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Human Epidermal Keratinocytes (HEK) provide an excellent model to study epithelial function and disease, skin biology, toxicology and HPV infection. When grown on inserts and provided a liquid/air interface, they differentiate into a stratified squamous epithelium, forming a physiological 3D tissue. HEK comprise 90 % of all cells in the epidermis, the outermost skin layer. They produce a barrier, the intermediate filament keratin. Keratinocytes obtain melanin from melanocytes (HEM) and store it as protection from UV radiation. We highly recommend optimized Human EpiVita Media to enhance the performance and in vitro lifespan of HEK.
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HEK from Cell Applications, Inc. have been utilized in numerous research publications, for example in basic research to investigate mechanisms of cell senescence and develop ways to extend cell life span. Others have used HEK to design a method to isolate proteins for use in basic researh, and show higher relevance of primary keratinocytes when compared to key differences exhibited by immortalized keratinocyte cell lines. Pharmaceutical companies employ HEK to provide in vitro human skin models that assess effects of cosmetic formulations or anti-inflammatory properties of proposed anti-psoriasis drugs. Translational research on keratinocytes benefits from their ability to model the wound healing response, or help develop serum tests for patients with blistering diseases. HEK are also utilized to understand aggressiveness of cancer phenotypes, investigate compounds for potential topical melanoma treatments, and even develop siRNA therapy targeting oncogenes. In addition, HEK help unravel the oncogenic potential of HPV and test approaches for treating HPV lesions.
Normal healthy human foreskin or adult skin
No bacteria, yeast, fungi, mycoplasma, virus
Attach, spread, proliferate in Growth Med
500,000 HEK (Primary culture) frozen in Basal Medium w/ 10% FBS, 10% DMSO
Cryovial HEK (102-05), Epi-Vita Growth Medium (141-500a), Subcltr Rgnt Kit (090K)
In Serum-Fr Gr Med, psg 1, flasks or plates
At least 16 in keratinocyte media
Laboratory research use only (RUO). Not for human, clinical, diagnostic or veterinary use.
|Cryopreserved HEK Total Kit: 5x10^5 Cells (Adult), Medium & Subculture Reagents (See Details tab for specifics)||Size: 1 Kit||CAT.#: 102K-05a||Price: $549.86|
|Cryopreserved HEK Total Kit: 5x10^5 Cells (Fetal), Medium & Subculture Reagents (See Details tab for specifics)||Size: 1 Kit||CAT.#: 102K-05f||Price: $568.74|
|Cryopreserved HEK Total Kit: 5x10^5 Cells (Neonatal), Medium & Subculture Reagents (See Details tab for specifics)||Size: 1 Kit||CAT.#: 102K-05n||Price: $563.50|
|Cryopreserved HEK, adult: Frozen HEK (5x10^5)||Size: 1 Cryovial||CAT.#: 102-05a||Price: $392.83|
|Cryopreserved HEK, fetal: Frozen HEK (5x10^5)||Size: 1 Cryovial||CAT.#: 102-05f||Price: $414.36|
|Cryopreserved HEK, neonatal: Frozen HEK (5x10^5)||Size: 1 Cryovial||CAT.#: 102-05n||Price: $408.98|
|Proliferating HEK, adult: Actively growing, dividing cells, in medium||Size: T-25 Flask||CAT.#: 103-25a||Price: $392.83|
|Proliferating HEK, adult: Actively growing, dividing cells, in medium||Size: T-75 Flask||CAT.#: 103-75a||Price: $597.32|
|Proliferating HEK, adult: Actively growing, dividing cells, in medium||Size: 6 Well||CAT.#: 103-6Wa||Price: $597.32|
|Proliferating HEK, adult: Actively growing, dividing cells, in medium||Size: 96 Well||CAT.#: 103-96Wa||Price: $729.70|
|Proliferating HEK, fetal: Actively growing, dividing cells, in medium||Size: T-25 Flask||CAT.#: 103-25f||Price: $511.22|
|Proliferating HEK, fetal: Actively growing, dividing cells, in medium||Size: T-75 Flask||CAT.#: 103-75f||Price: $715.71|
|Proliferating HEK, fetal: Actively growing, dividing cells, in medium||Size: 6 Well||CAT.#: 103-6Wf||Price: $715.71|
|Proliferating HEK, fetal: Actively growing, dividing cells, in medium||Size: 96 Well||CAT.#: 103-96Wf||Price: $844.86|
|Proliferating HEK, neonatal: Actively growing, dividing cells, in medium||Size: T-25 Flask||CAT.#: 103-25n||Price: $408.98|
|Proliferating HEK, neonatal: Actively growing, dividing cells, in medium||Size: T-75 Flask||CAT.#: 103-75n||Price: $613.46|
|Proliferating HEK, neonatal: Actively growing, dividing cells, in medium||Size: 6 Well||CAT.#: 103-6Wn||Price: $613.46|
|Proliferating HEK, neonatal: Actively growing, dividing cells, in medium||Size: 96 Well||CAT.#: 103-96Wn||Price: $742.61|
|Human EpiVita Basal Medium: Basal medium (contains no growth supplement). Add GS before use.||Size: 500 ml||CAT.#: 140-500||Price: $74.26|
|Human EpiVita Defined Growth Medium: All-in-one ready-to-use||Size: 500||CAT.#: 141DGM-500||Price: $105.47|
|Human EpiVita Defined Growth Medium Kit: Basal medium & growth supplement sold together packaged separately||Size: Yields 500 ml||CAT.#: 141DGMK-500||Price: $116.24|
|Human EpiVita Defined Growth Supplement: Added to Basal Medium to create Growth Medium||Size: 5 ml||CAT.#: 141DGM-GS||Price: $47.36|
|Human EpiVita Serum Free Growth Medium: All-in-one ready-to-use, standard Epithelial Cell Medium, for neonatal and fetal cells||Size: 500||CAT.#: 141-500||Price: $103.32|
|Human EpiVita Serum Free Growth Medium: All-in-one ready-to-use, standard Epithelial Cell Medium, for adult cells||Size: 500||CAT.#: 141-500a||Price: $105.47|
|Human EpiVita Serum Free Growth Medium Kit: Basal medium & growth supplement sold together packaged separately, for neonatal and fetal cells||Size: Yields 500 ml||CAT.#: 141K-500||Price: $114.08|
|Human EpiVita Serum Free Growth Medium Kit: Basal medium & growth supplement sold together packaged separately, for adult cells||Size: Yields 500 ml||CAT.#: 141K-500a||Price: $116.24|
|Human EpiVita Serum Free Growth Supplement: Added to Basal Medium to create Growth Medium, for neonatal and fetal cells||Size: 5 ml||CAT.#: 141-GS||Price: $45.20|
|Human EpiVita Serum Free Growth Supplement: Added to Basal Medium to create Growth Medium, for adult cells||Size: 5 ml||CAT.#: 141-GSa||Price: $47.36|
Extended Family Products
|3D Skin Model: HEK differentiated into stratified squamous epithelium on PCF inserts||Size: 12 Inserts||CAT.#: 102-3D-12||Price: $640.37|
|3D Skin Model: HEK differentiated into stratified squamous epithelium on PCF inserts||Size: 24 Inserts||CAT.#: 102-3D-24||Price: $1,183.88|
|3D Skin Model Total Kit: Cells, Media, Reagents & Inserts (See Details tab for specifics)||Size: 1 Kit||CAT.#: 102K-3D||Price: $640.37|
|Anti-CXCR1: Polyclonal CXCR1 Rabbit Antibody||Size: 100 ul||CAT.#: CA0755||Price: $302.38|
|Anti-CXCR2: Polyclonal CXCR2 Rabbit Antibody||Size: 100 ul||CAT.#: CA0757||Price: $302.38|
|Cyto-X Colorimetric Cell Counting Reagent: 500 tests||Size: 1 Bottle||CAT.#: 028-01||Price: $138.84|
|Cyto-X Colorimetric Cell Counting Reagent: 100 tests||Size: Sample||CAT.#: 028-S||Price: $35.88|
|EGF: Human Epidermal Growth Factor||Size: 100 ug||CAT.#: RP1026-100||Price: $86.10|
|EGF: Human Epidermal Growth Factor||Size: 500 ug||CAT.#: RP1026-500||Price: $193.73|
|EGF: Human Epidermal Growth Factor||Size: 1000 ug||CAT.#: RP1026-1000||Price: $263.68|
|EGF, Animal-Free: Human Epidermal Growth Factor, Animal-Free||Size: 100 ug||CAT.#: RP1026AF-100||Price: $94.71|
|EGF, Animal-Free: Human Epidermal Growth Factor, Animal-Free||Size: 500 ug||CAT.#: RP1026AF-500||Price: $213.10|
|EGF, Animal-Free: Human Epidermal Growth Factor, Animal-Free||Size: 1000 ug||CAT.#: RP1026AF-1000||Price: $290.05|
|HEK RNA: Total RNA prepared from Human Epidermal Keratinocytes, adult||Size: 10 ug||CAT.#: 102-R10a||Price: $349.78|
|HEK RNA: Total RNA prepared from Human Epidermal Keratinocytes, adult||Size: 25 ug||CAT.#: 102-R25a||Price: $699.56|
|HEK RNA: Total RNA prepared from Human Epidermal Keratinocytes, neonatal||Size: 10 ug||CAT.#: 102-R10n||Price: $349.78|
|HEK RNA: Total RNA prepared from Human Epidermal Keratinocytes, neonatal||Size: 25 ug||CAT.#: 102-R25n||Price: $699.56|
|HEK RNA: Total RNA prepared from Human Epidermal Keratinocytes, fetal||Size: 10 ug||CAT.#: 102-R10f||Price: $349.78|
|HEK RNA: Total RNA prepared from Human Epidermal Keratinocytes, fetal||Size: 25 ug||CAT.#: 102-R25f||Price: $699.56|
|Subculture Reagent Kit: 100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution||Size: 1 Kit||CAT.#: 090K||Price: $54.89|
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