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Subculture Reagents

Subculture Reagents: Trypsin/EDTA, HBSS, Trypsin Neutralizing Solution formulated to keep cells healthy during cell passage.  Optimized for effectiveness, population doublings and cell viability.
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For optimal cell propagation, researchers depend on CAI Trypsin/EDTA, Trypsin Neutralization Solution, HBSS or all three together in convenient Subculture Reagent Kits. Hank’s Balanced Salt Solution is used to wash cells, Trypsin/EDTA removes adherent cells from the culture surface, and Trypsin Neutralization Solution halts the process. Our Cell Lysis buffer breaks down cell membranes to yield lysate containing protein, DNA, RNA, and for further purification. 
 
 
Need help choosing?  See our Media Selection Guide.
2017
Bidarimath, M., K. Khalaj, R. Kridli, F. Kan, M. Koti, and C. Tayade. 2017. Extracellular vesicle mediated intercellular communication at the porcine maternal-fetal interface: A new paradigm for conceptus-endometrial cross-talk. Scientific Reports, doi:10.1038/srep40476.
2016
Bidarimath, M. 2016. Immune-Angiogenesis Mechanisms Associated with Porcine Pregnancy Success and Failure. PhD Thesis, Queen’s University.
Li, H., J. Qian, J. Chen, K. Zhong and S. Chen. 2016. Osteochondral repair with synovial membrane‑derived mesenchymal stem cells. Molecular Medicine Reports, 13:2071-2077.
Ma, L., X. Xun, Y. Qiao, J. An and M. Su. 2016. Predicting efficacies of anticancer drugs using single cell HaloChip assayAnalyst, 141:2454-2462.
Raghavendran, H., S. Mohan, K. Genasan, M. Murali, S. Naveen, S. Talebian, R. McKean and T. Kamarul. 2016. Synergistic interaction of platelet derived growth factor (PDGF) with the surface of PLLA/Col/HA and PLLA/HA scaffolds produces rapid osteogenic differentiation. Colloids and Surfaces B: Biointerfaces, 139:68-78.
Zeimaran, E., S. Mohan, S. Pourshahrestani, B. Pingguan-Murphy, N. Kadri, M. Murali, H. Raghavendran, K. Hasikin, T. Kamarul and M. Towler. 2016. Osteogenic differentiation of mesenchymal stem cells on a poly (octanediol citrate)/bioglass composite scaffold in vitro. Materials & Design, 109:434-442.
2015
de Sá Rodrigues, L., K. Holmes, V. Thompson, M. Newton and T. Stein. 2015. Tumourigenic canine osteosarcoma cell lines associated with frizzled-6 up-regulation and enhanced side population cell frequencyVet & Comp Oncol, DOI: 10.1111/vco.12138. SC 
González-Miguel, J., R. Morchón, E. Carretón, J. Montoya-Alonso and F. Simón. 2015. Can the activation of plasminogen/plasmin system of the host by metabolic products of Dirofilaria immitis participate in heartworm disease endarteritisParasites & Vectors, 8:194.
Puvaneswary, S., S. Talebian, H. Raghavendran, M. Murali, M. Mehrali, A. Afifi, N. Kasim and T. Kamarul. 2015. Fabrication and in vitro biological activity of βTCP-Chitosan-Fucoidan composite for bone tissue engineeringCarbohydrate Polymers, 134:799–807. 
2014
Pun, P., A. Logan, V. Darley-Usmar, B. Chacko, M. Johnson, G. Huang, S. Rogatti, T. Prime, C. Methner, T. Krieg, I. Fearnley, L. Larsen, D. Larsen, K. Menger, Y. Collins, A. James, G. Kumar, R. Hartley, R. Smith and M. Murphy. 2014. A mitochondria-targeted mass spectrometry probe to detect glyoxals: implications for diabetes. Free Rad Biol & Med, 67:437-450.
Shu, B., J. Xie, Y. Xu, W. Lai, Y. Huang, R. Mao, X. Liu and S Qi. 2014. Effects of skin-derived precursors on wound healing of denervated skin in a nude mouse modelInt J Clin Exp Pathol, 8:2660-2669.
Talebian, S., M. Mehrali, S. Mohan, H. Raghavendran, M. Mehrali, H. Khanlou, T. Kamarul, A. Afifi and A. Abass. 2014. Chitosan (PEO)/bioactive glass hybrid nanofibers for bone tissue engineering. RSC Adv, 4:49144-49152.
Raghavendran, H., S. Puvaneswary, S. Talebian, M. Murali, S. Naveen, G. Krishnamurithy, R. McKean and T. Karmarul. 2014. A Comparative Study on In Vitro Osteogenic Priming Potential of Electron Spun Scaffold PLLA/HA/Col, PLLA/HA, and PLLA/Col for Tissue Engineering Application.
PLoS ONE, dx.doi.org/10.1371/journal.pone.0104389.
2012
Ohta, K. 2012. Method for producing pluripotent cell using bacterium having fermentation ability. Patent Aplication US 20140255942 A1.
Jin, M., A. Wu, S. Dorzhin, Q. Yue, Y. Ma and D. Liu. 2012. Culture conditions for bovine embryonic stem cell-like cells isolated from blastocysts after external fertilization. Cytotechnology, 64:379-389.  “Bovine Embryonic Fibroblast GM”??
Liu, D., Y. Lin, T. Kang, B. Huang, W. Xu, M. Garcia-Barrio, M. Olatinwo, R. Mattews, Y. Chen and W. Thompson. 2012. Mitochondrial Dysfunction and Adipogenic Reduction by Prohibitin Silencing in 3T3-L1 Cells. PLoS ONE, dx.doi.org/10.1371/journal.pone.0034315.
Villareal, M., J. Han, K. Ikuta, and H. Isoda. 2012. Mechanism of Mitf inhibition and morphological differentiation effects of hirsein A on B16 melanoma cells revealed by DNA microarray. J Dermatol Sci, 67:26-36. 
2011
Nguyen, L., S. Liao, S. Ramakrishna, and C. Chan. 2011. The role of nanofibrous structure in osteogenic differentiation of human mesenchymal stem cells with serial passage. Nanomedicine, 6:961-974.  
Yang, C.J., C.Y. Lin, T.-c. Hsieh, S.C. Olson, and J.M. Wu. 2011. Control of eotaxin-1 expression and release by resveratrol and its metabolites in culture human pulmonary artery endothelial cells. American journal of cardiovascular disease. 1:16.
2010
Wakabayashi, M., Y. Ito, T. Hamazaki and H. Okochi. 2010. Efficient Myogenic Differentiation of Murine Dermal Sca-1 (−) Cells via Initial Aggregation Culture. Tiss Eng Pt A, 16:3251-3259.
2009
Ilagan, B.G., and B.G. Amsden. 2009. Surface modifications of photocrosslinked biodegradable elastomers and their influence on smooth muscle cell adhesion and proliferation. Acta Biomaterialia. 5:2429-2440.
 

Products

Hank's Balanced Salt Solution

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Description
Size
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Hank's Balanced Salt Solution 1X HBSS for washing of cells before Trypsin/EDTA treatment 100 ml 062-100 $18.30

Lysis Buffer

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Lysis Buffer For mammalian cell lysis. 5 ml 030-005 $21.53

Subculture Reagent Kit

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Subculture Reagent Kit 100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution 1 Kit 090K $54.89

Trypsin Neutralization Solution

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Description
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Trypsin Neutralization Solution Stops the trypsinization reaction. 100 ml 080-100 $18.30

Trypsin/EDTA Solution

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Description
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Trypsin/EDTA Solution For subculturing of adherent cells. 100 ml 070-100 $18.30

Extended Family Products

Cyto-X Colorimetric Cell Counting Reagent

Pricing
Description
Size
Cat. #
Price
Cyto-X Colorimetric Cell Counting Reagent 500 tests 1 Bottle 028-01 $138.84
Cyto-X Colorimetric Cell Counting Reagent 100 tests Sample 028-S $35.88