Abl2 is involved in homologous recombination, DNA repair or cell cycle progression mechanisms. This protein can interact with c-abl and response to oxidative stress. Like c-abl, Abl2 seems to have a role in apoptosis via phosphorilation of the apoptotic Siva1 protein, control of cell motility and morphogenesis by modifying F-actin structure. Both proteins are also required for maintenance of cortical dendreties in brain. Abl2 is important for development of nervous system and for brain function in adult tissue. Mice with disruption of Abl2 can develop normally but display behavioral disorders. Through its interactions with the cytoskeleton Abl2 plays a role in murine neurulation and it is required for adhesion-dependent neurite branching and synapse/dendrite stability. Abl2 has also a role in fibroblastic- and epithelial cell adhesion and migration. Abl2 shares a number of cellular regulatory functions with c-Abl. Targeted disruption of the c-Abl gene in mice resulted in pleiotropic phenotypes including runtedness, high perinatal lethality, susceptibility to infections, and immune deficiencies. In contrast, homozygous Abl2 knockout mice are healthy and exhibit no immune-deficient phenotypes. Moreover, it was demonstrated that Abl2 and c-abl are differently expressed in B lymphoid cells at different stages of differentiation.2 Unlike Abl2, the level of c-abl transcripts was not related to differentiation. The highest level of Abl2 was found in mature B lymphoid cells (Raji cell line) in contrast to early pre B lymphoid cells. The increment of Abl2 level was also reported in other studies on granulocytes, monocytes and neuron. These findings support the hypothesis that high level of Abl2 is associated to the mature state of specific cells. However, evidence for overlapping roles of c-Abl and Abl2 was obtained by analyzing mice deficient for both genes, which revealed that both proteins are required for normal mouse development. Abl/Abl2-/- mice die around embryonic day 10.5, and the embryos display bleeding in the pericardial sac, exhibit reduced proliferation and cytokine production in response to TCR stimulation and showed a significantly impaired antibody production. Like c-Abl, Abl2 is involved in human neoplastic diseases. Abl is up- or down-regulated in several solid tumors and oncogenic gene translocations involving the ETV6 gene have been described in human acute leukemia.3
2. Shimizu, A. et al: J. Biol. Chem. 283:27230-8, 2008
3. Iijima, Y. et al: Blood 95: 2126-31, 2000
Purified recombinant human Abl2 fragments expressed in E. coli.
Species & predicted
reactivity ( ):
Human, Mouse, Rat
ICC 1:50 - 1:200
Weight of protein:
Detects Abl2 proteins without cross-reactivity with other related proteins.
Store at -20°C, 4°C for frequent use. Avoid repeated freeze-thaw cycles.
*Optimal working dilutions must be determined by end user.
|Mouse Abl2 Antibody: Mouse Abl2 Antibody||Size: 100 ul||CAT.#: CP10252||Price: $333.00|