Product Sheet CA1004

Description

BACKGROUND There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The first three are located together on chromosome 2, while the tissue non-specific form is located on chromosome 1. ALPL (alkaline phosphatase, liver/bone/kidney) is a membrane bound glycosylated enzyme that is not expressed in any particular tissue and is, therefore, referred to as the tissue-nonspecific form of the enzyme.1 The exact physiological function of the alkaline phosphatases is not known. A proposed function of this form of the enzyme is matrix mineralization; however, mice that lack a functional form of this enzyme show normal skeletal development. This enzyme has been linked directly to hypophosphatasia, a disorder that is characterized by hypercalcemia and includes skeletal defects. The character of this disorder can vary, however, depending on the specific mutation since this determines age of onset and severity of symptoms. Alternatively spliced transcript variants have been described.2
 
REFERENCES  
1. Harris, H.: Clinica Chimica Acta 186:133-50, 1990
2. Benham, F.J. et al: Dev. Biol. 88:279-87, 1981
 
Products are for research use only. They are not intended for human, animal, or diagnostic applications.
(Click to Enlarge) Top: Western Blot detection of APLP proteins in HeLa cell lysate using ALPL Antibody. Bottom: This antibody stains paraffin-embedded rat intestine tissue in immunohistochemical analysis.
 

Details

Cat.No.:
CA1004
Antigen:
Short peptide from human ALPL sequence.
Isotype:
Rabbit IgG
Species & predicted
species cross-
reactivity ( ):
Human, Rat
Applications &
Suggested starting
dilutions:*
WB                  1:1000
IP                    n/d
IHC                  1:200
ICC                  n/d
FACS               n/d
Predicted Molecular
Weight of protein:
80 kDa
Specificity/Sensitivity:
Detects endogenous levels of ALPL proteins without cross-reactivity with other family members.
Storage:
Store at -20°C, 4°C for frequent use. Avoid repeated freeze-thaw cycles.

*Optimal working dilutions must be determined by end user.

Products

Product Size CAT.# Price Quantity
Rabbit Alkaline Phosphatase Antibody: Rabbit Alkaline Phosphatase Antibody Size: 100 ul CAT.#: CA1004 Price: $302.00
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Resources/Documents