Description
Human iPSC-Derived Neural Stem Cells (i-HNSC) are a homogeneous, self-renewing, multipotent population generated from control human iPSCs. Under defined conditions, they yield high numbers of functional neurons and glia for studies of development, neurotoxicity, differentiation, electrophysiology, and disease.
Cultured on Cell Applications’s Neural Longevity Matrix (NLM, Cat# 1041K-2), i-HNSC sustain adherent proliferation over multiple doublings without loss of stemness. For differentiation, seed on NSC Differentiation Coating Solutions A & B (Cat# 034-10, 035-10) and use lineage-specific media—Neuron (Cat# 813D-100), Astrocyte (Cat# 813D-100A), Oligodendrocyte (Cat# 813D-100O)—within defined differentiation media. Lineage identity is confirmed by β-tubulin III (neurons), GFAP (astrocytes), and O4 (oligodendrocytes).
This platform delivers a physiologically relevant, scalable system for CNS modeling and translational research. i-HNSC-derived neurons and astrocytes, especially in co-culture, enhance neuronal maturation and long-term survival.¹²³ In defined media, i-HNSC naturally generate a balanced neuron–astrocyte population, improving the predictive power of drug screening. Next-generation manufacturing enables rapid, large-lot production from a single donor.
- Tang X, Zhou L, Wagner AM, Marchetto MCN, Muotri AR, et al. Astroglial cells regulate the developmental timeline of human neurons differentiated from induced pluripotent stem cells. 2013. Stem Cell Res 11, 743–757.
- Odawara, A., Saitoh, Y., Alhebshi, A. H., Gotoh, M. & Suzuki, I. Long-term electrophysiological activity and pharmacological response of a human induced pluripotent stem cell-derived neuron and astrocyte co-culture. 2014. Biochem Biophys Res Commun 443, 1176–1181.
- Fukushima, K., Miura, Y., Sawada, K., Yamazaki, K. & Ito, M. Establishment of a Human Neuronal Network Assessment System by Using a Human Neuron/Astrocyte Co-Culture Derived from Fetal Neural Stem/Progenitor Cells 2016. J Biomol Screen 21, 54–64.
- Marchetto, M. C. N., Carromeu, C., Acab, A., Yu, D., Yeo, G., Yangling, M., Gage, F. H. & Muotri, A. R. A model for neural development and treatment of Rett syndrome using human induced pluripotent stem cells. Cell, 143(4): 527-39 (2010).
Details
| Source | Derived from Human iPSC of a single donor |
| QC | No bacteria, yeast, fungi, mycoplasma, virus |
| CofA | Donor, gender, tissue source, disease status, cell viability (post-thaw), passage #, karyotype, immunostaining markers |
| Cryovial | 1,000,000 i-HNSC (1st passage) in freezing medium |
| Medium | Cells manufactured, maintained in serum-free, feeder-free culture conditions |
| Storage | Liquid Nitrogen |
| Bioassay | NSC morphology, growth behavior when grown in supportive conditions |
| Applications | Laboratory research use only (RUO). Not for human, clinical, diagnostic or veterinary use. |
Products
| Product | Size | CAT.# | Price | Quantity |
|---|---|---|---|---|
| Cryopreserved Induced Human Neural Stem CellsTotal Kit: 1x10^6 Cells, Coating Solution, Medium and Dissociation Solution | Size: 1 Kit | CAT.#: i820K-10 | Price: $977.00 | |
| Cryopreserved iPSC-derived Neural Stem Cells (i-HNSC): Frozen Human iPSC-Derived Neural Stem Cells (1x10^6) (See Details for age, gender) | Size: 1 Cryovial | CAT.#: i820-10 | Price: $850.00 |
Related Products
| Product | Size | CAT.# | Price | Quantity |
|---|---|---|---|---|
| i-HNSC Growth Medium Kit : Basal medium & growth supplement sold together packaged separately. | Size: 250 ml | CAT.#: i813K-250 | Price: $198.00 | |
| i-HNSC Growth Medium Kit: Basal medium & growth supplement sold together packaged separately. | Size: 100 ml | CAT.#: i813K-100 | Price: $106.00 | |
| Neural Longevity Matrix (NLM): Specially formulated proprietary coating colution kit for 2D culture of neural cells. For differentiation of NSC to neural lineages, use NSC Differentiation Coating Solutions A & B. | Size: Yields 2 ml | CAT.#: 1041K-2 | Price: $125.00 | |
| Neural Stem Cell Differentiation Coating Solution A: Coating Solution A used specifically for the differentiation of neural stem cells, such as HNSC and i-HNSC. | Size: 10 ml | CAT.#: 034-10 | Price: $58.00 | |
| Neural Stem Cell Differentiation Coating Solution B: Coating Solution B used specifically for the differentiation of neural stem cells, such as HNSC and i-HNSC. | Size: 10 ml | CAT.#: 035-10 | Price: $58.00 | |
| HNSC Differentiation Medium, Astrocyte Lineage: Differentiates HNSC to Astrocytes. Differentiation also requires NSC Differentiation Coating Solutions A & B. | Size: 100 ml | CAT.#: 813D-100A | Price: $73.00 | |
| HNSC Differentiation Medium, Neuron Lineage: Differentiates HNSC to Neurons. Differentiation also requires NSC Differentiation Coating Solutions A & B. | Size: 100 ml | CAT.#: 813D-100N | Price: $88.00 | |
| HNSC Differentiation Medium, Oligodendrocyte Lineage: Differentiates HNSC to Oligodendrocytes. Differentiation also requires NSC Differentiation Coating Solutions A & B. | Size: 100 ml | CAT.#: 813D-100O | Price: $73.00 | |
| HNSC Differentiation Medium: General HNSC Differentiation Medium. Differentiation also requires NSC Differentiation Coating Solutions A & B. | Size: 250 ml | CAT.#: 813D-250 | Price: $152.00 | |
| i-HNSC Dissociation Solution : Dissociates neural stem cells. | Size: 25 ml | CAT.#: 076-50 | Price: $48.00 | |
| Freezing Medium for Neurons wo Serum: Serum-free freezing medium specialized for neurons | Size: 50 ml | CAT.#: 042-50 | Price: $98.00 |
Extended Family Products
| Product | Size | CAT.# | Price | Quantity |
|---|---|---|---|---|
| Cytofect Neuron Transfection Kit (100 x 24-Wells): 100 x 24-Well Rxns | Size: 1 Kit | CAT.#: TF886K | Price: $512.00 | |
| Cytofect Neuron Transfection Sample Kit (25 x 24-Wells): 25 x 24-Well Rxns | Size: 1 Sample Kit | CAT.#: TF886KS | Price: $68.00 |
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