Instructions Gastrointestinal EpC
Cell Apps Flyer Epithelial Cells
5 Important Cell Culture Rules

Description

Crohn’s Disease progression can lead to complications such as structuring (B2) and penetrating (B3) behaviors. In addition to Naive/Unreated Crohn’s Disease Gastrointestinal Cells, the Cell Applications IBD epithelial cell biobank covers later stages of Crohn's Disease. Most B2 and B3 patients have been treated with anti-inflammatory steroids and immunosuppressants (anti-TNF medication) to slow progression of disease. Despite medication, many patients continue to struggle, and disease management remains challenging. Only about one-fifth of patients are in remission a year after diagnosis. More than one-third of patients with IBD fail induction therapy, and up to 60 percent of primary responders lose response to medication over time. 

To better understand Crohn’s Disease progression, treatment, and refraction, Cell Applications offers researchers access to a first-of-its-kind Epithelial Cell biobank from Crohn’s Disease. The biobank includes a diverse range of disease age, location, and behavior. Cells originate from colon (Cn), ileum (Il), and Rectum (Re) tissue samples. CD cells are split into Treated and Untreated (naive) Crohn's Disease categories. 

For your convenience, the Epithelial Cell Total Kit includes all the reagents you need for gastrointestinal epithelial cell culture. Along with a cryovial of 500,000 diseased epithelial cells, the Total Kit includes optimized cell culture medium, thawing medium, and coating solution. Cells can be cultured up to 7 days. Cells cannot be passaged and should be plated directly into the experimental system. 

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Crohn's Disease Gastrointestinal Epithelial Cells, Treated (HIlEpC-CD-T): phase contrast 100x, immunostained for ZO-1 (red), Villin (green) and Na-K-ATPase (green), 200x.

Details

TissueHuman colon or ileum of treated Crohn's Disease.
QCNo bacteria, yeast, fungi, mycoplasma, virus.
BioassayAttach, spread, in Culture Medium.
Cryovial500,000 HCnEpC-CD-T or HIlEpC-CD-T in Freezing Medium.
KitCryovial frozen HCnEpC-CD-T (732Cn-CD-T-05a) or HIlnEpC-CD-T (732Il-CD-T-05a), with Culture Med (716DC-50), Coating Solution (025-05), Thawing Solution (716T-20).
CulturedShipped in flasks or plates in medium.
DoublingsCells can be cultured up to 7 days. Cells do not divide, and cannot be passaged. 
ApplicationsLaboratory research use only (RUO). Not for human, clinical, diagnostic or veterinary use.
Instructions Gastrointestinal EpC

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Products

Product Size CAT.# Price Quantity
Cryopreserved HIlEpC-CD-T, adult: Frozen HIlEpC-CD-T (5x10^5) from ileum, ileocolitis, stricturing, 39 yr old male with Crohn's disease treated with Vedolizumab and Adalimumab.   Size: 1 Cryovial CAT.#: 732Il-CD-T-05a-0110014 Price: $1,150.00
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Cryopreserved HIlEpC-CD-T, adult: Frozen HIlEpC-CD-T (5x10^5) from ileum, ileitis, penetrating 26 yr old female with Crohn's disease treated with Infliximab. Size: 1 Cryovial CAT.#: 732Il-CD-T-05a-0420156 Price: $1,150.00
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Related Products

Product Size CAT.# Price Quantity
GI Epithelial Cell Defined Culture Medium: All-in-one medium, includes antibiotics. Store at -20C. Once thawed, store at 4C and use within 4 weeks. Size: 50 ml CAT.#: 716DC-50 Price: $55.00
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GI Epithelial Cell Coating Solution: Coating solution for GI Epithelial Cells Size: 5 ml CAT.#: 025-05 Price: $16.00
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GI Epithelial Cell Thawing Solution: Thawing solution for GI Epithelial Cells Size: 20 ml CAT.#: 716T-20 Price: $26.00
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Extended Family Products

Product Size CAT.# Price Quantity
TEER Assay GI Epithelial Cell Medium : All-in-one ready-to-use optimized media for studying GI epithelial barrier function. Store at -20C. Once thawed, store at 4C and use within 4 weeks. Size: 50 ml CAT.#: 716TA-50 Price: $65.00
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Resources/Documents

Cell Apps Flyer Epithelial Cells

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5 Important Cell Culture Rules

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