MSDS 310-500
MSDS 311-500
MSDS 311A-500
MSDS 311B-500
MSDS 311D-500
MSDS 311F-500
MSDS 311-GS
MSDS 311K-500

Description

 
Human Smooth Muscle Cell Media. Formulated with growth factors, supplements and components to promote optimal culture and growth of SMC isolated from aorta, artery and vein.

Details

CAI media are tested for sterility in order to confirm no bacteria, yeast or fungi contaminate the solutions.  The products undergo further quality control for correct pH, osmolality and lack of endotoxins. A panel of different bioassays affirm the media sustain a proper environment for expected cell-type-specific culture, growth, plating, karyotype, physiology, morphology, viability, population doublings, surface markers, cryopreservation, differentiation and/or induction.
 
Laboratory research use only (RUO). Not for human, clinical, diagnostic or veterinary use.

Products

Product Size CAT.# Price Quantity
Human Smooth Muscle Cell Basal Medium: Basal medium (contains no growth supplement).  Add GS before use. Size: 500 ml CAT.#: 310-500 Price: $57.04
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Human Smooth Muscle Cell Differentiation Medium: Promotes cells to change from one type to another, more specialized Size: 250 ml CAT.#: 311D-250 Price: $73.19
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Human Smooth Muscle Cell Growth Medium: Growth medium without antibiotics Size: 500 ml CAT.#: 311A-500 Price: $113.01
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Human Smooth Muscle Cell Growth Medium: Growth medium without bFGF Size: 500 ml CAT.#: 311b-500 Price: $113.01
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Human Smooth Muscle Cell Growth Medium: Growth medium without FBS Size: 500 ml CAT.#: 311F-500 Price: $113.01
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Human Smooth Muscle Cell Growth Medium: All-in-one ready-to-use Size: 500 ml CAT.#: 311-500 Price: $105.47
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Human Smooth Muscle Cell Growth Medium Kit: Basal medium & growth supplement sold together packaged separately Size: Yields 500ml CAT.#: 311K-500 Price: $113.01
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Human Smooth Muscle Cell Growth Supplement: Added to Basal Medium to create Growth Medium Size: 30 ml CAT.#: 311-GS Price: $57.04
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Related Products

Product Size CAT.# Price Quantity
Cell Freezing Medium: For general cryopreservation of most primary cells. Contains FBS & DMSO. Size: 50 ml CAT.#: 040-50 Price: $53.81
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HCASMC Conditioned Medium: HCASMC Conditioned Medium Size: 5 ml CAT.#: 350cm-005 Price: $48.43
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Human Smooth Muscle Cell Differentiation Medium: Promotes cells to change from one type to another, more specialized Size: 250 ml CAT.#: 311D-250 Price: $73.19
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Subculture Reagent Kit: 100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution Size: 1 Kit CAT.#: 090K Price: $54.89
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Extended Family Products

Product Size CAT.# Price Quantity
Cyto-X Colorimetric Cell Counting Reagent: 500 tests Size: 1 Bottle CAT.#: 028-01 Price: $138.84
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Cyto-X Colorimetric Cell Counting Reagent: 100 tests Size: Sample CAT.#: 028-S Price: $35.88
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Cytofect™ Smooth Muscle Cell Transfection Kit: 25 x 24-Well Rxns Size: 1 Sample Kit CAT.#: TF350KS Price: $53.76
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Cytofect™ Smooth Muscle Cell Transfection Kit: 125 x 24-Well Size: 1 Kit CAT.#: TF350K Price: $285.21
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Resources/Documents

Publications

2017

Kikuchi, S., Chen, L., Xiong, K., Saito, Y., Azuma, N., Tang, G., Sobel, M., Wight, T.N. and Kenagy, R.D., 2017. Smooth muscle cells of human veins show an increased response to injury at valve sites. Journal of Vascular Surgery. DOI: 10.1016/j.jvs.2017.03.447.

Vanags, L.Z., Tan, J.T., Santos, M., Michael, P.S., Ali, Z., Bilek, M.M., Wise, S.G. and Bursill, C.A., 2017. Plasma activated coating immobilizes apolipoprotein AI to stainless steel surfaces in its bioactive form and enhances biocompatibility. Nanomedicine: Nanotechnology, Biology and Medicine. https://doi.org/10.1016/j.nano.2017.06.012.

2016
Cooper, K., I. Chun, D. Colter, S. Dhanaraj, A. Gosiewska, A. Seyda, C. Fang and C. Yang. 2016. Tissue engineered blood vessel. Patent US 9290742 B2.
Franzoni, M., I. Cattaneo, L. Longaretti, M. Figliuzzi, B. Ene-Iordache and A. Remuzzi. 2016. Endothelial cell activation by hemodynamic shear stress derived from arteriovenous fistula for hemodialysis accessAm J Physiol Heart Circ Physiol 310:H49 –H59.
Gu, H., X. Chen, Q. Yu, X. Liu, W. Zhan, H. Chen and J. Brash. 2016. A multifunctional surface for blood contact with fibrinolytic activity, ability to promote endothelial cell adhesion and inhibit smooth muscle cell adhesion. J Mater Chem B, DOI: 10.1039/c6tb02808.
Kikuchi, S., R. Kenagy, L. Gao, T. Wight, N. Azuma, M. Sobel and A. Clowes. 2016. Surgical marking pen dye inhibits saphenous vein cell proliferation and migration in saphenous vein graft tissue. J Vasc Surg, 65:1044-1050.
Lamichhane, S., J. Anderson, T. Remund, H. Sun, M. Larson, P. Kelly and G. Mani. 2016. Responses of endothelial cells, smooth muscle cells, and platelets dependent on the surface topography of polytetrafluoroethylene. J Biomed Mat Res, 104:2291-2304.
Maier, K., B. Ruhle, J. Stein, K. Gentile, F. Middleton and V. Gahtan. 2016. Thrombospondin-1 differentially regulates microRNAs in vascular smooth muscle cellsMolec & Cell Biochem, 412:111-117.
Mani, G., S. Lamichhane, J. Anderson, T. Remund and P. Kelly. 2016. Formulations for Tailored Drug ReleasePatent Application US 20160121027 A1.
Scott, T., O. Babayeva, S. Banerjee, W. Zhong, and S. Francis. 2016. SGK1 is modulated by resistin in vascular smooth muscle cells and in the aorta following diet-induced obesity. Obesity, 24:678-686.
2015
Anderson, J., T. Remund, K. Pohlson, S. Lamichhane, C. Evans, R. Evans, M. Clark, K. Egland, P. Kelly and G. Mani. 2015. In vitro and in vivo evaluation of effect of excipients in local delivery of paclitaxel using microporous infusion balloon catheters. J Biomed Mater Res Part B, DOI: 10.1002/jbm.b.33564.
Baskar, K., S. Sur, V. Selvaraj and D. Agrawal. 2015. Functional constituents of a local serotonergic system, intrinsic to the human coronary artery smooth muscle cells. Molec Biol Repts, 42:1295-1307.
Lamichhane, S., J. Anderson, T. Remund, P. Kelly and G. Mani. 2015. Dextran sulfate as a drug delivery platform for drug-coated balloons: Preparation, characterization, in vitro drug elution, and smooth muscle cell response. J Biomed Mater Res Part B, 104:1416-1430.
Rao, J., B. Brown, J. Weinbaum, E. Ofstun, M. Makaroun, J. Humphrey, and D. Vorp. 2015. Distinct macrophage phenotype and collagen organization within the intraluminal thrombus of abdominal aortic aneurysm. Journal of Vascular Surgery, doi:10.1016/j.jvs.2014.11.086.
2014
Phillippi, J., B. Green, M. Eskay, M. Kotlarczyk, M. Hill, A. Robertson, S. Watkins, D. Vorp and T. Gleason. 2014. Mechanism of aortic medial matrix remodeling is distinct in patients with bicuspid aortic valve. J Thoracic & Cardiovasc Surg, 147:1056-1064.
Thakali, K., J. Saben, J. Faske, F. Lindsey, H. Gomez-Acevedo, C. Lowery, T. Badger A. Andres and K. Shankar. 2014. Maternal pregravid obesity changes gene expression profiles toward greater inflammation and reduced insulin sensitivity in umbilical cord. Pediatric Res, 76:202-210.
2012
Albasanz-Puig, A., J. Murray, M. Namekata, and E.S. Wijelath. 2012. Opposing roles of STAT-1 and STAT-3 in regulating vascular endothelial growth factor expression in vascular smooth muscle cells. Biochem. & Biophys. Res. Comm. 428:179-184.
Csiszar, A., D. Sosnowska, M. Wang, E. Lakatta, W. Sonntag, and Z. Ungvari. 2012. Age-Associated Proinflammatory Secretory Phenotype in Vascular Smooth Muscle Cells From the Non-human Primate Macaca mulatta: Reversal by Resveratrol Treatment. J Gerontol A Biol Sci Med Sci, 67:811-820.
Jemy, J. 2012. Does Human Leukocyte Antigen-G (HLA-G) Play a Role in Immunte Modulation and Vasculopathy in Heart Transplantation?  Masters Thesis, U Toronto.
Ko, W.-c., B.-c. Chen, M.-j. Hsu, C.-t. Tsai, C.-y. Hong, and C.-h. Lin. 2012. Thrombin induced connective tissue growth factor expression in rat vascular smooth muscle cells via the PAR-1/JNK/AP-1 pathway. Acta pharmacologica Sinica. 33:49-56.
Linde, C., L. Antos, V. Golovina and M. Blaustein. 2012. Nanomolar ouabain increases NCX1 expression and enhances Ca2+ signaling in human arterial myocytes: a mechanism that links salt to increased vascular resistance? Am J Physiol - Heart & Circ Physiol. 303:H784-H794.
Nagao, H., T. Setoguchi, S. Kitamoto, Y. Ishidou, S. Nagano, M. Yokouchi, M. Abematsu, N. Kawabata, S. Maeda, S. Yonezawa, and S. Komiya. 2012. RBPJ Is a Novel Target for Rhabdomyosarcoma Therapy. PloS one. 7:e39268. 
2011
Kanie, K., R. Kato, Y. Zhao, Y. Narita, M. Okochi, and H. Honda. 2011. Amino acid sequence preferences to control cell‐specific organization of endothelial cells, smooth muscle cells, and fibroblasts. J Peptide Res, 17:479-486.
Lee, Y.-L., C.-H. Chiao, and M.-T. Hsu. 2011. Transcription of Muscle Actin Genes by a Nuclear Form of Mitochondrial RNA Polymerase. PloS one. 6:e22583.
Ungvari, Z., L. Bailey-Downs, T. Gautam, D. Sosnowska, M. Wang, R. Monticone, R. Telljohann, J. Pinto., R. Cabo, W. Sonntag, E. Lakatta, and A. Csiszar. 2011. Age-associated vascular oxidative stress, Nrf2 dysfunction, and NF-κB activation in the nonhuman primate Macaca mulatta. J Gerontol A Biol Sci Med Sci, 66:866-875.
2010
Brito, L.A., S. Chandrasekhar, S.R. Little, and M.M. Amiji. 2010. In vitro and In vivo studies of local arterial gene delivery and transfection using lipopolyplexes‐embedded stents. Journal of Biomedical Materials Research Part A. 93:325-336.
Maier, K., X. Han, B. Sadowitz, K. Gentile, F. Middleton and V. Gahtan. 2010. Thrombospondin-1: A proatherosclerotic protein augmented by hyperglycemia. J Vasc Surg, 51:1238-1247.
Nivison-Smith, L., J. Rnjak, and A.S. Weiss. 2010. Synthetic human elastin microfibers: Stable cross-linked tropoelastin and cell interactive constructs for tissue engineering applications. Acta Biomaterialia. 6:354-359.