Description
Alveolar type 2 cells (AEC2) function in ion transport and epithalium repair, as well as in the defense and immune response against microorganisms. AEC2 secrete surfactant proteins, release a host of factors that include cytokines and chemokines, and present antigen to specific T cells.
Cell Applications' AEC2 / i-HAEpC2 are Type 2 Alveolar Epithelial Cells that were derived from induced pluripotent stem cells with our unique technology. iPSC undergo multistage differentiation to AEC2, and are characterized by staining for NK2 homeobox 1 (NKX2-1), a marker for lung differentiation lineage, and Surfactant protein C (SFTPC), a marker for Alveolar Type 2 cells. The i-Alveolar Epithelial Cell Total Kit includes cryopreserved AEC2 / i-HAEpC2, optimized growth medium, coating medium, and dissociation solution.
AEC2 / i-HAEpC2. Top: Phase contrast, 100x. Bottom left: Immunostaining for ACE2 (green) and SFTPC (red). Bottom right: Immunostaining for SFTPB (green) and NKX2.1 (red).
Details
| Source | Derived from Human iPSC of a single donor |
| QC | No bacteria, yeast, fungi, mycoplasma, virus |
| Bioassay | Attach, spread, proliferate in Growth Med |
| Cryovial | 500,000 AEC2 / i-HAEpC2 (1st passage) in freezing medium |
| Kit | Cryovial frozen AEC2 / i-HAEpC2 (i536-05), Gr Med Kit (519K-100), Coating Med (1020-10), Dissociation Kit (077-10) |
| Cultured | Shipped in Tsfr Med (flasks or plates) |
| Doublings | At least 8 |
| Applications | Laboratory research use only (RUO). Not for human, clinical, diagnostic or veterinary use. |
Products
| Product | Size | CAT.# | Price | Quantity |
|---|---|---|---|---|
| Cryopreserved AEC2 / i-HAEpC2: 5x10^5 Cells, Growth Media Kit, Coating Media, and Dissociation Kit (See Details tab for specifics) | Size: 1 Kit | CAT.#: i536K-05 | Price: $1,663.00 | |
| Cryopreserved AEC2 / i-HAEpC2: Frozen i-HAEpC2 (5x10^5) | Size: 1 Cryovial | CAT.#: i536-05 | Price: $1,475.00 | |
| Proliferating AEC2 / i-HAEpC2: Cell culture in medium | Size: T-75 Flask | CAT.#: i537-75 | Price: $1,995.00 | |
| Proliferating AEC2 / i-HAEpC2: Cell culture in medium | Size: 96 Well | CAT.#: i537-96w | Price: $1,575.00 |
Related Products
| Product | Size | CAT.# | Price | Quantity |
|---|---|---|---|---|
| AEC2 / i-HAEpC2 Growth Medium Kit: Basal medium & growth supplement sold together packaged separately. Keep GS frozen for up to 6 months. Once thawed, use within 4 weeks. | Size: Yields 100ml | CAT.#: 519K-100 | Price: $158.00 | |
| AEC2 / i-HAEpC2 Basal Medium: Without growth supplement (GS). Add GS before use. | Size: 100 ml | CAT.#: 518-100 | Price: $35.00 | |
| AEC2 / i-HAEpC2 Growth Supplement: Added to Basal Medium to create Growth Medium. | Size: 3 ml | CAT.#: 519-GS | Price: $123.00 | |
| AEC2 / i-HAEpC2 Coating Medium: Coating Solution for i-HAEpC | Size: 10 ml | CAT.#: 1020-10 | Price: $25.00 | |
| AEC2 / i-HAEpC2 Dissociation Solution: Dissociation solution for AEC2 / i-HAEpC2 | Size: 10 ml | CAT.#: 077-10 | Price: $25.00 |