulates the expression of adipokines through inhibition of the STAT-3 signaling pathway in differentiated human adipocytes. Amino acids. 45:1415-1422.
Muise, E.S., S. Souza, A. Chi, Y. Tan, X. Zhao, F. Liu, Q. Dallas-yang, M. Wu, T. Sarr, L. Zhu, H. Guo, Z. Li, W. Li, W. Hu, G. Jiang, C.P. Paweletz, R.C. Hendrickson, J.R. Thompson, J. Mu, J.P. Berger, and H. Mehmet. 2013. Downstream Signaling Pathways in Mouse Adipose Tissues Following Acute In Vivo Administration of Fibroblast Growth Factor 21. PloS one. 8:e73011.
Yao, Y., M. Suraokar, B.G. Darnay, B.G. Hollier, T.E. Shaiken, T. Asano, C.-H. Chen, B.H.-J. Chang, Y. Lu, and G.B. Mills. 2013. BSTA Promotes mTORC2-Mediated Phosphorylation of Akt1 to Suppress Expression of FoxC2 and Stimulate Adipocyte Differentiation. Science signaling. 6:ra2.
You, J.S., Y.J. Lee, K.S. Kim, S.H. Kim, and K.J. Chang. 2013. Anti-obesity and hypolipidaemic effects of Nelumbo nucifera seed ethanol extract in human pre-adipocytes and rats fed a high-fat diet. J. Sci. Food & agriculture: doi: 10.1002/jsfa.6297.
Rim, J.S., K.L. Strickler, C.W. Barnes, L.L. Harkins, J. Staszkiewicz, J.M. Gimble, G.H. Leno, and K.J. Eilertsen. 2012. Temporal epigenetic modifications differentially regulate ES cell-like colony formation and maturation. Stem Cell Discovery. 2:45-57.
Naruse, K., Y. Yamasaki, T. Tsunemi, A. Onogi, T. Noguchi, T. Sado, H. Oi, and H. Kobayashi. 2011. Increase of high molecular weight adiponectin in hypertensive pregnancy was correlated with brain-type natriuretic peptide stimulation on adipocyte. Pregnancy Hypertension: An International Journal of Women's Cardiovascular Health. 1:200-205.
Samuvel, D.J., J. Jin, K.P. Sundararaj, Y. Li, X. Zhang, M.F. Lopes-Virella, and Y. Huang. 2011. TLR4 Activation and IL-6-Mediated Cross Talk between Adipocytes and Mononuclear Cells Synergistically Stimulate MMP-1 Expression. Endocrinology. 152:4662-4671.
Samuvel, D.J., K.P. Sundararaj, Y. Li, M.F. Lopes-Virella, and Y. Huang. 2010. Adipocyte-Mononuclear Cell Interaction, Toll-like Receptor 4 Activation, and High Glucose Synergistically Up-regulate Osteopontin Expression via an Interleukin 6-mediated Mechanism. Journal of Biological Chemistry. 285:3916-3927.
Asai-Sato, M., M. Okamoto, M. Endo, H. Yoshida, M. Murase, M. Ikeda, H. Sakakibara, T. Takahashi, and F. Hirahara. 2006. Hypoadiponectinemia in Lean Lactating Women: Prolactin Inhibits Adiponectin Secretion from Human Adipocytes. Endocrine J, 53:555-562.