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PPAEC: Porcine Pulmonary Artery Endothelial Cells



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Porcine Pulmonary Artery Endothelial Cells, PPAEC

Porcine Pulmonary Artery Endothelial Cells (PPAEC) are derived from healthly, plaque free porcine pulmonary arteries. PPAEC are cryopreserved at first passage and can be cultured and propagated at least 16 population doublings. Arterial endothelial cells play critical roles in cardiac homeostasis. Insults to the endothelial cells have been linked to a variety of vascular diseases. Porcine artery endothelial cells have been used as a viable alternative for the study of artery endothelial function and metabolism, such as atherosclerosis1, relaxing factor synthesis2, antioxidant enzyme activities3, etc. Co-culture of the artery endothelial cells with species-matched smooth muscle cells provides an ideal model for studying the interaction between these two cell types.
  1. Ross, R.N., Nature 362:801 (1993).
  2. Boulanger, C.M. et al, J. fo Vasc. Res. 34(3):175 (1997).
  3. Bishop, C.T. et al, In Vitro Cell Devel. Biol. 21:229 (1985).
Each lot tested negative for HIV, Hepatitis B and Hepatitis C and negative for mycoplasma, bacteria, yeast, fungi.
Products are for research use only. They are not intended for human, animal, or diagnostic applications.

Tissue:
Normal porcine aorta
Cryopreserved ampoule:
1st passage, >500,000 cells in Porcine Endothelial Cell Growth Medium that contains 10% FBS and 10% DMSO.
Kit contains:
Ampoule of cryopreserved PPAEC (P302-05), 500 ml of Porcine Endothelial Cell Growth Medium (P211-500), and a Subculture Reagent Kit (090K).
Proliferating Cells:
 
Shipped in Porcine Endothelial Cell Growth Medium at 2nd passage in either flasks or multiwell dishes.
Population doublings:
Can be cultured at least 16 doublings

Call 800-645-0848 or 858-453-0848 to place an order.
Cell Products
Product Name Product Description Size Cat. No. Price
Cryopreserved PPAEC Each kit contains an ampoule of >500,000 cryopreserved cells, 500 ml of Porcine Endothelial Cell Growth Medium (P211-500), and a Subculture Reagent Kit (090K). 1 Kit
1 Ampoule 		P302K-05
P302-05 		$674
$535
Proliferating PPAEC Cultured and shipped at second passage in Porcine Endothelial Cell Growth Medium. T-25 Flask
T-75 Flask
6 well
96 well 		P303-25
P303-75
P303-6W
P303-96W 		$535
$725
$725
$845
 
Media and Reagents
Product Name Product Description Size Cat. No. Price
Porcine Endothelial Cell Growth Medium Optimized and ready for use 500 ml P211-500 $98
Porcine Endothelial Cell Growth Medium Kit Basal Medium & Growth Supplement packaged separately Yields 500 ml P211K-500 $105
Porcine Endothelial Cell Basal Medium Add Growth Supplement before use 500 ml P210-500 $53
Porcine Endothelial Cell Growth Supplement Added to Basal Medium to create Growth Medium 50 ml P211-GS $53
Subculture Reagent Kit 100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution 100 ml 090K $51