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BRASMC: Bovine Renal Artery Smooth Muscle Cells



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Bovine Renal Artery Smooth Muscle Cells, BRASMC

Bovine Renal Artery Smooth Muscle Cells (BRASMC) are derived from tunica intima and tunica media of normal bovine renal arteries. They are cryopreserved at second passage and can be cultured and propagated at least 12 population doublings. Studies show cyclosporine-induced renal artery smooth muscle contraction is associated with increases in the phosphorylation of specific contractile regulatory proteins1. The renal artery smooth muscle is uniquely refractory to angiotensin stimulation and may be protective in the role of the kidney in regulating blood pressure through the renin-angiotensin system2.
  1. Beall, A. et al, Biochem Biophys Acta., 1449(1):41-9 (1999).
  2. Whitney, E.G. et al, J. Surg. Res. 61(2):307-10 (1996).
Products are for research use only. They are not intended for human, animal, or diagnostic application.
Animal tissues were USDA inspected. 

Tissue:
Tunica intima and media of normal bovine renal artery
Cryopreserved ampoule:
2nd passage, >500,000 cells in Bovine Smooth Muscle Cell Basal Medium containing 10% FBS & 10% DMSO.
Kit contains:
Ampoule of cryopreserved BRASMC (B950-05), 500 ml Bovine Smooth Muscle Cell Growth Medium (B311-500), and a Subculture Reagent Kit (090K).
Proliferating Cells:
 
Shipped in Bovine Smooth Muscle Cell Growth Medium at 3rd passage in either flasks or multiwell dishes.
Population doublings:
Can be cultured at least 12 doublings

Call 800-645-0848 or 858-453-0848 to place an order.
Cell Products
Product Name Product Description Size Cat. No. Price
Cryopreserved BRASMC Each kit contains an ampoule of >500,000 cryopreserved cells, 500 ml of Bovine Smooth Muscle Cell Growth Medium (B311-500), and a Subculture Reagent Kit (090K). 1 Kit
1 Ampoule 		B950K-05
B950-05 		$714
$575
Proliferating BRASMC Cultured and shipped at third passage in Bovine Smooth Muscle Cell Growth Medium. T-25 Flask
T-75 Flask
6 well
96 well 		B951-25
B951-75
B951-6W
B951-96W 		$575
$765
$765
$885
 
Media and Reagents
Product Name Product Description Size Cat. No. Price
Bovine SMC Growth Medium Optimized and ready for use 500 ml B311-500 $98
Bovine SMC Growth Medium Kit Basal Medium & Growth Supplement packaged separately Yields 500 ml B311K-500 $105
Bovine SMC Basal Medium Add Growth Supplement before use 500 ml B310-500 $53
Bovine SMC Growth Supplement Added to Basal Medium to create Growth Medium 50 ml (2 parts) B311-GS $53
Bovine SMC Induction Medium Bovine Smooth Muscle Cell Induction Medium 250 ml B311I-250 $58
Subculture Reagent Kit 100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution 100 ml 090K $51