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Human Dermal Lymphatic Microvascular Endothelial Cells: HDLMVEC



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Left: Human Dermal Lymphatic Microvascular Endothelial Cells. Right: Human Dermal Lymphatic Microvascular Endothelial Cells (HDLMVEC) were stimulated with VEGF-C (50ng/ml for 5 min). VEGF-C stimulated HDLMVEC lysate was subjected to Western Blot analysis using Phospho-VEGFR3 (Tyr1063/1068) antibody (Top) and VEGFR3 antibody (Cat# CB5792) (Bottom). The results showed that HDLMVEC expressed VEGFR3, and that VEGFR3 can be activated by VEGF-C.

Human Dermal Lymphatic Microvascular Endothelial (HDLMVEC) are isolated from lymphatic capillaries of normal human dermis. They are cryopreserved at second passage and can be cultured and propagated 12 population doublings. The lymphatic system interacts closely with the blood vascular system in maintaining tissue homeostasis. It plays a vital role in the body by regulating the immune system against infection.1 returning interstitial fluid and protein back to the blood circulation2 and absorbing and transport fat to the circulatory system.3 Although lymphatic micorvascular endothelial cells have many properties in common with blood microvascular endothelial cells, they also have unique functions due to their structural features. Lymphatic capillaries have thin and discontinuous base membrane4 and are directly connect to the insterstitial collagens by elastic anchoring filaments.5 The endothelial cells of lymphatic vessel wall loosely overlap to form easily opened minivalves that make them highly permeable. Recent studies demonstrate that tumor lymphangiogenesis is a major component of the metastatic process6 and members of the VEGF family are key mediators of lymphangiogenesis in tumors.7
 
1. Yoffey J. M. et al, Lymphatics, Lymph and the Lymphomyeloid Complex (Academic, London) (1970)
2. Rusznyak I. et al, Lymphatics and Lymph Circulation (Pergamon, Oxford) (1967)
3. Witte M. H. et al,  Microsc Res Tech 55:122–145 (2001)
4. Springer, T. Cell 76:301 (1994)
5. Gerli R. et al, Anat Embryol 181(3):281(1990)
6. Swartz, M. A. et al, Microsc Res Tech 55:92 (2001)
7. Kabashima, A. et al, Oncology 60:146 (2001) 
 
 
Each lot tested negative for HIV, Hepatitis B and Hepatitis C and negative for mycoplasma, bacteria, yeast, fungi.
Products are for research use only. They are not intended for human, animal, or diagnostic applications.

Tissue:
Human lymphatic capillaries of normal human dermis
Cryopreserved ampoule:
3rd passage, >500,000 cells in Cell Basal Medium containing 10% FBS & 10% DMSO.
Kit contains:
An ampoule of cryopreserved HDLMVEC (100L-05a), 500 ml of Microvascular Endothelial Cell Growth Medium (111-500), Attachment Factor Solution (123-100), and a Subculture Reagent Kit (090K).
Proliferating Cells:
 
Shipped in Transfer Media at 4th passage in either flasks or multiwell dishes.
Population doublings:
Can be cultured at least 12 doublings

Call 800-645-0848 or 858-453-0848 to place an order.
Cell Products
Product Name Product Description Size Cat. No. Price
Cryopreserved HDLMVEC, adult Each kit contains an ampoule of cryopreserved HDLMVEC (100L-05), 500 ml of Microvascular Endothelial Cell Growth Medium (111-500), 100 ml of Attachment Factor Solution (123-100), a Subculture Reagent Kit. 1 Kit
1 Ampoule 		100LK-05a
100L-05a 		$857
$650
Proliferating HDLMVEC, adult Cultured at fourth passage in Microvascular Endothelial Cell Growth Medium and shipped in Endothelial Cell Transfer Medium. T-25 Flask
T-75 Flask
6 well
96 well 		101L-25a
101L-75a
101L-6Wa
101L-96Wa 		$650
$840
$840
$960
 
Media and Reagents
Product Name Product Description Size Cat. No. Price
Microvascular Endothelial Cell Growth Medium Optimized and ready for use 500 ml 111-500 $120
Microvascular Endothelial Cell Growth Medium Kit Basal Medium & Growth Supplement packaged separately Yields 500 ml 111K-500 $128
Microvascular Endothelial Cell Basal Medium Add Growth Supplement before use 500 ml 100-500 $53
Microvascular Endothelial Cell Growth Supplement Added to Basal Medium to create Growth Medium 50 ml 111-GS $75
Endothelial Cell Serum-Free Defined Medium For maintaining the monolayer of endothelial cells at 100% confluent culture. Does not contain VEGF, FGF, PDGF. It is supplemented with defined components to allow metabolic and growth factor studies without interference of extraneous factors. Available without Phenol Red (113PR-500). 500 ml
500 ml 		113-500
113PR-500 		$105
$110
Attachment Factor Solution Promotes attachment of endothelial cells. 100 ml 123-100 $18
Subculture Reagent Kit 100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution 100 ml 090K $51