Product Sheet CG1654
Description
BACKGROUND Alpha-Catenin functions as a linking protein between cadherins and actin-containing filaments of the cytoskeleton. It has been reported that the actin binding proteins vinculin and alpha-actinin can bind to alpha-Catenin. However, a protein complex including a cadherin, actin, beta-Catenin and alpha-Catenin has not been isolated. It has been suggested that alpha-Catenin does not bind with high affinity to both actin filaments and the E-Cadherin-beta-Catenin complex at the same time.1 It has been observed that when alpha-Catenin is not in a molecular complex with beta-Catenin, it dimerizes and functions to regulate actin filament assembly, possibly by competing with Arp2/3 protein.2 The amino acid sequence of alpha-Catenin has sequence similarity to that of vinculin. There are three human alpha-Catenin genes: alpha-1-Catenin (also called alpha-E-Catenin), alpha-2-Catenin (also called alpha-N-Catenin), and alpha-3-Catenin (also called alpha-T-Catenin).
REFERENCES
1. Yamada, S. et al: Cell 123:889-901, 2005
2. Drees, S. et al: Cell 123:903-915, 2005
2. Drees, S. et al: Cell 123:903-915, 2005
Products are for research use only. They are not intended for human, animal, or diagnostic applications.
(Click to Enlarge)
Top: Immunoblotting analysis of extracts from HeLa cells, using Anti-Catenin-α1, C-Terminal antibody. The lane on the left was treated with the Anti-Catenin-α1, C-Terminal antibody. The lane on the right (negative control) was treated with both Anti-Catenin-α1, C-Terminal antibody and the synthesized immunogen peptide. Bottom: Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using Anti-Catenin-α1, C-Terminal antibody. Cells on the left were treated with the Anti-Catenin-α1, C-Terminal antibody. Cells on the right (negative control) were treated with both Anti-Catenin-α1, C-Terminal antibody and the synthesized immunogen peptide.
Details
Cat.No.: | CG1654 |
Antigen: | Synthesized peptide derived from human Catenin-α1. |
Isotype: | Rabbit IgG |
Species & predicted species cross- reactivity ( ): | Human, Rat, Mouse |
Applications & Suggested starting dilutions:* | WB 1:500-1:1000 IP n/d IHC 1:50-1:100 ICC n/d FACS n/d |
Predicted Molecular Weight of protein: | 100 kDa |
Specificity/Sensitivity: | Detects endogenous α1-Catenin without cross-reactivity with other family members. |
Storage: | Store at -20°C, 4°C for frequent use. Avoid repeated freeze-thaw cycles. |
*Optimal working dilutions must be determined by end user.
Products
| Product | Size | CAT.# | Price | Quantity |
|---|---|---|---|---|
| Rabbit Alpha1 Catenin Antibody: Rabbit alpha1 Catenin Antibody | Size: 100 ul | CAT.#: CG1654 | Price: $384.00 |