Instructions Gastrointestinal EpC
Cell Apps Flyer Epithelial Cells
5 Important Cell Culture Rules

Description

Ulcerative Colitis (UC) is a chronic inflammatory bowel disease (IBD) that causes inflammation and ulcers in the digestive tract. In contrast to Crohn’s Disease (see Treated CD and Untreated CD), which can affect any part of the GI and the entire thickness of the bowel wall, Ulcerative Colitis affects the innermost lining of the colon and rectum. Inflammation is patchy throughout the CD bowel, while inflammation is continuous and uniform in UC. Research suggests infection and abnormal immune response may trigger Ulcerative Colitis, and genetics and microbiome may be contributing factors, but mechanism of disease pathology is unclear. 

To better understand Ulcerative Colitis and disease treatment, Cell Applications offers researchers access to a first-of-kind biobank of UC Epithelial Cells. These Ulcerative Colitis cells originate from colon (Cn) and Rectum (Re) tissue samples, and includes different types of UC. Ulcerative Colitis is classified by location, and include: Proctitis (rectum), Proctosigmoiditis (rectum and sigmoid colon), Left-sided colitis (rectum, sigmoid, descending colon), and Pancolitis (entire colon).

For your convenience, the Epithelial Cell Total Kit includes all the reagents you need for gastrointestinal epithelial cell culture. Along with a cryovial of 500,000 diseased epithelial cells, the Total Kit includes optimized cell culture medium, thawing medium, and coating solution. Cells can be cultured up to 7 days. Cells cannot be passaged and should be plated directly into the experimental system. 

Learn more about:

Ulcerative Colitis Gastrointestinal Epithelial Cells from Naive sample (HCnEpC-UC-N): phase contrast 100x, immunostained for ZO-1 (red), Villin (green) and Na-K-ATPase (green), 200x.

 

Details

TissueHuman colon (Cn) or rectum (Re) of untreated Ulcerative Colitis.
QCNo bacteria, yeast, fungi, mycoplasma, virus.
BioassayAttach, spread, in Culture Medium.
Cryovial500,000 HCnEpC-UC-N or HReEpC-UC-N in Freezing Medium.
KitCryovial frozen HCnEpC-UC-N (732Cn-UC-N-05a) or HReEpC-UC-N (732Re-UC-N-05a), with Culture Med (716DC-50), Coating Solution (025-05), Thawing Solution (716T-20).
CulturedShipped in flasks or plates in medium.
DoublingsCells can be cultured up to 7 days. Cells do not divide, and cannot be passaged. 
ApplicationsLaboratory research use only (RUO). Not for human, clinical, diagnostic or veterinary use.
Instructions Gastrointestinal EpC

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Products

Product Size CAT.# Price Quantity
Cryopreserved HCnEpC-UC-N, adult: Frozen HCnEpC-UC-N (5x10^5) from left colon, Proctitis, 35 yr old female with untreated Ulcerative Colitis. Size: 1 Cryovial CAT.#: 732Cn-UC-N-05a-0070009 Price: $1,150.00
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Cryopreserved HCnEpC-UC-N, adult: Frozen HCnEpC-UC-N (5x10^5) from left colon, Proctitis, 28 yr old female with untreated Ulcerative Colitis. Size: 1 Cryovial CAT.#: 732Cn-UC-N-05a-0100013 Price: $1,150.00
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Cryopreserved HCnEpC-UC-N, adult: Frozen HCnEpC-UC-N (5x10^5) from right colon, Pancolitis, 46 yr old female with untreated Ulcerative Colitis. Size: 1 Cryovial CAT.#: 732Cn-UC-N-05a-0380203 Price: $1,150.00
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Cryopreserved HReEpC-UC-N, adult: Frozen HReEpC-UC-N (5x10^5) from rectum, Pancolitis 34 yr old male with untreated Ulcerative Colitis. Size: 1 Cryovial CAT.#: 732Re-UC-N-05a-0220088 Price: $1,150.00
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Related Products

Product Size CAT.# Price Quantity
GI Epithelial Cell Defined Culture Medium: All-in-one medium, includes antibiotics. Store at -20C. Once thawed, store at 4C and use within 4 weeks. Size: 50 ml CAT.#: 716DC-50 Price: $55.00
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GI Epithelial Cell Coating Solution: Coating solution for GI Epithelial Cells Size: 5 ml CAT.#: 025-05 Price: $16.00
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GI Epithelial Cell Thawing Solution: Thawing solution for GI Epithelial Cells Size: 20 ml CAT.#: 716T-20 Price: $26.00
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Extended Family Products

Product Size CAT.# Price Quantity
TEER Assay GI Epithelial Cell Medium : All-in-one ready-to-use optimized media for studying GI epithelial barrier function. Store at -20C. Once thawed, store at 4C and use within 4 weeks. Size: 50 ml CAT.#: 716TA-50 Price: $65.00
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Resources/Documents

Cell Apps Flyer Epithelial Cells

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5 Important Cell Culture Rules

Format: PDF

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