Description
Cell Applications' newly released primary Human Neural Stem Cells (HNSC) offer a robust, adherent 2D culture system ideal for research in neurodevelopment, neurotoxicity, and cell therapy modeling. Available in both cryovials and actively proliferating formats (flasks or multiwell plates), these HNSC are derived from brain samples, are proliferative and multipotent.
HNSC are cultured using Cell Applications' novel, proprietary Neural Longevity Matrix (NLM), which supports sustained proliferation as an adherent culture across multiple passages without loss of stemness. Upon induction, HNSC can be efficiently differentiated into neurons, astrocytes, and oligodendrocytes using lineage-specific differentiation media (Cat# 813D-100 Neuron Differentiation Media, Cat# 813D-100A Astrocyte DM, and Cat# 813D-100O Oligodendrocyte DM). Differentiation is confirmed by immunostaining for key markers: β-tubulin III (neurons), GFAP (astrocytes), and O4 (oligodendrocytes). This system provides a physiologically relevant, scalable platform for CNS modeling and translational neuroscience research.
Cells differentated from HNSC-2D stain positive for lineage-specific markers after the HNSC-2D are cultured on NLM and treated witih lineage specific differentiation mediu. Cell Applications HNSC-2D differentiated in Neuron Differentiation Medium for 3 weeks stain positive for neural marker β-tubulin III. Cell Applications , Cell Applications HNSC-2D differentiated in Astrocyte Differentiation Medium for 10 days stain positive for astrocyte marker GFAP. Cell Applications HNSC-2D differentiated in Oligodendocyte Differentiation Medium for 4 weeks stain positive for oligodendrocyte marker O4.
Details
Tissue | Normal healthy human brain cortex |
QC | No bacteria, yeast, fungi, mycoplasma, virus |
Cryovial | 1,000,000 HNSC (1st psg) cryopreserved in Freezing Med ( ) |
Kit | Cryovial frozen HNSC-2D (HS8836-10f), Growth Medium Kit (813K-2D-250), Neural Longevity Matrix (1041K-2), ROCK Inhibitor, Dissociation Solution (076-20), Non-Stick Solution (1025-05). |
Proliferating | |
Doublings | |
Applications | Laboratory research use only (RUO). Not for human, clinical, diagnostic or veterinary use. |
Products
| Product | Size | CAT.# | Price | Quantity |
|---|---|---|---|---|
| Cryopreserved Human Neural Stem Cells (2D, Adherent) Total Kit: 1x10^6 Cells, Medium, Neural Longevity Matrix, Inhibitor, Dissociation Solution, and Non-Stock Solution | Size: 1 Kit | CAT.#: HS8836K-10f | Price: $1,381.00 | |
| Cryopreserved Neural Stem Cells (HNSC-2D): Frozen Human Neural Stem Cells (1x10^6) | Size: 1 Cryovial | CAT.#: HS8836-10f | Price: $985.00 |
Related Products
| Product | Size | CAT.# | Price | Quantity |
|---|---|---|---|---|
| HNSC-2D Growth Medium Kit: Basal medium & growth supplement sold together packaged separately. | Size: Yields 250 ml | CAT.#: 813K-2D-250 | Price: $198.00 | |
| Neural Longevity Matrix (NLM): Coating kit for 2D cell culture | Size: Yields 2 ml | CAT.#: 1041K-2 | Price: $125.00 | |
| DISCUS Non-Stick Solution: Reagent for cell culture. | Size: 5 ml | CAT.#: 1025-05 | Price: $35.00 |
Extended Family Products
| Product | Size | CAT.# | Price | Quantity |
|---|---|---|---|---|
| HNSC Differentiation Medium, Neuron Lineage: Differentiates HNSC to Neurons | Size: 100 ml | CAT.#: 813D-100N | Price: $88.00 | |
| HNSC Differentiation Medium, Astrocyte Lineage: Differentiates HNSC to Astrocytes | Size: 100 ml | CAT.#: 813D-100A | Price: $73.00 | |
| HNSC Differentiation Medium, Oligodendrocyte Lineage: Differentiates HNSC to Oligodendrocytes | Size: 100 ml | CAT.#: 813D-100O | Price: $73.00 |