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BBMVEC: Bovine Brain Microvascular Endothelial Cells



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Bovine Brain Microvascular Endothelial Cells, BBMVEC

Bovine Brain Microvascular Endothelial Cells (BBMVEC) are isolated from microvessels of bovine brain. The cells are cryopreserved at second passage. BBMVEC can be cultured and propagated for at least 12 population doublings. Brain Microvascular Endothelial Cells form the basis of the blood-brain barrier, and have been used as a model for research on bacterial meningitis1,2,3. Hypoxia-ischemia studies showed that BBMVEC cell death may exacerbate ischemic brain injury4. BBMVEC have also been used to show oxyhemoglobin induces apoptosis in vascular endothelial cells5. Co-culture with Atrocytes generate an in vitro model that closely mimics the in vivo situation for evaluating drug transport6.
  1. Zysk, G. et al, Infect. Immun. 69(2):845-852 (2001)
  2. Prasadarao, N.V. et al, Infect. Immun. 67(3):1131-1138(1999) 
  3. Wang, Y. et al, Infect. Immun. 67(9):4751-4756 (1999)
  4. Zhang, J. et al, Brain Res. 877(2):134-140 (2000) 
  5. Meguro, T. et al, J. Neurochem. 77(4):1128-1135
  6. Cecchelli, R. et al, Adv. Drug Deliv Rev 36:165 (1999)
Products are for research use only. They are not intended for human, animal, or diagnostic application.
Animal tissues were USDA inspected. 

Tissue:
Bovine brain microvessels
Cryopreserved ampoule:
2nd passage, >500,000 cells in Bovine Brain Endothelial Cell Basal Medium containing 10% FBS & 10% DMSO.
Kit contains:
Ampoule of cryopreserved BBMVEC (B840-05), 500 ml Bovine Brain Endothelial Cell Growth Medium (B819-500), and a Subculture Reagent Kit (090K).
Proliferating Cells:
 
Shipped in Bovine Brain Endothelial Cell Growth Medium at 3rd passage in either flasks or multiwell dishes.
Population doublings:
Can be cultured at least 12 doublings

Call 800-645-0848 or 858-453-0848 to place an order.
Cell Products
Product Name Product Description Size Cat. No. Price
Cryopreserved BBMVEC Each kit contains an ampoule of >500,000 cryopreserved cells (B840-05), 500 ml of Bovine Brain Endothelial Cell Growth Medium (B819-500), and a Subculture Reagent Kit (090K). 1 Kit
1 Ampoule 		B840K-05
B840-05 		$832
$675
Proliferating BBMVEC Cultured and shipped in flasks at third passage in Bovine Brain Endothelial Cell Growth Medium. T-25 Flask
T-75 Flask
6 well
96 well 		B841-25
B841-75
B841-6W
B841-96W 		$675
$865
$865
$985
 
Media and Reagents
Product Name Product Description Size Cat. No. Price
Bovine Brain Endothelial Cell Growth Medium All-in-one ready-to-use media 500 ml B819-500 $98
Bovine Brain Endothelial Cell Growth Medium Kit Basal Medium & Growth Supplement packaged separately Yields 500 ml B819K-500 $105
Bovine Brain Endothelial Cell Basal Medium Add Growth Supplement before use 500 ml B818-500 $53
Bovine Brain Endothelial Cell Growth Supplement Added to Basal Medium to create Growth Medium 50 ml x 2 B819-GS $53
Subculture Reagent Kit 100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution 100 ml 090K $51
 
RNA
Product Name Product Description Size Cat. No. Price
BBMVEC RNA Total RNA prepared from BBMVEC 10 ug
25 ug 		B840-R10
B840-R25 		$380
$760