BACKGROUND Transfection, the introduction of DNA into cells, is a powerful technique used to study in vivo gene function and regulation. The Cytofect Smooth Muscle Cell Transfection kit is a plasmid DNA delivery system specifically optimized to deliver DNA into a wide variety of smooth muscle cells. This kit contains Cytofect-2 and Media for transfection and culturing cells. The result has been high transfection efficiency and viability for these traditionally hard-to-transfect primary smooth muscle cells.
This Cytofect Smooth Muscle Cell Transfection Kit contains all the necessary reagents and media for transfection. Optimal transfection protocols are provided in the kit to carry out successful transfection of the following smooth muscle cells with high efficiency (40%-90%) and viability (80%-95%):
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HAOSMC: Human Aortic Smooth Muscle Cells
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HCASMC: Human Coronary Artery Smooth Muscle Cells
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HPASMC: Human Pulmonary Artery Smooth Muscle Cells
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HITASMC: Human Internal Thoracic Artery Smooth Muscle Cells
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HCtASMC: Human Carotid Artery Smooth Muscle Cells
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HBcASMC: Brachiocephalic Artery Smooth Muscle Cells
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HScASMC: Human Subclavian Artery Smooth Muscle Cells
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HUVSMC: Human Umbilical Vein Smooth Muscle Cells
Products are for research use only. They are not intended for human, animal, or diagnostic applications. CF1 is manufactured by Targeting Systems.
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