MSDS Cryopreserved Cells
Instructions HLMVEC Normal
5 Important Cell Culture Rules
Cell Apps Flyer Airway Cells
Cell Apps Flyer Cardiovascular Cells
Cell Apps Flyer Endothelial Cells
Cell Apps Poster Primary Cells

Description

Human Lung Microvascular Endothelial Cells (HLMVEC) provide a useful tool for studying various aspects of pathology and biology of the pulmonary microvasculature in vitro. The pulmonary vasculature is of great physiological/pathological significance.  It is now recognized to be a tissue composed of metabolically active, functionally responsive cells, that interact with circulating substrates and regulate the composition of systemic arterial blood, affect target organ functions, and contribute to thrombosis, hemostasis and immune reactions, as well as tumor metastasis. 
 
HLMVEC from Cell Applications, Inc. have been used to elucidate the therapeutic effects of Angiotensin I-converting enzyme (ACE) inhibitors, and the results revealed that they provide an additional benefit to patients by activating bradykinin B1 receptor leading to prolonged nitric oxide (NO) production in endothelial cells and inhibition of PKCϵ.
(Click to Enlarge) Human Lung Microvascular Endothelial Cells (HLMVEC) in culture (A). HLMVEC immunolabeled for CD31/PECAM (green); nuclei are visualized with PI (red) (B).  HLMVEC transfected with GFP plasmid DNA using the Cytofect™ Endothelial Cell Transfection Kit (C,D)

Details

Tissue
Normal healthy human lung
QC
No bacteria, yeast, fungi, mycoplasma, virus
Character
Factor VIII-related Ab, CD31 (PECAM-1), DiI-Ac-LDL uptake
Bioassay
Attach, spread on AFS-coated surface, proliferate in Growth Med
Cryovial
500,000 HLMVEC (3rd passage) frozen in Basal Medium w/ 10% FBS, 10% DMSO
Kit
Cryovial HLMVEC (540-05), Gr Med (111-500), Attchmnt Fctr Soln (123-100), Sbcltr Rgnt Kit (090K)
Proliferating
Shipped in Tsfr Med, 4th psg (flasks or plates)
Doublings
At least 15
Applications
Laboratory research use only (RUO). Not for human, clinical, diagnostic or veterinary use.
Instructions HLMVEC Normal

Format: PDF

Download Now
MSDS Cryopreserved Cells

Format: PDF

Download Now

Products

Product Size CAT.# Price Quantity
Cryopreserved HLMVEC, Adult: 5x10^5 Cells (Adult), Medium & Subculture Reagents (See Details tab for specifics) Size: 1 Kit CAT.#: 540K-05a Price: $944.00
-+
Cryopreserved HLMVEC, Adult: Frozen HLMVEC (5x10^5) Size: 1 Cryovial CAT.#: 540-05a Price: $721.00
-+
Cryopreserved HLMVEC, Pre-Screened, Adult: Frozen HLMVEC (5x10^5) Size: 1 Cryovial CAT.#: S540-05a Price: $869.00
-+
Cryopreserved HLMVEC, Pre-Screened, Adult: 5x10^5 Cells (Pre-Screened, Adult), Medium & Subculture Reagents (See Details tab for specifics) Size: 1 Kit CAT.#: S540K-05a Price: $1,092.00
-+
Cryopreserved HLMVEC-AS, Adult: Frozen HLMVEC-AS from donor with Asthma (5x10^5) Size: 1 Cryovial CAT.#: 540AS-05a Price: $796.00
-+
Cryopreserved HLMVEC-AS, Adult: 5x10^5 Cells (from donor with Asthma, Adult), Medium & Subculture Reagents (See Details tab for specifics) Size: 1 Kit CAT.#: 540ASK-05a Price: $1,019.00
-+
Proliferating HLMVEC, Adult: Actively growing, dividing cells in medium Size: T-25 Flask CAT.#: 541-25a Price: $721.00
-+
Proliferating HLMVEC, Adult: Actively growing, dividing cells in medium Size: T-75 Flask CAT.#: 541-75a Price: $926.00
-+
Proliferating HLMVEC, Adult: Actively growing, dividing cells in medium Size: 6 Well CAT.#: 541-6Wa Price: $926.00
-+
Proliferating HLMVEC, Adult: Actively growing, dividing cells in medium Size: 96 Well CAT.#: 541-96Wa Price: $1,055.00
-+

Related Products

Product Size CAT.# Price Quantity
HMVEC Basal Medium: Basal medium (contains no growth supplement).  Add GS before use. Size: 500 ml CAT.#: 100-500 Price: $57.00
-+
HMVEC Growth Medium: All-in-one ready-to-use Size: 500 ml CAT.#: 111-500 Price: $129.00
-+
HMVEC Growth Medium Kit: Basal medium & growth supplement sold together packaged separately Size: Yields 500 ml CAT.#: 111K-500 Price: $138.00
-+
HMVEC Growth Supplement: Added to Basal Medium to create Growth Medium Size: 30 ml CAT.#: 111-GS Price: $81.00
-+
Human Microvascular Endothelial Cell Growth Medium Kit, separate: Basal medium & growth supplement sold, together packaged separately. Supplements in individual vials. Size: Yields 500 ml CAT.#: 111KS-500 Price: $139.15
-+

Extended Family Products

Product Size CAT.# Price Quantity
Anti-ICAM-1: Rabbit Intercellular Adhesion Molecule-1 Antibody Size: 100 ul CAT.#: CG1238 Price: $275.00
Anti-VEGF: Rabbit Vascular Endothelial Growth Factor Antibody Size: 100 ul CAT.#: CG1634 Price: $275.00
Attachment Factor Solution (AFS): AFS, contains Bovine Gelatin Size: 100 ml CAT.#: 123-100 Price: $19.00
-+
Cyto-X Cell Counting Reagent: 500 tests Size: 1 Bottle CAT.#: 028-01 Price: $139.00
-+
Cyto-X Cell Counting Reagent Sample: 100 tests Size: Sample CAT.#: 028-S Price: $36.00
-+
Cytofect™ Endothelial Cell Transfection Kit: 250 x 24-Well Rxns Size: 1 Kit CAT.#: TF101K Price: $431.00
-+
Cytofect™ Endothelial Cell Transfection Kit: 25 x 24-Well Rxns Size: 1 Sample Kit CAT.#: TF101KS Price: $54.00
-+
Freezing Medium: For general cryopreservation of most primary cells. Contains FBS & DMSO. Size: 50 ml CAT.#: 040-50 Price: $54.00
-+
HLMVEC RNA, Adult: Total RNA prepared from Human Lung Microvascular Endothelial Cells, adult Size: 25 ug CAT.#: 540-R25a Price: $818.00
-+
Human E-Selectin ELISA Kit: Human E-Selectin ELISA Kit Size: 96 Wells CAT.#: CL0501 Price: $587.00
-+
Human Gamma-Interferon Inducible Protein 10 (IP-10 / CXCL10): Human gamma-Interferon Inducible Protein 10 Size: 25 ug CAT.#: RP1127-25 Price: $194.00
-+
Human Gamma-Interferon Inducible Protein 10 (IP-10 / CXCL10): Human gamma-Interferon Inducible Protein 10 Size: 100 ug CAT.#: RP1127-100 Price: $484.00
-+
Human Gamma-Interferon Inducible Protein 10 (IP-10 / CXCL10): Human gamma-Interferon Inducible Protein 10 Size: 1000 ug CAT.#: RP1127-1000 Price: $3,175.00
-+
Human ICAM-1 ELISA Kit: Human Intercellular Adhesion Molecule-1 ELISA Kit Size: 96 Wells CAT.#: CL0370 Price: $484.00
-+
Human Lung RNA: Total RNA prepared from human lung tissue Size: 50 ug CAT.#: 1H40-50 Price: $139.00
-+
Human Lung RNA: Total RNA prepared from human lung tissue Size: 250 ug CAT.#: 1H40-250 Price: $522.00
-+
Human P-Selectin ELISA Kit: Human P-Selectin ELISA Kit Size: 96 wells CAT.#: CL0505 Price: $517.00
-+
Human Vascular Endothelial Growth Factor-121 (VEGF-121): Human Vascular Endothelial Growth Factor-121 Size: 10 ug CAT.#: RP1116-10 Price: $194.00
-+
Human Vascular Endothelial Growth Factor-121 (VEGF-121): Human Vascular Endothelial Growth Factor-121 Size: 100 ug CAT.#: RP1116-100 Price: $484.00
-+
Human Vascular Endothelial Growth Factor-121 (VEGF-121): Human Vascular Endothelial Growth Factor-121 Size: 1000 ug CAT.#: RP1116-1000 Price: $4,090.00
-+
Human VEGF-121, Animal-Free: Human Vascular Endothelial Growth Factor-121, Animal-Free Size: 10 ug CAT.#: RP1116AF-10 Price: $213.00
-+
Human VEGF-121, Animal-Free: Human Vascular Endothelial Growth Factor-121, Animal-Free Size: 100 ug CAT.#: RP1116AF-100 Price: $533.00
-+
Human VEGF-121, Animal-Free: Human Vascular Endothelial Growth Factor-121, Animal-Free Size: 1000 ug CAT.#: RP1116AF-1000 Price: $4,499.00
-+
Human VEGF-c ELISA Kit: Human Vascular Endothelial Growth Factor C ELISA Kit Size: 96 Wells CAT.#: CL0588 Price: $581.00
-+
Polyclonal Vascular Endothelial Growth Factor-C Antibody: Polyclonal Vascular Endothelial Growth Factor-C Antibody Size: 100 ul CAT.#: CB3778 Price: $302.00
-+
Polyclonal VEGF Receptor 1 Antibody: Polyclonal VEGF Receptor 1 Antibody Size: 100 ul CAT.#: CB3839 Price: $333.00
-+
Subculture Reagent Kit: 100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution Size: 1 Kit CAT.#: 090K Price: $55.00
-+

Resources/Documents

5 Important Cell Culture Rules

Format: PDF

Downoad Now
Cell Apps Flyer Airway Cells

Format: PDF

Downoad Now
Cell Apps Flyer Cardiovascular Cells

Format: PDF

Downoad Now
Cell Apps Flyer Endothelial Cells

Format: PDF

Downoad Now
Cell Apps Poster Primary Cells

Format: PDF

Downoad Now

Publications

2015
Bai, L., H. Andersson, K. McConnell, M. Hernandez, J. Gonzalez, X. Liu, S. La Francesca, J. Sakamoto and R. Serda. 2015. Silencing of Tumor Necrosis Factor Receptor-1 in Human Lung Microvascular Endothelial Cells Using Particle Platforms for siRNA Delivery. Current Drug Targets, 16:1531-1539.
Li, C., C. Gonsalves, M. Eiymo Mwa Mpollo, P. Malik, S. Tahara, and V. Kalra. 2015. MicroRNA 648 Targets ET-1 mRNA and Is Cotranscriptionally Regulated with MICAL3 by PAX5. Molecular and Cellular Biology, 35:514-528.
2014
Li, C., M. Mpollo, C. Gonsalves, S. Tahara, P. Malik and V. Kalra. 2014. Peroxisome Proliferator-activated Receptor-α-mediated Transcription of miR-199a2 Attenuates Endothelin-1 Expression via Hypoxia-inducible Factor-1α.  J Biol Chem, 289:36031-36047.
2006
Stanisavljevic, S., T. Ignjatovic, P.A. Deddish, V. Brovkovych, K. Zhang, E.G. Erdös, and R.A. Skidgel. 2006. Angiotensin I-Converting Enzyme Inhibitors Block Protein Kinase Cϵ by Activating Bradykinin B1 Receptors in Human Endothelial Cells. Journal of Pharmacology and Experimental Therapeutics. 316:1153-1158
2004
Ignjatovic, T., S. Stanisavljevic, V. Brovkovych, R.A. Skidgel, and E.G. Erdos. 2004. Kinin B1 Receptors Stimulate Nitric Oxide Production in Endothelial Cells: Signaling Pathways Activated by Angiotensin I-Converting Enzyme Inhibitors and Peptide Ligands. Molecular Pharmacology. 66:1310-1316.