MSDS Cryopreserved Cells
Instructions AEC2 i-HAEpC2
Cell Apps Flyer Epithelial Cells
5 Important Cell Culture Rules
Cell Apps Poster Primary Cells
Cell Applications Inc Brochure

Description

Alveolar type 2 cells (AEC2) function in ion transport and epithalium repair, as well as in the defense and immune response against microorganisms. AEC2 secrete surfactant proteins, release a host of factors that include cytokines and chemokines, and present antigen to specific T cells.

Cell Applications' AEC2 / i-HAEpC2 are Type 2 Alveolar Epithelial Cells that were derived from induced pluripotent stem cells with our unique technology. iPSC undergo multistage differentiation to AEC2, and are characterized by staining for NK2 homeobox 1 (NKX2-1), a marker for lung differentiation lineage, and Surfactant protein C (SFTPC), a marker for Alveolar Type 2 cells. The i-Alveolar Epithelial Cell Total Kit includes cryopreserved AEC2 / i-HAEpC2, optimized growth medium, coating medium, and dissociation solution.

 

AEC2 / i-HAEpC2. Top: Phase contrast, 100x. Bottom left: Immunostaining for ACE2 (green) and SFTPC (red). Bottom right: Immunostaining for SFTPB (green) and NKX2.1 (red).

Details

SourceDerived from Human iPSC of a single donor
QCNo bacteria, yeast, fungi, mycoplasma, virus
BioassayAttach, spread, proliferate in Growth Med
Cryovial500,000 AEC2 / i-HAEpC2 (1st passage) in freezing medium
KitCryovial frozen AEC2 / i-HAEpC2 (i536-05), Gr Med Kit (519K-100), Coating Med (1020-10), Dissociation Kit (077-10)
CulturedShipped in Tsfr Med (flasks or plates)
DoublingsAt least 8
ApplicationsLaboratory research use only (RUO). Not for human, clinical, diagnostic or veterinary use.
Instructions AEC2 i-HAEpC2

Format: PDF

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Products

Product Size CAT.# Price Quantity
Cryopreserved AEC2 / i-HAEpC2:  5x10^5 Cells, Growth Media Kit, Coating Media, and Dissociation Kit (See Details tab for specifics)  Size: 1 Kit CAT.#: i536K-05 Price: $1,663.00
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Cryopreserved AEC2 / i-HAEpC2: Frozen i-HAEpC2 (5x10^5) Size: 1 Cryovial CAT.#: i536-05 Price: $1,475.00
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Proliferating AEC2 / i-HAEpC2: Cell culture in medium Size: T-75 Flask CAT.#: i537-75 Price: $1,995.00
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Proliferating AEC2 / i-HAEpC2: Cell culture in medium Size: 96 Well CAT.#: i537-96w Price: $1,575.00
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Related Products

Product Size CAT.# Price Quantity
AEC2 / i-HAEpC2 Growth Medium Kit: Basal medium & growth supplement sold together packaged separately. Keep GS frozen for up to 6 months. Once thawed, use within 4 weeks. Size: Yields 100ml CAT.#: 519K-100 Price: $158.00
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AEC2 / i-HAEpC2 Basal Medium: Without growth supplement (GS).  Add GS before use. Size: 100 ml CAT.#: 518-100 Price: $35.00
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AEC2 / i-HAEpC2 Growth Supplement: Added to Basal Medium to create Growth Medium. Size: 3 ml CAT.#: 519-GS Price: $123.00
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AEC2 / i-HAEpC2 Coating Medium: Coating Solution for i-HAEpC Size: 10 ml CAT.#: 1020-10 Price: $25.00
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AEC2 / i-HAEpC2 Dissociation Solution: Dissociation solution for AEC2 / i-HAEpC2 Size: 10 ml CAT.#: 077-10 Price: $25.00
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Resources/Documents

Cell Apps Flyer Epithelial Cells

Format: PDF

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5 Important Cell Culture Rules

Format: PDF

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Cell Apps Poster Primary Cells

Format: PDF

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Cell Applications Inc Brochure

Format: PDF

Downoad Now