Product Sheet RP1200
Product Sheet RP1200AF
Description
BACKGROUND Fibroblast growth factor 5 (FGF-5) is a secreted heparin-binding growth factor that binds to FGF receptors 1 and 2 (FGFR1 and FGFR2). FGF-5 is expressed in the mesenchyme, skeletal muscles, central nervous system, and hair follicles to promote cell differentiation and proliferation. FGF-5 functions as a regulatory factor during hair elongation and skeletal muscle development.
Recombinant human Fibroblast growth factor 5 is a non-glycosylated protein monomer, containing 252 amino acids and having a molecular mass of 27.7 kDa.
Products are for research use only. They are not intended for human, animal, or diagnostic applications.
Details
Cat. No.:
RP1200 / RP1200AF
Alternate Names:
Heparin-binding growth factor 5, HBGF-5, Smag-82
Source:
E. coli
Physical Appearance:
Sterile Filtered white lyophilized (freeze-dried) powder.
Formulation:
10 mM sodium phosphate and 100 mM sodium chloride, pH 7.5
Stability:
Lyophilized product is very stable at -20°C. Reconstituted material should be aliquoted and frozen at -20°C. It is recommended that a carrier protein (0.1% HSA or BSA) is added for long term storage.
Reconstitution:
Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water at 0.1 mg/mL, which can be further diluted into other aqueous solutions.
Protein Content and Purity determined by:
• UV spectroscopy at 280 nm.
• RP-HPLC calibrated against a known standard.
• Quantitation against a known standard via reducing and non-reducing SDS-PAGE gels.
Endotoxin Level:
Endotoxin level, as measured by LAL analysis, is <0.01ng/ug or <0.1EU/ug.
Biological Activity:
The activity is measured by the dose-dependent NR6R-3T3 Proliferation w 1 ug heparin, with Bioactivity Acceptance Criteria ED50 at 10 ng/mL.
AA Sequence:
MAWAHGEKRL APKGQPGPAA TDRNPIGSSS RQSSSSAMSS SSASSSPAAS LGSQGSGLEQ SSFQWSPSGR RTGSLYCRVG IGFHLQIYPD GKVNGSHEAN MLSVLEIFAV SQGIVGIRGV FSNKFLAMSK KGKLHASAKF TDDCKFRERF QENSYNTYAS AIHRTEKTGR EWYVALNKRG KAKRGCSPRV KPQHISTHFL PRFKQSEQPE LSFTVTVPEK KNPPSPIKSK IPLSAPRKNT NSVKYRLKFR FG
Animal Component-Free
This product is produced with no animal derived raw products. All processing and handling employs animal free equipment and animal free protocols.
Products
| Product | Size | CAT.# | Price | Quantity |
|---|---|---|---|---|
| Human Fibroblast Growth Factor 5 (FGF-5): Human Fibroblast Growth Factor-5 | Size: 10 ug | CAT.#: RP1200-10 | Price: $78.00 | |
| Human Fibroblast Growth Factor 5 (FGF-5): Human Fibroblast Growth Factor-5 | Size: 100 ug | CAT.#: RP1200-100 | Price: $329.00 | |
| Human Fibroblast Growth Factor 5 (FGF-5): Human Fibroblast Growth Factor-5 | Size: 1000 ug | CAT.#: RP1200-1000 | Price: $1,704.00 | |
| Human FGF-5, Animal-Free: Human Fibroblast Growth Factor-5, Animal-Free | Size: 10 ug | CAT.#: RP1200AF-10 | Price: $76.00 | |
| Human FGF-5, Animal-Free: Human Fibroblast Growth Factor-5, Animal-Free | Size: 100 ug | CAT.#: RP1200AF-100 | Price: $397.00 | |
| Human FGF-5, Animal-Free: Human Fibroblast Growth Factor-5, Animal-Free | Size: 1000 ug | CAT.#: RP1200AF-1000 | Price: $1,874.00 |