Product Sheet CP10069
Description
BACKGROUND DDX4 is a member of DEAD box proteins, which are are putative RNA helicases and characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD). They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly.1 Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. DDX4 is a homolog of VASA proteins in Drosophila and several other species.2 It is specifically expressed in the germ cell lineage in both sexes and functions in germ cell development. DDX4 is a highly sensitive and specific marker for germ cells.3 Multiple transcript variants encoding different isoforms have been found for DDX4 gene.
REFERENCES
1. Abdelhaleem, M: Clin. Biochem. 38:499-503,2005
2. Noce, T. et al: Cell Struct. Funct. 26:131-6, 2001
3. Xu, H. et al: Dev. Dyn. 233:872-82, 2005
2. Noce, T. et al: Cell Struct. Funct. 26:131-6, 2001
3. Xu, H. et al: Dev. Dyn. 233:872-82, 2005
Products are for research use only. They are not intended for human, animal, or diagnostic applications.
(Click to Enlarge)
Top: Western Blot detection of DDX4 proteins in HEK293 cell lysate using DDX4 Antibody. Middle, upper: This antibody stains paraffin-embedded human lung cancer tissue in immunohistochemical analysis. Middle, lower: It also stains MSCS cells in confocal immunofluorescent testing (DDX4 antibody: Green; Actin filament: Red). Bottom: This antibody detects DDX4 proteins specifically in MSCS cells (Green) vs. normal mouse IgG control (Purple) by FACS assay.
Details
Cat.No.: | CP10069 |
Antigen: | Purified recombinant human DDX4 fragment-hIgGFc fusion expressed in HEK293 cells. |
Isotype: | Mouse IgG1 |
Species & predicted species cross- reactivity ( ): | Human, Mouse |
Applications & Suggested starting dilutions:* | WB 1:1000 IP n/d IHC 1:200 ICC 1:200 FACS 1:200 |
Predicted Molecular Weight of protein: | 76 kDa |
Specificity/Sensitivity: | Detects endogenous DDX4 proteins without cross-reactivity with other family members. |
Storage: | Store at -20°C, 4°C for frequent use. Avoid repeated freeze-thaw cycles. |
*Optimal working dilutions must be determined by end user.
Products
| Product | Size | CAT.# | Price | Quantity |
|---|---|---|---|---|
| Mouse DEAD Box Polypeptide 4 Antibody: Mouse DEAD Box Polypeptide 4 Antibody | Size: 100 ul | CAT.#: CP10069 | Price: $302.00 |