Instructions RDRGN
5 Important Cell Culture Rules
MSDS Cryopreserved Cells
Cell Apps Flyer Nervous System
Cell Apps Flyer Brain Cells
Cell Apps Poster Primary Cells
Cell Applications Inc Brochure

Description

Lying along the vertebral column by the spine, Rat Dorsal Root Ganglion Neurons (RDRGN) are nerve cell body clusters containing afferent neurons that relay sensory information from the peripheral body to the CNS. They are used to study ion channel properties, modulation, neuropathy, peripheral nerve injury, regeneration, myelination, synaptogenesis & more.
Figure: Superior Growth of Neurons Cultured in Cell Applications Medium versus Competitor. 
Notably, Rat Dorsal Root Ganglion Neurons exhibit superior viability and neurite extensions when cultured in optimized media from Cell Applications, compared to a leading competitor medium. In the above Figure, RDRGN (Cat # R8820N-05) were cultured at a density of at 1x10^5 cells/cm2 in  Rat Ganglion Neuron Culture Medium from Cell Applications, Inc. (Top) or competitor Neuron Medium + 100ng/ml rNGF + mitotic inhibitors (Bottom).  Microscopic images were captured at days 1, 2 & 7, clearly showing optimal growth in the CAI medium.
High quality Rat Dorsal Root Ganglion Neurons (RDRGN) are derived from spinal cords of normal embryonic rat (gestation day 15) by standardized methods. When cultured under the recommended conditions with specialty culture medium, cell death occurs during the first few days after plating. The remaining pure RDRGN arborize and form complex neurite network in one week. RDRGN Stain positive for β III-Tubulin.
(Click to Enlarge) Cryopreserved Rat Dorsal Root Ganglion Neurons (RDRGN) revived and cultured for 6 days (top), stained (bottom) on day 14 for β III-Tubulin (green), S100β (red), and DAPI (blue).

Details

TissueNormal healthy rat spinal cord
QCNo bacteria, yeast, fungi, mycoplasma
CharacterPositive for β-III Tubulin
BioassayCells plate on Neuron Coating Solution II-coated surface, Arborize to form neurite network in Culture Medium
Cryovial500,000 RDRGN in Ser-Fr Frzng Med (042-50)
Fresh CultureContact CAI if interested in freshly isolated RDRGDN (suspension or plated)
KitCryovial RDRGN, Ctng Soln II (029-05), Pltng Med (R823P-10), Cultr Med Kit (R823K-50)
DoublingsN/A - Neurons don't proliferate in vitro
ApplicationsLaboratory research use only (RUO). Not for human, clinical, diagnostic or veterinary use.
Instructions RDRGN

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MSDS Cryopreserved Cells

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Products

Product Size CAT.# Price Quantity
Cryopreserved RDRGN: Frozen RDRGN (5x10^5) Size: 1 Cryovial CAT.#: R8820N-05 Price: $353.63
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Cryopreserved RDRGN: Frozen RDRGN (1x10^6) Size: 1 Cryovial CAT.#: R8820N-10 Price: $640.63
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Cryopreserved RDRGN Total Kit: 5x10^5 Cells, Plating Medium, Culture Medium & Coating Solution (See Details tab for specifics) Size: 1 Kit CAT.#: R8820NK-05 Price: $515.83
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Freshly isolated RDRGN: Contact CAI for details Size: 1 Tube CAT.#: R8821NF-05 Price: $0.00
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Pre-plated freshly isolated RDRGN: Contact CAI for details Size: 6 Well CAT.#: R8821NF-6W Price: $0.00
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Pre-plated freshly isolated RDRGN: Contact CAI for details Size: 24 Well CAT.#: R8821NF-24W Price: $0.00
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Pre-plated freshly isolated RDRGN: Contact CAI for details Size: 96 Well CAT.#: R8821NF-96W Price: $0.00
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Related Products

Product Size CAT.# Price Quantity
Neuron Coating Solution II: Poly-D-Lysine coating for neurons, especially RDGRN and HBN. Size: 5 ml CAT.#: 029-05 Price: $26.91
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Rat Ganglion Neuron Culture Medium Kit: Basal Medium and 5 Supplements bottled separately Size: Yields 100 ml CAT.#: R823K-100 Price: $128.07
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Rat Ganglion Neuron Plating Medium: Use for initial plating of ganglion neurons. Size: 10 ml CAT.#: R823P-10 Price: $20.45
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Rat Neuron Culture Supplement: Added to Basal Medium to create Culture Medium Size: 3 ml CAT.#: R817-GS Price: $57.04
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Extended Family Products

Product Size CAT.# Price Quantity
Cyto-X Colorimetric Cell Counting Reagent: 500 tests Size: 1 Bottle CAT.#: 028-01 Price: $138.84
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Cyto-X Colorimetric Cell Counting Reagent: 100 tests Size: Sample CAT.#: 028-S Price: $35.88
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Cytofect™ Neuron Transfection Kit: 100 x 24-Well Rxns Size: 1 Kit CAT.#: TF886K Price: $403.59
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Cytofect™ Neuron Transfection Kit: 25 x 24-Well Rxns Size: 1 Sample Kit CAT.#: TF886KS Price: $53.76
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Resources/Documents

5 Important Cell Culture Rules

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Cell Apps Flyer Nervous System

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Cell Apps Flyer Brain Cells

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Cell Apps Poster Primary Cells

Format: PDF

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Cell Applications Inc Brochure

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