MSDS 810-500
MSDS 811-500
MSDS 811-GS
MSDS 811D-250
MSDS 811DS-250
MSDS 811K-500
MSDS 811M-250
MSDS 811Mi-250
MSDS 811S-250

Description

Medium carefully optimized for the characteristics and requirements unique to Human Adipocytes & Preadipocytes.  Attention to detail ensures ideal cell health, viability, performance, physiology, morphology, consistency and data.
 
Use with:
Human Adipocyte & Preadipocyte Media

Details

CAI media are tested for sterility in order to confirm no bacteria, yeast or fungi contaminate the solutions.  The products undergo further quality control for correct pH, osmolality and lack of endotoxins. A panel of different bioassays affirm the media sustain a proper environment for expected cell-type-specific culture, growth, plating, karyotype, physiology, morphology, viability, population doublings, surface markers, cryopreservation, differentiation and/or induction.
 
Laboratory research use only (RUO). Not for human, clinical, diagnostic or veterinary use.

Products

Product Size CAT.# Price Quantity
HPAd Differentiation Medium: Add to preadipocytes and mesenchymal stem cells to differentiate into adipocytes Size: 250 ml CAT.#: 811D-250 Price: $95.00
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HPAd Growth Medium: All-in-one ready-to-use Size: 500 ml CAT.#: 811-500 Price: $135.00
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HPAd Basal Medium: Basal medium (contains no growth supplement).  Add GS before use. Size: 500 ml CAT.#: 810-500 Price: $77.00
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HPAd Growth Supplement: Added to Basal Medium to create Growth Medium Size: 50 ml CAT.#: 811-GS Price: $59.00
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HPAd Growth Medium Kit: Basal medium & growth supplement sold together packaged separately Size: Yields 500 ml CAT.#: 811K-500 Price: $148.00
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HAd Maintenance Medium: Maintenance medium for adipocytes Size: 250 ml CAT.#: 811M-250 Price: $68.00
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Adipoccyte Starvation Medium: Use when cells need to be starved overnight to 24 hrs before experiment Size: 250 ml CAT.#: 811S-250 Price: $62.00
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HPAd Differentiation Medium, Component Kit: Differentiation medium components sold together packaged separately Size: Yields 250 ml CAT.#: 811DS-250 Price: $132.00
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HAd Maintenance Medium wo Insulin: Maintenance Medium for adipocytes without insulin Size: 250 ml CAT.#: 811Mi-250 Price: $78.00
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Related Products

Product Size CAT.# Price Quantity
Freezing Medium: For general cryopreservation of most primary cells. Contains FBS & DMSO. Size: 50 ml CAT.#: 040-50 Price: $54.00
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Subculture Reagent Kit: 100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution Size: 1 Kit CAT.#: 090K Price: $55.00
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Extended Family Products

Product Size CAT.# Price Quantity
Cyto-X Cell Counting Reagent: 500 tests Size: 1 Bottle CAT.#: 028-01 Price: $139.00
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Cyto-X Cell Counting Reagent Sample: 100 tests Size: Sample CAT.#: 028-S Price: $36.00
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Cytofect™ Preadipocyte Transfection Kit: 175 x 24-Well Rxns Size: 1 Kit CAT.#: TF802K Price: $350.00
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Cytofect™ Preadipocyte Transfection Kit: 25 x 24-Well Rxns Size: 1 Sample Kit CAT.#: TF802KS Price: $54.00
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Resources/Documents

Publications

2016
Hantash, B. 2016. Differentiation of mesenchymal stem cells into fibroblasts. Patent US 9347045 B2.
Subbaramaiah, K., K. Brown, H. Zahid, K. Balmus, R. Weiss, B. Herbert and A. Dannenberg. 2016. Hsp90 and PKM2 Drive the Expression of Aromatase in Li-Fraumeni Syndrome Breast Adipose Stromal Cells. J Biol Chem, doi: 10.1074/jbc.M115.698902.
2015
Floyd, E., J. Staszkiewicz, R. Power, G. Kilroy, H. Kirk-Ballard, C. Barnes, K. Strickler, F. Rim, L. Harkins, R. Gao, J. Kim and K. Eilersten. 2015. Prolonged Proteasome Inhibition Cyclically Upregulates Oct3/4 and Nanog Gene Expression, but Reduces Induced Pluripotent Stem Cell Colony Formation. Cellular Reprogramming, 17:95-105.
Ito, Y., H. Ishiguro, N. Kobayashi, H. Hasumi, M. Watanabe, M. Yao and H. Uemura. 2015. Adipocyte-derived monocyte chemotactic protein-1 (MCP-1) promotes prostate cancer progression through the induction of MMP-2 activity. The Prostate, 75:1009-1019.
Lynch, P. E. Thompson, K. McGinnis, Y. Rovira Gonzalez, J. Surdo, S. Bauer and D. Hursh. 2015. Chromatin Changes at the PPAR-γ2 Promoter During Bone Marrow-Derived Multipotent Stromal Cell Culture Correlate With Loss of Gene Activation Potential. Stem Cells, 33:2169-2181.
2014
Hantash, B. and Y. Wang. 2014. Hybrid hydrogel scaffold compositions and methods of use. Patent US 8911723 B2.
You, J., Y. Lee, K. Kim, S. Kim and K. Chang. 2014. Anti-obesity and hypolipidaemic effects of Nelumbo nucifera seed ethanol extract in human pre-adipocytes and rats fed a high-fat diet. J Science of Food & Ag, 94:568-575.
2011
Medici, D., and B. Olsen. 2011. Conversion of vascular endothelial cells into multipotent stem-like cells. Patent Application US 20130078718 A1.
Naruse, K., Y. Yamasaki, T. Tsunemi, A. Onogi, T. Noguchi, T. Sado, H. Oi, and H. Kobayashi. 2011.  Increase of high molecular weight adiponectin in hypertensive pregnancy was correlated with brain-type natriuretic peptide stimulation on adipocyte. Pregnancy Hypertension: An International Journal of Women's Cardiovascular Health. 1:200-205.
Samuvel, D.J., J. Jin, K.P. Sundararaj, Y. Li, X. Zhang, M.F. Lopes-Virella, and Y. Huang. 2011. TLR4 Activation and IL-6-Mediated Cross Talk between Adipocytes and Mononuclear Cells Synergistically Stimulate MMP-1 Expression. Endocrinology. 152:4662-4671.
2010
Samuvel, D.J., K.P. Sundararaj, Y. Li, M.F. Lopes-Virella, and Y. Huang. 2010. Adipocyte-Mononuclear Cell Interaction, Toll-like Receptor 4 Activation, and High Glucose Synergistically Up-regulate Osteopontin Expression via an Interleukin 6-mediated Mechanism. Journal of Biological Chemistry. 285:3916-3927.
2009
Chen, W., V. Yechoor, B. Chang, M. Li, K. March, and L. Chan. 2009. The Human Lipodystrophy Gene Product BSCL2/Seipin Plays a Key Role in Adipocyte Differentiation. Endocrinology, doi:10.1210/en.2009-0236.