Instructions 022K DiI-Ac-LDL

Description

DiI-Ac-LDL Kit:  Acetelyated (Ac), fluorescently-labeled low-density lipoprotein (LDL) binds to a receptor on the surface of vascular endothelial cells & macrophages, delivering cholesterol via endocytosis.  This simple, one-step process permits cultured cells to be identified and analyzed by fluorescence microscopy or flow cytometry, and to be isolated from mixed cell populations. Because contaminating cell types are not labeled, pure cultures of vascular endothelial cells or macrophages can be isolated from complex primary cultures using fluorescent activated cell sorting (FACS).  DiI Labeled Cells Remain Viable and can be Trypsinized.
(Click to Enlarge) Cryopreserved Rat Macrophages: RMcp (top) cultured for 2 days. Bottom: DiI-Ac-LDL (in red) uptake in cryopreserved RMcp cultured for 3 days (R).  Cryopreserved RMcp cultured for 4 days stained for CD11b/c (in green) and DAPI (in blue) (R).

Products

Product Size CAT.# Price Quantity
DiI-Ac-LDL Kit: DiI-conjugated acetylated low-density lipoproteins can be used to identify endothelial cells. (One week lead time required) Size: 1 Kit CAT.#: 022K Price: $161.00
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