Main Page >> >> Human Brachiocephalic Artery Smooth Muscle Cells: HBcASMC
Human Brachiocephalic Artery Smooth Muscle Cells: HBcASMC



(Enlarge Image)

Human Brachiocephalic Artery Smooth Muscle Cells, HBcASMC

Human Brachiocephalic Artery Smooth Muscle Cells (HBcASMC) are primary smooth muscle cells isolated from normal brachiocephalic arteries and are cryopreserved at third passage. HBcASMC can be propagated at least 12 doublings. The brachiocephalic artery is the first branchfrom the aortic arch. HBcASMC provide an in vitro system to explore in vivo findings. Apolipoprotein E (apoE) knockout mice serve as a model for atherosclerotic lesion development, as fibrous plaques commonly form in the brachiocephalic artery after 6 months of a high-fat diet, thus providing a small well-defined area in which to study the progressive loss of plaque stability and eventual rupture1,2,3. p53 protects brachiocephalic and aortic smooth muscle cells against apoptosis while promoting apoptosis in macrophages, thus protecting against atherosclerosis development4. Reduced blood flow during pregnancy is associated with hypotrophy of the endothelial and smooth muscle cells of the carotid artery in the guinea pig5.

  1.  Williams, H et al., Arterioscler Thromb Vasc Biol. 22(5):788 (2002).
  2. Rosenfeld, ME et al., Cur Atheroscler Rep. 4(3):238 (2002).
  3. Johnson, JL & Jackson, CL. Atherosclerosis. 154(2):399 (2001).
  4. Mercer, J et al., Circ Res. 96(6):667 (2005).
  5. Jovanovic, S & Jovanovic, A. Hum Reprod. 13(4):1074 (1998).

Each lot tested negative for HIV, Hepatitis B and Hepatitis C and negative for mycoplasma, bacteria, yeast, fungi.
Products are for research use only. They are not intended for human, animal, or diagnostic applications.

Tissue:
Human normal brachiocephalic artery
Cryopreserved ampoule:
2nd passage, >500,000 cells in Basal Medium containing 10% FBS & 10% DMSO.
Kit contains:
Ampoule of cryopreserved HBcASMC (3512-05a), 500 ml of Smooth Muscle Cell Growth Medium (311-500), and a Subculture Reagent Kit (090K).
Proliferating Cells:
 
Shipped in Smooth Musce Cell Growth Medium at 3rd passage in either flasks or multiwell dishes.
Population doublings:
Can be cultured at least 12 doublings

Call 800-645-0848 or 858-453-0848 to place an order.
Cell Products
Product Name Product Description Size Cat. No. Price
Cryopreserved HBcASMC, adult Each kit contains an ampoule of cryopreserved HBcASMC (3512-05a), 500 ml of Smooth Muscle Cell Growth Medium (311-500), and a Subculture Reagent Kit (090K). 1 Kit
1 Ampoule 		3512K-05a
3512-05a 		$797
$659
Proliferating HBcASMC, adult Cultured and shipped at third passage in Smooth Muscle Cell Growth. T-25 flask
T-75 flask
6 well
96 well 		3513-25a
3515-75a
3513-6Wa
3515-96Wa 		$659
$849
$849
$969
 
Media and Reagents
Product Name Product Description Size Cat. No. Price
Smooth Muscle Cell Growth Medium Optimized and ready for use 500 ml 311-500 $98
Smooth Muscle Cell Growth Medium Kit Basal Medium & Growth Supplement packaged separately Yields 500ml 311K-500 $105
Smooth Muscle Cell Basal Medium Add Growth Supplements before use 500 ml 310-500 $53
Smooth Muscle Cell Growth Supplement Added to Basal Medium to create Growth Medium 30 ml 311-GS $53
Subculture Reagent Kit 100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution 100 ml 090K $51