Instructions RHbN
MSDS Cryopreserved Cells
5 Important Cell Culture Rules
Cell Apps Flyer Nervous System
Cell Apps Flyer Brain Cells
Cell Apps Poster Primary Cells
Cell Applications Inc Brochure

Description

Rat Hindbrain Neurons (RHbN) are derived from hindbrains of normal embryonic rat (gestation day 15) by standardized methods. When cultured under the recommended conditions, RHbN arborize and form complex neurite network in one week. RHbN Stain positive for β III-Tubulin.  RHbN are available in multiple formats, including cryopreserved neurons, freshly isolated primary neurons in suspension, or pre-plated cultured neurons.

The hindbrain includes the cerebellum, the pons, and the medulla. These parts together control and support various vital bodily processes such as breathing, swallowing, blood circulation, muscle tone, sleep and arousal patterns, coordination, equilibrium maintenance, and more. Malformation, vascular disorders, atrophies, and traumas at hindbrain strike its proper structure and functions, leading to the manifestation of a variety of clinical features of different severity.  Studying hindbrain neurons in those disease conditions will allow a better understanding of the disease mechanisms, physiopathologies, and advancement in drug development and therapies. Cultures of hindbrain neurons can be applied for a variety of experiments including toxicity test, immunostaining, live cell imaging, co-culturing, electrophysiology, and more.

(Click to Enlarge) Cryopreserved Rat Hindbrain Neurons (RHbN) revived and cultured for 8 days (top), stained (bottom) for β III-Tubulin (green), GFAP (red), and DAPI (blue).

Details

TissueNormal healthy rat hindbrain
QCNo bacteria, yeast, fungi, mycoplasma, virus  
CharacterPositive for β-III Tubulin
BioassayPlate on Poly-D-Lysine coated surface, arborize to form neurite network in Culture Med
Cryovial1,000,000 RHbN in Ser-Fr Frzng Med (042-50)
Fresh CultureContact CAI if interested in freshly isolated RHbN (suspension or plated)
KitCryovial frozen RHbN, Ctng Soln I (027-05), Plating Med (R817P-10), Cultr Med (R817-100)
DoublingsN/A - Neurons don't proliferate in vitro
ApplicationsLaboratory research use only (RUO). Not for human, clinical, diagnostic or veterinary use.
Instructions RHbN

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MSDS Cryopreserved Cells

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Products

Product Size CAT.# Price Quantity
Cryopreserved RHbN: Frozen RHbN (1x10^6) Size: 1 Cryovial CAT.#: R888N-10 Price: $446.00
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Cryopreserved RHbN : 1x10^6 Cells, Plating Medium, Culture Medium & Coating Solution (See Details tab for specifics) Size: 1 Kit CAT.#: R888NK-10 Price: $535.00
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Freshly isolated RHbN: Contact CAI for details Size: 1 Tube CAT.#: R889NF-10 Price: $0.00
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Pre-plated freshly isolated RHbN: Contact CAI for details Size: 6 Well CAT.#: R889NF-6W Price: $0.00
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Pre-plated freshly isolated RHbN: Contact CAI for details Size: 24 Well CAT.#: R889NF-24W Price: $0.00
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Pre-plated freshly isolated RHbN: Contact CAI for details Size: 96 Well CAT.#: R889NF-96W Price: $0.00
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Related Products

Product Size CAT.# Price Quantity
Neuron Coating Solution I: Poly-D-Lysine coating for neurons Size: 5 ml CAT.#: 027-05 Price: $16.00
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Neuron Coating Solution I: Poly-D-Lysine coating for neurons Size: 10 ml CAT.#: 027-10 Price: $31.00
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Rat Neuron Basal Medium: Basal medium (contains no growth supplement).  Add GS before use. Size: 100 ml CAT.#: R816-100 Price: $31.00
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Rat Neuron Culture Medium: For the culture and maintenance of neurons. Size: 100 ml CAT.#: R817-100 Price: $63.00
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Rat Neuron Culture Medium Kit: Basal medium & growth supplement sold together packaged separately Size: Yields 100 ml CAT.#: R817K-100 Price: $72.00
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Rat Neuron Culture Supplement: Added to Basal Medium to create Culture Medium Size: 3 ml CAT.#: R817-GS Price: $57.00
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Rat Neuron Plating Solution I: For initial plating of neurons Size: 10 ml CAT.#: R817P-10 Price: $20.00
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Rat Neuron Plating Solution I: For initial plating of neurons Size: 20 ml CAT.#: R817P-20 Price: $39.00
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Rat Neuron Plating Solution I: For initial plating of neurons Size: 40 ml CAT.#: R817P-40 Price: $62.00
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Extended Family Products

Product Size CAT.# Price Quantity
Cyto-X Cell Counting Reagent: 500 tests Size: 1 Bottle CAT.#: 028-01 Price: $139.00
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Cyto-X Cell Counting Reagent Sample: 100 tests Size: Sample CAT.#: 028-S Price: $36.00
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Cytofect™ Neuron Transfection Kit: 100 x 24-Well Rxns Size: 1 Kit CAT.#: TF886K Price: $404.00
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Cytofect™ Neuron Transfection Kit: 25 x 24-Well Rxns Size: 1 Sample Kit CAT.#: TF886KS Price: $54.00
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Rat Neuron Culture Medium: For the culture and maintenance of neurons Size: 150 ml CAT.#: R817-150 Price: $92.00
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Resources/Documents

5 Important Cell Culture Rules

Format: PDF

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Cell Apps Flyer Nervous System

Format: PDF

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Cell Apps Flyer Brain Cells

Format: PDF

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Cell Apps Poster Primary Cells

Format: PDF

Downoad Now
Cell Applications Inc Brochure

Format: PDF

Downoad Now