Product Sheet CP10029

Description

BACKGROUND Bruton\'s Tyrosine Kinase (BTK) is member of the Tec family that is critically important for the growth, differentiation and activation of myeloid-, mast- and B-cells.1 BTK is activated firstly by membrane localization stimulated by PIP3 generation, and subsequently, by transphosphorylation of Tyr-551 by Src family kinases. Further activation occurs within the SH3 domain via a transphosphorylation mechanism. Tyr223 in this domain was phosphorylated by c-Abl.2 Activated BTK is involved in the phosphorylation of a number of signaling molecules involved in the PLCgamma, JNK and p38 MAPK pathways, leading to Ca2+ mobilization, mRNA stabilization and the induction of NF-kappaB and AP-1 transcription factors.3 BTK activity is negatively regulated by a number of proteins including inhibitor of BTK (IBTK), Sab and c-Cbl. Mutations in this enzyme are known in humans and result in the immunological disorder X-linked agammaglobulemia.4
 
REFERENCES  
1. Mohamed, A.J. et al: Immunol. Rev. 228:58-73, 2009
2. Backesjo, C.M. et al: Biochem. Biophy. Res. Commun. 299:510-5, 2002
3. Kurosaki, T & Hikida, M.: Immunol. Rev. 228:132-48, 2009
4. Toth, B. et al: Mol. Immunol. 46:2140-6, 2009
  
Products are for research use only. They are not intended for human, animal, or diagnostic applications.

(Click to Enlarge)

Top: Western Blot detection of BTK proteins in various cell lysates using BTK Antibody. Middle: This antibody stains paraffin-embedded human lymph node tissue in immunohistochemical analysis.  Bottom: Immunofluorescence analysis of Jurkat cells using BTK mouse antibody.

Details

Cat.No.:
CP10029
Antigen:
Purified recombinant human BTK protein fragments expressed in E. coli.
Isotype:
Mouse IgG1
Species & predicted
species cross-
reactivity ( ):
Human, Monkey
Applications &
Suggested starting
dilutions:*
WB                  1:500 - 1:2000
IP                    n/d
IHC                  1:200
ICC                  1:200
FACS               n/d
Predicted Molecular
Weight of protein:
77 kDa
Specificity/Sensitivity:
Detects endogenous BTK proteins in normal cell lysates without cross-reactivity with other family members.
Storage:
Store at -20°C, 4°C for frequent use. Avoid repeated freeze-thaw cycles.

*Optimal working dilutions must be determined by end user.

Products

Product Size CAT.# Price Quantity
Mouse Bruton's Tyrosine Kinase Antibody: Mouse Bruton's Tyrosine Kinase Antibody Size: 100 ul CAT.#: CP10029 Price: $302.00
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