Instructions RAOEC
5 Important Cell Culture Rules
MSDS Cryopreserved Cells
Cell Apps Fllyer Cardiovascular Cells
Cell Apps Flyer Endothelial Cells
Cell Apps Poster Primary Cells
Cell Applications Inc Brochure
Description
Rat Aortic Endothelial Cells (RAOEC) provide an excellent model system to study all aspects of cardiovascular function and disease, and they have been utilized in dozens of research publications. The cells test positive for DiI-Ac-LDL uptake.
(Click to Enlarge) Rat Aortic Endothelial Cells (RAOEC) monolayer (L) and labeled with DiI-Ac-LDL (red), the acetylated apoprotein specifically recognized by endothelial cell receptors (R).
Details
Tissue: | Normal healthy adult rat aorta |
| QC | No bacteria, yeast, fungi, mycoplasma |
| Character | Positive for DiI-Ac-LDL uptake |
| Bioassay | Attach, spread, proliferate in Growth Med |
Cryovial | 500,000 RAOEC (3rd passage) frozen in basal medium w/ 10% FBS, 10% DMSO |
Kit | Cryovial frozen RAOEC (R304-05a), Gr Med (R211-500), Attch Fct Sol (123-100), Subcltr Rgnt Kit (090K) |
Proliferating | Shipped in Gr Med, 4th psg (flasks or plates) |
Doublings | At least 6 |
Applications | Laboratory research use only (RUO). Not for human, clinical, diagnostic or veterinary use. |
Products
| Product | Size | CAT.# | Price | Quantity |
|---|---|---|---|---|
| Cryopreserved Rat Aortic Endothelial Cells Total Kit, adult: 5x10^5 Cells (Adult), Medium & Subculture Reagents (See Details tab for specifics) | Size: 1 Kit | CAT.#: R304K-05a | Price: $1,025.00 | |
| Cryopreserved Rat Aortic Endothelial Cells (RAOEC), adult: Frozen RAOEC (5x10^5) | Size: 1 Cryovial | CAT.#: R304-05a | Price: $823.00 | |
| Proliferating Rat Aortic Endothelial Cells (RAOEC), adult: Actively growing, dividing cells in medium | Size: T-25 Flask | CAT.#: R305-25a | Price: $823.00 | |
| Proliferating Rat Aortic Endothelial Cells (RAOEC), adult: Actively growing, dividing cells in medium | Size: T-75 Flask | CAT.#: R305-75a | Price: $1,013.00 | |
| Proliferating Rat Aortic Endothelial Cells (RAOEC), adult: Actively growing, dividing cells in medium | Size: 24 Well | CAT.#: R305-24Wa | Price: $1,013.00 | |
| Proliferating Rat Aortic Endothelial Cells (RAOEC), adult: Actively growing, dividing cells in medium | Size: 96 Well | CAT.#: R305-96Wa | Price: $1,133.00 |
Related Products
| Product | Size | CAT.# | Price | Quantity |
|---|---|---|---|---|
| Rat EC Basal Medium: Basal medium (contains no growth supplement). Add GS before use. | Size: 500 ml | CAT.#: R210-500 | Price: $77.00 | |
| Rat EC Growth Medium: All-in-one ready-to-use | Size: 500 ml | CAT.#: R211-500 | Price: $127.00 | |
| Rat EC Growth Medium Kit: Basal medium & growth supplement sold together packaged separately | Size: Yields 500 ml | CAT.#: R211K-500 | Price: $136.00 | |
| Rat EC Growth Medium Kit wo Phenol Red: Growth medium kit without phenol red | Size: Yields 500 ml | CAT.#: R211KPR-500 | Price: $156.00 | |
| Rat EC Growth Supplement: Added to Basal Medium to create Growth Medium | Size: 15 ml | CAT.#: R211-GS | Price: $77.00 |
Extended Family Products
| Product | Size | CAT.# | Price | Quantity |
|---|---|---|---|---|
| Attachment Factor Solution (AFS): AFS, contains Bovine Gelatin | Size: 100 ml | CAT.#: 123-100 | Price: $22.00 | |
| Freezing Medium: For general cryopreservation of most primary cells. Contains FBS & DMSO. | Size: 50 ml | CAT.#: 040-50 | Price: $54.00 | |
| Cytofect Endothelial Cell Transfection Kit (250 x 24-Wells): 250 x 24-Well Rxns | Size: 1 Kit | CAT.#: TF101K | Price: $496.00 | |
| Cytofect Endothelial Cell Transfection Sample Kit (25 x 24-Wells): 25 x 24-Well Rxns | Size: 1 Sample Kit | CAT.#: TF101KS | Price: $62.00 | |
| Cyto-X Cell Counting Reagent: 500 tests | Size: 1 Bottle | CAT.#: 028-01 | Price: $160.00 | |
| Cyto-X Cell Counting Reagent Sample: 100 tests | Size: Sample | CAT.#: 028-S | Price: $41.00 | |
| Rat Aortic Endothelial Cell RNA (RAOEC RNA), Adult : Total RNA prepared from Rat Aortic Endothelial Cells | Size: 10 ug | CAT.#: R304-R10a | Price: $520.00 | |
| Rat Aortic Endothelial Cell RNA (RAOEC RNA), Adult : Total RNA prepared from Rat Aortic Endothelial Cells | Size: 25 ug | CAT.#: R304-R25a | Price: $1,041.00 | |
| Subculture Reagent Kit: 100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution | Size: 1 Kit | CAT.#: 090K | Price: $63.00 | |
| Rat Vascular Endothelial Growth Factor-165 (VEGF-165): Rat Vascular Endothelial Growth Factor-165 | Size: 10 ug | CAT.#: RP3031-10 | Price: $195.00 | |
| Rat Vascular Endothelial Growth Factor-165 (VEGF-165): Rat Vascular Endothelial Growth Factor-165 | Size: 100 ug | CAT.#: RP3031-100 | Price: $484.00 | |
| Rat Vascular Endothelial Growth Factor-165 (VEGF-165): Rat Vascular Endothelial Growth Factor-165 | Size: 1000 ug | CAT.#: RP3031-1000 | Price: $4,090.00 |
Resources/Documents
Publications
2017
Iba, T., Sasaki, T., Ohshima, K., Sato, K., Nagaoka, I., Thachil, J., Bucur, S.Z., Levy, J.H., Despotis, G.J., Spiess, B.D. and Hillyer, C.D., 2017. The comparison of the protective effects of α-and β-antithrombin against vascular endothelial cell damage induced by histone in vitro. TH Open, 1(01), pp.e3-e10.
Bosc, L., J. Osmond, W. Giermakowska, C. Pace, J. Riggs, Ol Jackson-Weaver and N. Kanagy. 2017. NFAT Regulation of Cystathionine Gamma Lyase Expression in Endothelial Cells is Impaired in 2 Rats Exposed to Intermittent Hypoxia. Am J Physiol Heart Circ Physiol, doi:10.1152/ajpheart.00952.2015.
Masuda, Y., S. Yokose and H. Sakagami. 2017. Gene Expression Analysis of Cultured Rat-Endothelial Cells after Nd:YAG Laser Irradiation by Affymetrix GeneChip Array. In Vivo, 31:51-54.
Meyers, M., J. Rink, Q. Jiang, M. Kelly, J. Vercammen, C. Thaxton and M. Kibbe. 2017. Systemically administered collagen‐targeted gold nanoparticles bind to arterial injury following vascular interventions. Physiological Reports, 5:e13128.
2016
Bai, W., Z. Wu, S. Mitra and J. Brown. 2016. Effects of Multiwalled Carbon Nanotube Surface Modification and Purification on Bovine Serum Albumin Binding and Biological Responses. J Nanomater, ID 2159537.
Farwell, S., D. Kanyi, M. Hamel, J. Slee, E. Miller, M. Cipolle, and L. Lowe-Krentz. 2016. Heparin decreases in TNFα;-induced endothelial stress responses require transmembrane protein 184A and induction of dual specificity phosphatase-1. J Biol Chem, 291:5342-5354.
Iba, T., T. Hamakubo, I. Nagaoka, K. Sato and T. Jecko. 2016. Physiological levels of pentraxin 3 and albumin attenuate vascular endothelial cell damage induced by histone H3 in vitro. Microcirc, 23:240-247.
Kopaliani, I., M. Martin, B. Zatschler, B. Müller and A. Deussen. 2016. Whey peptide Isoleucine-Tryptophan inhibits expression and activity of matrix metalloproteinase-2 in rat aorta. Peptides, 82:52-59.
Kopaliani, I. M. Martin, B. Zatschler, B. Müller and A. Deussen. 2016. Data of the natural and pharmaceutical angiotensin-converting enzyme inhibitors as a potent blockers of matrix metalloproteinase-2 expression in rat aorta. Data in Brief, 8:958-962.
Otto, S., A. Deussen, B. Zatschler, B. Muller, A. Neisser, K. Barth, H. Morawietz and I. Kopaliani. 2016. A novel role of endothelium in activation of latent pro-membrane type 1 MMP and pro-MMP-2 in rat aorta. Cardiovascular Research, 109:409-418.
Shannahan, J., K. Fritz, A. Raghavendra, R. Podila and J. Brown. 2016. Disease-Induced Disparities in Formation of the Nanoparticle-Biocorona and the Toxicological Consequences. Toxicological Sciences, doi: 10.1093/toxsci/kfw097.
2015
Bahnson, E., N. Koo, N. Cantu-Medellin, A. Tsui, G. Havelka, J. Vercammen, Q. Jiang, E. Kelley and M. Kibbe. 2015. Nitric oxide inhibits neointimal hyperplasia following vascular injury via differential, cell-specific modulation of SOD-1 in the arterial wall. Nitric Oxide, 44:8-17.
Ding, Q., Y. Hussain, J. Chorazyczewski, R. Gros, and R. Feldman. 2015. GPER-independent effects of estrogen in rat aortic vascular endothelial cells. Molecular and Cellular Endocrinology, 399:60-68.
DiVincenzo, L. 2015. DIRECTION OF INDUCED PLURIPOTENT STEM CELL DIFFERENTIATION BY ENDOTHELIAL CELL SECRETOME. Masters Thesis, Kent State University.
Ferrari, L., O. Bogen, P. Green and J. Levine. 2015. Contribution of Piezo2 to endothelium-dependent pain. Molecular Pain, DOI: 10.1186/s12990-015-0068-4.Iaconetti, C., S. De Rosa, A. Polimeni, S. Sorrentino, C. Gareri, A. Carino, J. Sabatino, A. Curcio, and C. Indolfi. 2015. Down-Regulation of miR-23b Induces Phenotypic Switching of Vascular Smooth Muscle Cells in vitro and in vivo. Cardiovascular Research, DOI: http://dx.doi.org/10.1093/cvr/cvv141.
Iaconetti, C., S. De Rosa, A. Polimeni, S. Sorrentino, C. Gareri, A. Carino, J. Sabatino, A. Curcio, and C. Indolfi. 2015. Down-Regulation of miR-23b Induces Phenotypic Switching of Vascular Smooth Muscle Cells in vitro and in vivo. Cardiovascular Research, DOI: http://dx.doi.org/10.1093/cvr/cvv141.
Otto, S., A. Deussen, B. Zatschler, B. Müller, A. Neisser, K. Barth, H. Morawietz and I. Kopaliani. 2015. A Novel Role of Endothelium in Activation of Latent Pro-Membrane Type 1 Matrix Metalloproteinase and Pro-Matrix Metalloproteinase-2 in Rat Aorta. Cardiovascular Research, DOI: http://dx.doi.org/10.1093/cvr/cvv256.
Zhu, S., M. Zhang, J. Davis, W. Wu, K. Surrao, H. Wang and G. Wu. 2015. A single mutation in helix 8 enhances the angiotensin II type 1a receptor transport and signaling. Cellular Signalling, 27:2371-2379.
2014
Ahmed, S., N. Anzures, Z. Bosley, B. Bui, A. Feizi, S. Jawahery, C. Koenig, K. Lakomy, M. Lin, P. Natarajan, E. Nathan, H. Sayed, and E. Solano. 2014. THE APPLICATION OF AN EXOGENOUS LINEAR AND RADIAL ELECTRICAL FIELD TO AN IN VITRO CHRONIC DIABETIC ULCER MODEL FOR EVALUATION AS A POTENTIAL TREATMENT. U Maryland, Digital Repository, http://hdl.handle.net/1903/15531.
Byun, E., N. Sung, M. Yang, D. Song, E. Byun, J. Kim, J. Park, B. Song, J. Lee, S. Park, M. Byun, and J. Kim. 2014. Procyanidin C1 Causes Vasorelaxation Through Activation of the Endothelial NO/cGMP Pathway in Thoracic Aortic Rings. Journal of Medicinal Food, 17:742-748.
Hamid, H., A. Azlina, M. Khamis, R. Rahman, N. Razak, S. Mutum, and A. Samsudin. 2014. Effect of Biphasic Calcium Phosphate Treated with Vascular Endothelial Growth Factor on Osteogenesis and Angiogenesis Gene Expression In Vitro. IFMBE Proceedings, 43:239-242.
Jiang, H., X. Cheng, G. Shi, L. Hu, A. Inoue, Y. Yamamura, H. Wu, K. Takeshita, X. Li, Z. Huang, H., Song, M. Asai, C. Hao, K. Unno, T. Koike, Y. Oshida, K. Okumura, T. Murohara and M. Kuzuya. 2014. Cathepsin K-mediated notch1 activation contributes to neovascularization in response to hypoxia. Nature Comm, 5:3838.
Kopaliani, I. M. Martin, B. Zatschler, K. Bortlik, B. Müller, and A. Deussen. 2014. Cell-specific and endothelium-dependent regulations of matrix metalloproteinase-2 in rat aorta. Basic Research in Cardiology, June, 109:419.
Mears, B., F. Shiao, N. Toney, C. Chen, K. Choi, C. Chow, M. Navin, and H. Shiau. 2014. Salvianolic Acid B Inhibits Growth of Cervical Cancer Cells in vitro via Induction of Apoptosis through the Extrinsic Pathway. U Maryland, Digital Repository.
Twardowski, R. and L. Black. 2014. Cardiac Fibroblasts Support Endothelial Cell Proliferation and Sprout Formation but not the Development of Multicellular Sprouts in a Fibrin Gel Co-Culture Model. Annals of Biomed Eng, 42:1074-1084.2013
Gros, R., Q. Ding, B. Liu, J. Chorazyczewski, and R.D. Feldman. 2013. Aldosterone mediates its rapid effects in vascular endothelial cells through GPER activation. American Journal of Physiology-Cell Physiology. 304:C532-C540.
Nguyen, H., V.L. Chiasson, P. Chatterjee, S.E. Kopriva, K.J. Young, and B.M. Mitchell. 2013. Interleukin-17 causes Rho-kinase-mediated endothelial dysfunction and hypertension. Cardiovascular Research. 97:696-704.
Padilla, J., and N. Jenkins. 2013. Induction of endoplasmic reticulum stress impairs insulin-stimulated vasomotor relaxation in rat aortic rings: role of endothelin-1. J Physiol Pharmacol. 64:557-564.
2012
Byun, E., T. Ishikawa, A. Suyama, M. Kono, S. Nakashima, T. Kanda, T. Miyamoto, and T. Matsui. 2012. A procyanidin trimer, C1, promotes NO production in rat aortic endothelial cells via both hyperpolarization and PI3K/Akt pathways. European Journal of Pharmacology, 692: Issues 1–3, 5 October:52–60.
Byun, E., S. Korematsu, T. Ishikawa, T. Nishizuka, S. Ohshima, T.l Kanda, and T. Matsui. 2012. Apple procyanidins induce hyperpolarization of rat aorta endothelial cells via activation of K+ channels. J Nutional Biochem, 3:278-286.
Liu, B. 2012. The role of GRK2 in hypertension and regulation of GPR30. MS Thesis, University of Western Ontario.
Liu, X., Y. Cheng, J. Yang, L. Xu and C. Zhang. 2012. Cell-specific effects of miR-221/222 in vessels: molecular mechanism and therapeutic application. J Mol Cell Cardiol. 52:245-255. RAOSMC
Masuda, Y., Y. Yamada, and Y. Kimura. 2012. In Vitro Guidance of Dental Pulp Cells by Nd:YAG Laser-Irradiated Endothelial Cells. Photomed Laser Surg, 30:315-319.
2011
Aslam, M., D. Gündüz, D. Schuler, L. Li, F. Sharifpanah, D. Sedding, H.M. Piper, and T. Noll. 2011. Intermedin induces loss of coronary microvascular endothelial barrier via derangement of actin cytoskeleton. Cardiovascular research. 92:276-286.
Chiasson, V.L., M.A. Quinn, K.J. Young, and B.M. Mitchell. 2011. Protein Kinase CβII-Mediated Phosphorylation of Endothelial Nitric Oxide Synthase Threonine 495 Mediates the Endothelial Dysfunction Induced by FK506. J. Pharm.&Exp.Therap.337:718-723.
Duffy, G.P., T.M. McFadden, E.M. Byrne, S.-L. Gill, E. Farrell, and F.J. O’Brien. 2011. Towards in vitro vascularisation of collagen-GAG scaffolds. European cells & materials. 21:15-30.
2010
Ibrahim, S., Q. Kang, and A. Ramamurthi. 2010. The Impact of HA Oligomer Content on Physical, Mechanical, and Biologic Properties of Divinyl Sulfone-Crosslinked HA Hydrogels. J Biomed Mater Res A, 94:355-370.
Imamura, H., T. Ohta, K. Tsunetoshi, K. Doi, K. Nozaki, Y. Takagi, and K.-i. Kikuta. 2010. Transdifferentiation of bone marrow-derived endothelial progenitor cells into the smooth muscle cell lineage mediated by tansforming growth factor-β1. Atherosclerosis. 211:114-121.
Lionetti, V., S. Cantoni, C. Cavallini, F. Bianchi, S. Valente, I. Frascari, E. Olivi, G. Aquaro, F. Bonavita, I. Scarlata, M. Maioli, V. Vaccari, R. Tassinari, A. Bartoli, F. Recchia, G. Pasquinelli, and C. Ventura. 2010. Hyaluronan Mixed Esters of Butyric and Retinoic Acid Affording Myocardial Survival and Repair without Stem Cell Transplantation. JBC, 285:9949-9961.
Mei, H., W. Shi, Z. Pang, H. Wang, W. Lu, X. Jiang, J. Deng, T. Guo and Y. Hu. 2010. EGFP-EGF1 protein-conjugated PEG–PLA nanoparticles for tissue factor targeted drug delivery. Biomaterials, 31:5619-5626.
2008
Hanley, E., J. Lauer, B. Micales, G. Lyons, and J. Shohet. 2008. Surface-directed differentiation of embryonic stem cells. Appl, Phys Lett, 92:193902.
2007
Mattagajasingh, I., C.-S. Kim, A. Naqvi, T. Yamamori, T.A. Hoffman, S.-B. Jung, J. DeRicco, K. Kasuno, and K. Irani. 2007. SIRT1 promotes endothelium-dependent vascular relaxation by activating endothelial nitric oxide synthase. Proceedings of the National Academy of Sciences. 104:14855-14860.
Ohkawara, N., H. Ueda, S. Shinozaki, T. Kitajima, Y. Ito, H. Asaoka, A. Kawakami, E. Kaneko, and K. Shimokada. 2007. Hepatocyte Growth Factor Fusion Protein Having Collagen-Binding Activity (CBD-HGF) Accelerates Re-endothelialization and Intimal Hyperplasia in Balloon-injured Rat Carotid Artery. J Atherosclerosis and Thrombosis, 14:185-191.
2001
Gousseva, N., K. Kugathasan, C.N. Chesterman, and L.M. Khachigian. 2001. Early growth response factor-1 mediates insulin-inducible vascular endothelial cell proliferation and regrowth after injury*. Journal of cellular biochemistry. 81:523-534.