Instructions 017DK i-HCm Differentiation Medium Kit
From Skin Cells to Beating Heart Cells Dermal fibroblasts (HDF) isolated from human skin, reprogrammed to pluripotent stem cells (HiPSC), then differentiated to cardiomyocytes (i-HCm). (a) In vitro-cultured i-HCm immunofluorescently stained for Alpha Actinin (b) Troponin, and nuclei counter-stained for DAPI. (c) Pulsating heart tissue spheroid (organoid) constructed with Cyfuse Regenova Bio 3D Printer, incorporating (left) neonatal or (right) adult i-HCm & primary cardiac cells. Printed i-HCm derived from adult iPSC exhibit strong beating at 72 pulses per minute while neonatal i-HCm demonstrate weaker, faster beat rate. (d): Monolayer-cultured i-HCm beat spontaneously in rhythm.
Medium carefully optimized for differentiating Human Induced Pluripotent Stem Cells into iPSC-Derived Cardiomyocytes and maintaining i-HCm. Attention to detail ensures ideal cell health, viability, performance, physiology, morphology, consistency and data.
Use with: Human Induced Pluripotent Stem Cells
HiPSC to i-HCm timeline using Differentiation Medium Kit (017DK)
i-HCm Differentiation Medium Kit, Xeno-Free (017DK)
|017D1-30||Differentiation 1||i-HCm DM1||30ml|
|017D2-30||Differentiation 2||i-HCm DM2||30ml|
|017D3-100||Differentiation 3||i-HCm DM3||100ml|
Store all kit components at 4°C. Stable for 4 weeks at 4ºC.
|Human Cardiomyocyte Differentiation Medium: Differentiation, Selection & Maintenance Media sold together, packaged separately. See Details Tab.||Size: 1 Kit||CAT.#: 017DK||Price: $250.00|
|Human Cardiomyocyte Maintenance Medium: Maintenance medium for Human iPSC-Derived Cariomyocytes||Size: 50 ml||CAT.#: 021-50||Price: $59.00|
|Human Cardiomyoycte Plating Medium: Use for initial plating of Human iPSC-Derived Cardiomyocytes||Size: 20 ml||CAT.#: 021P-20||Price: $59.00|
Extended Family Products
|Cryopreserved HiPSC, from Adult Female HDF: Frozen Human Induced Pluripotent Stem Cells derived from HDF (1x10^6) (Donor age, gender in Details Tab)||Size: 1 Cryovial||CAT.#: iPS12-10||Price: $692.00|
|Cryopreserved HiPSC, from Neonatal Male HDF: Frozen Human Induced Pluripotent Stem Cells derived from HDF (1x10^6) (Donor age, gender in details)||Size: 1 Cryovial||CAT.#: iPS11-10||Price: $692.00|