Instructions HFLS Normal
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Human Fibroblast-Like Synoviocytes (HFLS) provide an excellent cellular model for studying the normal and pathological physiology of synoviocytes and development of joint diseases.
Normal HFLS from Cell Applications, Inc. have been used to examine:
  • TNFα – mediated changes in gene expression
  • TNF-like weak inducer of apoptosis (TWEAK) contribution to joint inflammation by inducing chemokines and matrix metalloprotease-9,  suggesting TWEAK as a new therapeutic target
  • The role of miR-124a in arthritis pathogenesis
  • C/EBPβ regulation of metalloproteinases and ADAMTS family members in synoviocytes stimulated with IL-1β
  • HMW-HA suppression of ADAMTS4 mRNA and protein expression via CD44, p38 MAPK and JNK pathways
  • Anti-inflammatory and antirheumatic activity of various compounds, such as NF-κB inhibitors, AGIX-4207, HA–methotrexate conjugates and bucillamine
  • Reactive arthritis triggered by chlamydial infection
  • Mechanisms of arthritis-like syndrome in patients infected with chikungunya (CHIK) virus
  • Use of Leukocyte-poor, RBC-free platelet-rich plasma in clinical orthopaedics, because leukocytes and RBCs cause synoviocyte death and proinflammatory mediator production
  • Cartilage link protein and MAGP2 as markers to distinguish chondrocytes and synovial cells
  • Creation of induced pluripotent stem cells (iPSC) by using the now classic “Yamanaka cocktail”, the discovery for which Dr. Shinya Yamanaka was awarded the Nobel Prize in 2012
  • Causative agents, immunopathological mechanisms and signal transduction pathways leading to joint inflammation in rheumatoid arthritis
  • The role of estrogen signaling in increasing inflammation
  • Anti-inflammatory properties of herbal compound Sinomenine suggested for rheumatoid arthritis treatment
  • Effects of extracellular matrix composition on cell attachment and migration relevant to T-cell function in inflamed tissues
  • The role of human endogenous retroviruses (HERVs) in development of rheumatoid arthritis
  • Activated expression of different forms of HERV, contributing to development of rheumatoid arthritis symptoms by different mechanisms
(Click to Enlarge) Human Fibroblast-Like Synoviocytes: HFLS, produce synovial fluid to lubricate joints.


Normal healthy human synovial tissue
No bacteria, yeast, fungi, mycoplasma, virus
Attach, spread, proliferate in Growth Med
500,000 HFLS (2nd passage) frozen in Basal Medium w/ 10% FBS, 10% DMSO
Cryovial frozen HFLS (408-05a), Growth Medium (415-500), Subcltr Rgnt Kit (090K)
Shipped in Gr Med, 3rd psg (flasks or plates)
At least 5
Laboratory research use only (RUO). Not for human, clinical, diagnostic or veterinary use.
Instructions HFLS Normal

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MSDS Cryopreserved Cells

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Product Size CAT.# Price Quantity
Cryopreserved Human Fibroblast-Like Synoviocyte Normal Tissue Total Kit, adult: 5x10^5 Cells (Adult), Medium & Subculture Reagents (See Details tab for specifics). Size: 1 Kit CAT.#: 408K-05a Price: $1,065.00
Cryopreserved Synoviocytes (HFLS), adult: Frozen HFLS (5x10^5). Size: 1 Cryovial CAT.#: 408-05a Price: $895.00
Proliferating Synoviocytes (HFLS), adult: Actively growing, dividing cells in medium. Estimated release June 2021. Size: T-25 Flask CAT.#: 409-25a Price: $895.00
Proliferating Synoviocytes (HFLS), adult: Actively growing, dividing cells in medium. Estimated release June 2021. Size: T-75 Flask CAT.#: 409-75a Price: $1,085.00
Proliferating Synoviocytes (HFLS), adult: Actively growing, dividing cells in medium. Estimated release June 2021. Size: 96 Well CAT.#: 409-96Wa Price: $1,205.00

Related Products

Product Size CAT.# Price Quantity
HFLS Growth Medium: All-in-one ready-to-use Size: 500 ml CAT.#: 415-500 Price: $117.00
HFLS Growth Medium Kit: Basal medium & growth supplement sold together packaged separately Size: Yields 500 ml CAT.#: 415K-500 Price: $128.00
HFLS Basal Medium: Basal medium (contains no growth supplement).  Add GS before use. Size: 500 ml CAT.#: 414-500 Price: $70.00
HFLS Growth Supplement: Added to Basal Medium to create Growth Medium Size: 50 ml CAT.#: 415-GS Price: $74.00
HFLS Growth Medium wo Antibiotics: Growth medium without antibiotics Size: 500 ml CAT.#: 415A-500 Price: $128.00
HFLS Growth Medium wo FBS: Growth medium without FBS Size: 500 ml CAT.#: 415F-500 Price: $128.00

Extended Family Products

Product Size CAT.# Price Quantity
Synoviocytes RNA (HFLS RNA), Adult: Total RNA prepared from Human Fibroblast-Like Synoviocytes, adult Size: 10 ug CAT.#: 408-R10a Price: $398.00
Synoviocytes RNA (HFLS RNA), Adult: Total RNA prepared from Human Fibroblast-Like Synoviocytes, adult Size: 25 ug CAT.#: 408-R25a Price: $796.00


Cell Apps Flyer Skeletal System Cells

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5 Important Cell Culture Rules

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Cell Apps Poster Primary Cells

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Cell Applications Inc Brochure

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