Description
Cell Applications' newly released primary adherent Human Neural Stem Cells (HNSC-2D) offer a robust, adherent 2-dimensional culture system ideal for research in neurodevelopment, neurotoxicity, and cell therapy modeling. Available in both cryovials and actively proliferating formats (flasks or multiwell plates), these HNSC are derived from brain samples and multipotent.
HNSC-2D are cultured using Cell Applications' novel, proprietary Neural Longevity Matrix (NLM, Cat# 1041K-2), which supports sustained proliferation as an adherent culture across multiple population doublings without loss of stemness.
To differentiate HNSC-2D, the cells are seeded using NSC Differentiation Coating Solutions A & B (Cat# 034-10 & 035-10), and can be efficiently differentiated into neurons, astrocytes, and oligodendrocytes using lineage-specific differentiation media (Cat# 813D-100 Neuron Differentiation Media, Cat# 813D-100A Astrocyte DM, and Cat# 813D-100O Oligodendrocyte DM). Differentiation is confirmed by immunostaining for key markers: β-tubulin III (neurons), GFAP (astrocytes), and O4 (oligodendrocytes). This system provides a physiologically relevant, scalable platform for CNS modeling and translational neuroscience research.
Top Left: HNSC-2D.
Cell Applications HNSC-2D cultured on NLM and treated witih the following lineage-specific Differentiation Medium (DM) result in expression of lineage-specific markers:
Top Right: HNSC-2D differentiated in Neuron Differentiation Medium for 3 weeks stain positive for neural marker β-tubulin III.
Bottom Left: HNSC-2D differentiated in Astrocyte Differentiation Medium for 10 days stain positive for astrocyte marker GFAP.
Bottom Right: HNSC-2D differentiated in Oligodendocyte Differentiation Medium for 4 weeks stain positive for oligodendrocyte marker O4.
Details
Tissue | Normal healthy human brain cortex |
QC | No bacteria, yeast, fungi, mycoplasma, virus |
Cryovial | 1,000,000 HNSC (1st psg) cryopreserved in Freezing Med ( ) |
Kit | Cryovial frozen HNSC-2D (HS8836-10f), Growth Medium Kit (813K-2D-250), Neural Longevity Matrix (1041K-2), ROCK Inhibitor, Dissociation Solution (076-20), Non-Stick Solution (1025-05). |
Cultured | Shipped in flasks or plates in medium. |
Doublings | At least 8 |
Applications | Laboratory research use only (RUO). Not for human, clinical, diagnostic or veterinary use. |
Products
| Product | Size | CAT.# | Price | Quantity |
|---|---|---|---|---|
| Cryopreserved Human Neural Stem Cells (HNSC-2D, Adherent) Total Kit: 1x10^6 Cells, Medium, Neural Longevity Matrix, Inhibitor, Dissociation Solution, and Non-Stock Solution | Size: 1 Kit | CAT.#: HS8836K-10f | Price: $1,381.00 | |
| Cryopreserved Human Neural Stem Cells (HNSC-2D, Adherent): Frozen Human Neural Stem Cells (1x10^6) | Size: 1 Cryovial | CAT.#: HS8836-10f | Price: $985.00 |
Related Products
| Product | Size | CAT.# | Price | Quantity |
|---|---|---|---|---|
| HNSC-2D Growth Medium Kit: Basal medium & growth supplement sold together packaged separately. | Size: Yields 250 ml | CAT.#: 813K-2D-250 | Price: $198.00 | |
| Neural Longevity Matrix (NLM): Specially formulated proprietary coating colution kit for 2D culture of neural cells. For differentiation of NSC to neural lineages, use NSC Differentiation Coating Solutions A & B. | Size: Yields 2 ml | CAT.#: 1041K-2 | Price: $125.00 | |
| DISCUS Non-Stick Solution: Reagent for cell culture. | Size: 5 ml | CAT.#: 1025-05 | Price: $35.00 | |
| Neural Stem Cell Differentiation Coating Solution A: Coating Solution A used specifically for the differentiation of neural stem cells, such as HNSC and i-HNSC. | Size: 10 ml | CAT.#: 034-10 | Price: $58.00 | |
| Neural Stem Cell Differentiation Coating Solution B: Coating Solution B used specifically for the differentiation of neural stem cells, such as HNSC and i-HNSC. | Size: 10 ml | CAT.#: 035-10 | Price: $58.00 | |
| HNSC Differentiation Medium, Astrocyte Lineage: Differentiates HNSC to Astrocytes. Differentiation also requires NSC Differentiation Coating Solutions A & B. | Size: 100 ml | CAT.#: 813D-100A | Price: $73.00 | |
| HNSC Differentiation Medium, Neuron Lineage: Differentiates HNSC to Neurons. Differentiation also requires NSC Differentiation Coating Solutions A & B. | Size: 100 ml | CAT.#: 813D-100N | Price: $88.00 | |
| HNSC Differentiation Medium, Oligodendrocyte Lineage: Differentiates HNSC to Oligodendrocytes. Differentiation also requires NSC Differentiation Coating Solutions A & B. | Size: 100 ml | CAT.#: 813D-100O | Price: $73.00 | |
| HNSC Differentiation Medium: General HNSC Differentiation Medium. Differentiation also requires NSC Differentiation Coating Solutions A & B. | Size: 250 ml | CAT.#: 813D-250 | Price: $152.00 |