Human iPSC-Derived Hepatic Stellate Cells: i-HHSC

Induced human hepatic stellate cells (i-HHSC), derived from induced pluripotent stem cells (iPSC), serve as a powerful tool for studying liver diseases. Hepatic stellate cells are located between hepatocytes and blood vessels in the liver, where they play crucial roles in liver homeostasis, maintenance, and regeneration. In the quiescent state, i-HHSC primarily function in the storage of retinoids within lipid droplets. However, upon activation, these cells expel lipid droplets and shift toward a proliferative state. The activation of hepatic stellate cells in various disease states contributes to the deposition of a collagen-rich extracellular matrix, a hallmark of liver fibrosis, and conditions such as hepatitis B and C, fatty liver disease, diabetes, and other chronic liver disorders.

i-HHSC are cultured in an optimized Hepatic Stellate Cell Growth Medium. When necessary, these cells can be transitioned into the quiescent state using Hepatic Stellate Cell Quiescent Medium, promoting the accumulation of lipid droplets. The culture of i-HHSC provides a valuable platform for studying liver function, physiology, and the pathophysiology of liver diseases.