Product Sheet CC1027


BACKGROUND VEGFR1 (Flt-1) and VEGFR2 (KDR/Flk-1) play an important role in regulating physiological as well as pathological angiogenesis. VEGFR2 has strong tyrosine kinase activity, and transduces the major signals for angiogenesis. However, unlike other representative tyrosine kinase receptors which use the Ras pathway, VEGFR2 mostly uses the Phospholipase-C-gamma-Protein kinase-C pathway to activate MAP-kinase and DNA synthesis. VEGFR2 is a direct signal transducer for pathological angiogenesis including cancer and diabetic retinopathy, thus, VEGFR2 itself and the signaling pathway appear to be critical targets for the suppression of these diseases.1,2  Upon binding to its ligand most commonly including VEGF-A and VEGF-E, VEGFR2 undergoes dimerization and becomes activated. Autophosphorylation of tyrosine 1054 and tyrosine 1059 in its kinase catalytic domain is required for tyrosine kinase activity.3
1. Olsson, A. K. et al: Nat Rev Mol Cell Biol 7:359-71, 2006.
2. Shibuya, M.: J Biochem Mol Biol. 39:469-78, 2006.
3. Paz, K. & Zhu, Z.: Front Biosci. 10:1415-39, 2005. 
Products are for research use only. They are not intended for human, animal, or diagnostic applications.

(Click to Enlarge) HUVEC (Cat# 200-05n) were starved overnight and then stimulated with VEGF-A (100ng/ml for 2 min). The cell lysates were subjected to Western blot analysis using Phospho-VEGFR2 (Tyr1054/1059) specific antibody (Cat# CC1027) (Top), and VEGFR2 Antibody (Cat# CB1020) (Bottom).


15 amino acid residues surrounding and including human VEGFR2 phospho-Tyr1054 and phospho-Tyr1059.
Affinity Purified Rabbit IgG
Species & predicted
species cross-
reactivity ( ):
Human, Mouse
Applications &
Suggested starting
WB                  1:1000
IP                    n/d
IHC (Paraffin)  n/d
ICC                  n/d
FACS               n/d
Predicted Molecular
Weight of protein:
230 kDa
This antibody detects endogenous levels of phosphorylated VEGFR2.
Store at 4° C for frequent use; at -20° C for at least one year.

*Optimal working dilutions must be determined by end user.


Product Size CAT.# Price Quantity
Polyclonal VEGF Receptor 2, Phospho-Tyr1054/Tyr1059 Antibody: Polyclonal VEGF Receptor 2, Phospho-Tyr1054/Tyr1059 Antibody Size: 200 ul CAT.#: CC1027 Price: $333.00



Bailey, E., Y Cui, A. Casey, J. Stoler, X. AI, D. Ma, R. Handin, P. Sliz, SVargas and S. El-Chemaly. Pulmonary Vasculopathy Associated with FIGF Gene Mutation. Am J Pathol, 187:25-32.
Bailey, E., Y. Cui, A. Casey, J. Stoler, X. Ai, D. Ma, R. Handin, P. Sliz, S. Vargas and S. El-Chemaly. 2016. Pulmonary Vasculopathy Associated with FIGF Gene Mutation. Am J Pathol, 187:25-32.
Tammali, R., A.M. Reddy, S. Srivastava, and K. Ramana. 2011. Inhibition of aldose reductase prevents angiogenesis in vitro and in vivo. Angiogenesis. 14:209-221.