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Human Subclavian Artery Smooth Muscle Cells: HScASMC



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Human Subclavian Artery Smooth Muscle Cells, HScASMC

Human subclavian artery smooth muscle cells (HScASMC ) are derived from the subclavian artery. They are cryopreserved at second passage. HScASMC can be cultured and propagated 12 population doublings. Morphology and growth characteristics of HScASMC from neonates were found to be different from those of adults1. A rabbit model using the subclavian artery was developed to study the temporal development and characteristics of an intimal cellular response and functional changes following balloon angioplasty injury2. Prolonged treatment with r-hirudin neither inhibits vascular smooth muscle cell proliferation in rabbits after angioplasty of the left subclavian artery nor reduces the size of neointimal lesion. This suggests r-hirudin treatment is not suitable as an antirestenotic intervention3. Subclavian-pulmonary artery anastomoses were created in Sprague-Dawley rats under different experimental conditions. It was found that the type of injury and the associated hemodynamic conditions could modify the elastic pulmonary response to injury4.
  1. Fujita H. et al, Exp Mol. Pathol. 1993 Feb; 58(1):25-39.
  2. Hadoke P, et al, Coron Artery Dis. 1995 May; 6(5):403-15.
  3. Hadoke PW, et al, Coron Artery Dis. 1996 Aug; 7(8):599-608.
  4. Tanaka Y, et al, J Clin Invest. 1996 Jul 15; 98(2):434-42.  
Each lot tested negative for HIV, Hepatitis B and Hepatitis C and negative for mycoplasma, bacteria, yeast, fungi.
Products are for research use only. They are not intended for human, animal, or diagnostic applications.

Tissue:
Normal human subclavian artery
Cryopreserved ampoule:
2nd passage, >500,000 cells in Basal Medium containing 10% FBS & 10% DMSO.
Kit contains:
Ampoule of cryopreserved HScASMC (3510-05a), 500 ml of Smooth Muscle Cell Growth Media (311-500), and a Subculture Reagent Kit (090K).
Proliferating Cells:
 
Shipped in Growth Medium at 3rd passage in either flasks or multiwell dishes.
Population doublings:
Can be cultured at least 12 doublings

Call 800-645-0848 or 858-453-0848 to place an order.
Cell Products
Product Name Product Description Size Cat. No. Price
Cryopreserved HScASMC, adult Each kit contains an ampoule of cryopreserved HScASMC (3510-05a), 500 ml of Smooth Muscle Cell Growth Medium (311-500), and a Subculture Reagent Kit (090K). 1 Kit
1 Ampoule 		3510K-05a
3510-05a 		$797
$659
Proliferating HScASMC, adult Cultured and shipped in flasks at third passage in Smooth Muscle Cell Growth Medium. T-25 Flask
T-75 Flask
6 well
96 well 		3511-25a
3511-75a
3511-6Wa
3511-96Wa 		$659
$849
$849
$969
 
Media and Reagents
Product Name Product Description Size Cat. No. Price
Smooth Muscle Cell Growth Medium Optimized and ready for use 500 ml 311-500 $98
Smooth Muscle Cell Growth Medium Kit Basal Medium & Growth Supplement packaged separately Yields 500 ml 311K-500 $105
Smooth Muscle Cell Basal Medium Add Growth Supplement before use 500 ml 310-500 $53
Smooth Muscle Cell Growth Supplement Added to Basal Medium to create Growth Medium 30 ml 311-GS $53
Smooth Muscle Cell Differentiation Medium    250 ml 311D-250 $68
Subculture Reagent Kit 100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution 100 ml 090K $51