Instructions HA
5 Important Cell Culture Rules
MSDS Cryopreserved Cells
Cell Apps Flyer Nervous System
Cell Apps Flyer Brain Cells
Cell Apps Poster Primary Cells
Cell Applications Inc Brochure

Description

Human astrocytes (HA) are derived from human cerebral cortex.  Astrocytes are the most abundant cells in the central nervous system where they perform many functions, such as providing mechanical support and nutrients to neurons and removal of wastes from neurons; providing signaling to endothelial cells; regulating neurogenesis and controlling synaptic function.  As the recognition of the importance of astrocytes in nervous system is increasing, HA serve as a useful in vitro model for exploring the diversity of astrocytes functions.
Human Astrocytes from Cell Applications, Inc. have been used to:
  • Investigate aberrant gene expression. 
  • Reveal that CBX6 overexpression inhibits proliferation of glyoblastoma cells and
  • Demonstrate the role of epigenetics in glyioblastoma malignancy.
  • Investigate choline uptake system in normal and glioblastoma cells with the hope to improve tomography imaging in glioblastoma patients
Characterization:  Stained positive for GFAP
(Click to Enlarge) Human Astrocytes (HA) (L)HA immunolabeled with anti-GFAP antibodies (green); nuclei are counterstained with PI (red) (R).

Details

Tissue
Normal healthy Human brain
QC
No bacteria, yeast, fungi, mycoplasma, virus
Character
Positive for GFAP
Bioassay
Attach, spread, proliferate in Growth Medium
Cryovial
500,000 HA (2nd passage) frozen in Basal Medium w/10% FBS, 10% DMSO
Kit
Cryovial frozen HA (882A-05), Grwoth Medium (821-500), Subculture Rgnt Kit (090K)
Proliferating
Shipped in Gr Med, 3rd psg (flasks or plates)
Doublings
At least 10
Applications
Laboratory research use only (RUO). Not for human, clinical, diagnostic or veterinary use.
Instructions HA

Format: PDF

Download Now
MSDS Cryopreserved Cells

Format: PDF

Download Now

Products

Product Size CAT.# Price Quantity
Cryopreserved HA, Fetal: Frozen HA (5x10^5) Size: 1 Cryovial CAT.#: 882A-05f Price: $679.00
-+
Cryopreserved HA, Fetal: 5x10^5 Cells (Fetal), Medium & Subculture Reagents (See Details tab for specifics) Size: 1 Kit CAT.#: 882AK-05f Price: $833.00
-+
Proliferating HA, Fetal: Actively growing, dividing cells, in medium Size: T-25 Flask CAT.#: 883A-25f Price: $679.00
-+
Proliferating HA, Fetal: Actively growing, dividing cells, in medium Size: 24 Well CAT.#: 883A-24Wf Price: $869.00
-+
Proliferating HA, Fetal: Actively growing, dividing cells, in medium Size: T-75 Flask CAT.#: 883A-75f Price: $869.00
-+
Proliferating HA, Fetal: Actively growing, dividing cells, in medium Size: 96 Well CAT.#: 883A-96Wf Price: $989.00
-+

Related Products

Product Size CAT.# Price Quantity
HA Basal Medium: Basal medium (contains no growth supplement).  Add GS before use. Size: 500 ml CAT.#: 820-500 Price: $67.00
-+
HA Growth Medium: All-in-one ready-to-use Size: 500 ml CAT.#: 821-500 Price: $109.00
-+
HA Growth Medium Kit: Basal medium & growth supplement sold together packaged separately Size: Yields 500 ml CAT.#: 821K-500 Price: $118.00
-+
HA Growth Supplement: Added to Basal Medium to create Growth Medium Size: 15 ml CAT.#: 821-GS Price: $51.00
-+

Extended Family Products

Product Size CAT.# Price Quantity
Cyto-X Cell Counting Reagent: 500 tests Size: 1 Bottle CAT.#: 028-01 Price: $139.00
-+
Cyto-X Cell Counting Reagent Sample: 100 tests Size: Sample CAT.#: 028-S Price: $36.00
-+
Freezing Medium: For general cryopreservation of most primary cells. Contains FBS & DMSO. Size: 50 ml CAT.#: 040-50 Price: $54.00
-+
HA RNA, Fetal: Total RNA prepared from Human Astrocytes, fetal Size: 10 ug CAT.#: 882A-R10f Price: $409.00
-+
HA RNA, Fetal: Total RNA prepared from Human Astrocytes, fetal Size: 25 ug CAT.#: 882A-R25f Price: $818.00
-+
Human Brain RNA, Fetal: Total RNA prepared from human brain tissue, fetal Size: 50 ug CAT.#: 1F01-50 Price: $139.00
-+
Human Brain RNA, Fetal: Total RNA prepared from human brain tissue, fetal Size: 250 ug CAT.#: 1F01-250 Price: $522.00
-+
Subculture Reagent Kit: 100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution Size: 1 Kit CAT.#: 090K Price: $55.00
-+

Resources/Documents

5 Important Cell Culture Rules

Format: PDF

Downoad Now
Cell Apps Flyer Nervous System

Format: PDF

Downoad Now
Cell Apps Flyer Brain Cells

Format: PDF

Downoad Now
Cell Apps Poster Primary Cells

Format: PDF

Downoad Now
Cell Applications Inc Brochure

Format: PDF

Downoad Now

Publications

2012
Wang, P., F. Zhu and K. Konstantopoulos. 2012. The Antagonistic Actions of Endogenous Interleukin-1β and 15-Deoxy-Δ12,14-prostaglandin J2 Regulate the Temporal Synthesis of Matrix Metalloproteinase-9 in Sheared Chondrocytes.
2014
Taguchi, C., M. Inazu, I. Saiki, M. Yara, N. Hara, T. Yamanaka, and H. Uchino. 2014. Functional analysis of [methyl-3H]choline uptake in glioblastoma cells: influence of anti-cancer and central nervous system drugs. Biochemical pharmacology. 88:303-312.
2013
Li, G., C. Warden, Z. Zou, J. Neman, J.S. Krueger, A. Jain, R. Jandial, and M. Chen. 2013. Altered Expression of Polycomb Group Genes in Glioblastoma Multiforme. PloS one. 8:e80970.