Porcine Pulmonary Artery Endothelial Cells: PPAEC
Porcine Pulmonary Artery Endothelial Cells: PPAEC
Porcine Pulmonary Artery Endothelial Cells (PPAEC) are primary endothelial cells derived from the pulmonary vasculature of pigs.
Description
Porcine Pulmonary Artery Endothelial Cells (PPAEC) are primary endothelial cells derived from the pulmonary vasculature of pigs, serving as a well-established model in vascular biology and cardiovascular research.
In vivo, pulmonary artery endothelial cells line the inner surface of major blood vessels, where they regulate vascular tone, barrier permeability, and local hemodynamic responses. In experimental studies, PPAEC have been used to investigate cellular signaling pathways related to endothelial dysfunction and vascular disease mechanisms. For instance, researchers have utilized PPAEC to examine how asymmetric dimethylarginine (ADMA) — an endogenous inhibitor of nitric oxide production and a recognized cardiovascular risk factor — can modulate protein kinase G (PKG)–mediated signaling, cytoskeletal organization, and endothelial barrier permeability. Furthermore, PPAEC have been applied in translational investigations to assess endothelial adhesion and biocompatibility on decellularized porcine arteries, supporting efforts to develop engineered vascular grafts for human vascular repair.
In broader biomedical and veterinary research, PPAEC and related animal cells or cell lines provide valuable experimental platforms for studying cellular processes under controlled in vitro conditions. While these animal-derived models allow investigators to evaluate cellular responses, gene expression profiles, proliferation dynamics, and molecular interactions using techniques such as flow cytometry, findings from porcine endothelial studies are often paired with complementary data from human systems and clinical samples to advance our understanding of vascular diseases, atherosclerosis, and tissue regeneration. Maintaining experimental control and cellular integrity involves careful handling protocols, including storage in liquid nitrogen and rigorous monitoring to ensure reproducible results across various experimental applications.
Porcine Pulmonary Artery Endothelial Cells (PPAEC) from Cell Applications, Inc. have been used to:
- Determine that asymmetric dimethylarginine (ADMA), an inhibitor of nitric oxide production and a cardiovascular risk factor, decreased PKG-mediated phosphorylation of VASP
- This event led to downregulation of Rac1, and induction of stress fibers & focal adhesions via a RhoA/ROCK dependent mechanism
- The resulting decreased cell motility, and increase in endothelial permeability, linked vascular repair and function with NO production
- PPAEC have also been used to assess endothelial adhesion to decellularized porcine arteries in order to develop grafts for human vascular repair
Details
| Tissue | Normal healthy porcine pulmonary artery | |
|---|---|---|
| QC | No bacteria, yeast, fungi, mycoplasma | |
| Character | DiI-Ac-LDL uptake: Positive | |
| Bioassay | Attach, spread, proliferate in Growth Med | |
| Cryovial | 500,000 PPAEC (1st passage) frozen in Basal Medium w/ 10% FBS, 10% DMSO | |
| Kit | Cryovial frozen PPAEC (P302-05), Growth Medium (P211-500), Subculture Rgnt Kit (090K) | |
| Proliferating | Shipped in Gr Med, 2nd psg (flasks or plates) | |
| Doublings | At least 16 | |
| Applications | Laboratory research use only (RUO). Not for human, clinical, diagnostic or veterinary use. |
Resources
FAQs
Need More Help?
Visit our comprehensive FAQ page for detailed answers to common questions.
Need More Help?
Visit our comprehensive FAQ page for detailed answers to common questions.
Primary Cell FAQs
Powered by Bioz