Instructions RSpN
5 Important Cell Culture Rules
MSDS Cryopreserved Cells
Cell Apps Flyer Nervous System
Cell Apps Flyer Brain Cells
Cell Apps Poster Primary Cells
Cell Applications Inc Brochure
Description
Rat Spinal Cord Neurons (RSpN) are derived from spinal cords of normal embryonic rat (gestation day 15) by standardized methods. When cultured under the recommended conditions, RSpN arborize and form complex neurite network in one week. RSpN Stain positive for β III-Tubulin.
The spinal cord is consisted of tubular bundles of nervous tissues and support cells. Extending from the medulla oblongata of the brainstem, the spinal cord transmits neural signals between the brain and the rest of the body, and plays a key role in somatosensory and motor functions. Spinal cord injuries, diseases and degeneration, such as Friedreich's ataxia, Amyotrophic lateral sclerosis, and spinal muscular atrophies, cause weakening or loss of sensory and motor functions. Studies using spinal cord neurons will allow a better understanding of the disease mechanisms, physiopathologies, and advancement in drug development and therapies. Cultures of spinal cord neurons can be applied for a variety of experiments including toxicity test, immunostaining, live cell imaging, co-culturing, electrophysiology, and more.
(Click to Enlarge) Cryopreserved Rat Spinal Cord Neurons (RSpN) revived and cultured for 6 days (top), stained (bottom) on day 7 for β III-Tubulin (green), GFAP (red), and DAPI (blue).
Details
| Tissue | Normal healthy rat spinal cord |
| QC | No bacteria, yeast, fungi, mycoplasma, virus |
| Character | Positive for β-III Tubulin |
| Bioassay | Plate on Poly-D-Lysine coated surface, Arborize to form neurite network in Culture Med |
| Cryovial | 2 or 4M RSpN in Ser-Fr Frzng Med (042-50) |
| Doublings | N/A - Neurons don't proliferate in vitro |
| Applications | Laboratory research use only (RUO). Not for human, clinical, diagnostic or veterinary use. |
Products
| Product | Size | CAT.# | Price | Quantity |
|---|---|---|---|---|
| Cryopreserved Rat Spinal Neurons (RSpN): Frozen RSpN (2x10^6) | Size: 1 Cryovial | CAT.#: R8814N-20 | Price: $707.00 |
Related Products
| Product | Size | CAT.# | Price | Quantity |
|---|---|---|---|---|
| Rat Neuron Culture Medium: For the culture and maintenance of neurons. (For rat dorsal root gangion neurons, use Cat# R823K-100.) | Size: 100 ml | CAT.#: R817-100 | Price: $72.00 | |
| Rat Neuron Culture Medium Kit: Basal medium & growth supplement sold together packaged separately. (For rat dorsal root gangion neurons, use Cat# R823K-100.) | Size: Yields 100 ml | CAT.#: R817K-100 | Price: $83.00 | |
| Rat Neuron Basal Medium: Basal medium (contains no growth supplement). Add GS before use. | Size: 100 ml | CAT.#: R816-100 | Price: $40.00 | |
| Rat Neuron Culture Supplement: Added to Basal Medium to create Culture Medium | Size: 3 ml | CAT.#: R817-GS | Price: $57.00 | |
| Neuron Coating Solution I: Poly-D-Lysine coating for neurons | Size: 5 ml | CAT.#: 027-05 | Price: $18.00 | |
| Neuron Coating Solution I: Poly-D-Lysine coating for neurons | Size: 10 ml | CAT.#: 027-10 | Price: $36.00 | |
| Rat Neuron Plating Solution I: For initial plating of neurons | Size: 10 ml | CAT.#: R817P-10 | Price: $23.00 | |
| Rat Neuron Plating Solution I: For initial plating of neurons | Size: 20 ml | CAT.#: R817P-20 | Price: $45.00 | |
| Rat Neuron Plating Solution I: For initial plating of neurons | Size: 40 ml | CAT.#: R817P-40 | Price: $71.00 |
Extended Family Products
| Product | Size | CAT.# | Price | Quantity |
|---|---|---|---|---|
| Cytofect Neuron Transfection Kit (100 x 24-Wells): 100 x 24-Well Rxns | Size: 1 Kit | CAT.#: TF886K | Price: $465.00 | |
| Cytofect Neuron Transfection Sample Kit (25 x 24-Wells): 25 x 24-Well Rxns | Size: 1 Sample Kit | CAT.#: TF886KS | Price: $62.00 | |
| Cyto-X Cell Counting Reagent: 500 tests | Size: 1 Bottle | CAT.#: 028-01 | Price: $160.00 | |
| Cyto-X Cell Counting Reagent Sample: 100 tests | Size: Sample | CAT.#: 028-S | Price: $41.00 |