Human Dermal Lymphatic Microvascular Endothelial (HDLMVEC) are isolated from lymphatic capillaries of normal human dermis. They are cryopreserved at second passage and can be cultured and propagated 12 population doublings. The lymphatic system interacts closely with the blood vascular system in maintaining tissue homeostasis. It plays a vital role in the body by regulating the immune system against infection.1 returning interstitial fluid and protein back to the blood circulation2 and absorbing and transport fat to the circulatory system.3 Although lymphatic micorvascular endothelial cells have many properties in common with blood microvascular endothelial cells, they also have unique functions due to their structural features. Lymphatic capillaries have thin and discontinuous base membrane4 and are directly connect to the insterstitial collagens by elastic anchoring filaments.5 The endothelial cells of lymphatic vessel wall loosely overlap to form easily opened minivalves that make them highly permeable. Recent studies demonstrate that tumor lymphangiogenesis is a major component of the metastatic process6 and members of the VEGF family are key mediators of lymphangiogenesis in tumors.7
1. Yoffey J. M. et al, Lymphatics, Lymph and the Lymphomyeloid Complex (Academic, London) (1970)
2. Rusznyak I. et al, Lymphatics and Lymph Circulation (Pergamon, Oxford) (1967)
3. Witte M. H. et al, Microsc Res Tech 55:122–145 (2001)
4. Springer, T. Cell 76:301 (1994)
5. Gerli R. et al, Anat Embryol 181(3):281(1990)
6. Swartz, M. A. et al, Microsc Res Tech 55:92 (2001)
7. Kabashima, A. et al, Oncology 60:146 (2001)
Each lot tested negative for HIV, Hepatitis B and Hepatitis C and negative for mycoplasma, bacteria, yeast, fungi.
Products are for research use only. They are not intended for human, animal, or diagnostic applications.