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Rat Neural Stem Cells: RNSC

Top: Rat Neural Stem Cells, RNSC (neurospheres). Bottom: Differentiated RNSC immunofluorescently labeled for bIII-tubulin(green, neuronal marker) and GFAP (red, astrocyte marker).

Rat Neural Stem Cells (RNSC) are maintained in undifferentiated proliferative state by culturing them as free floating neurospheres in serum-free medium optimized with growth factors. They are cryopreserved at first passage and can be cultured and propagated for 1-2 passages prior to induction of phenotypic differentiation. These stem cells are multipotent and can be induced to differentiate into neurons, astrocytes and oligodendrocytes that make up the central nervous system (CNS).  The ability of neural stem cells to retain multi-lineage potential and proliferate extensively in vitro provides new avenues for the treatment of neural degenerative diseases and injuries. 

Characterization: differentiated neural stem cells are positive for β-tubulin III, GFAP and oligodendrocyte marker O4.

Tissue:
E18 fetal or adult rat brain cerebral striatum and mesencephalon.  Each lot is tested negative for mycoplasma, bacteria, and fungi.
Cryopreserved ampoule:
1st passage, >2 million cells in Rat Neural Stem Cell Basal Medium that contains 10% FBS and 10% DMSO.
Kit contains:
Ampoule of cryopreserved RNSC (RS820-20f), 250 ml of Rat Neural Stem Cell Growth Medium (R813-250).
Proliferating Cells:
 
Shipped in Rat Neural Stem Cell Growth Medium at 2nd passage in either flasks or multiwell dishes.
Population doublings:
Can be cultured 1-2 passages

Products

Cryopreserved RNSC, fetal

Pricing
Description
Size
Cat. #
Price
Cryopreserved RNSC, fetal Each kit contains an ampoule of >2 million cryopreserved fetal RNSC, 250 ml of Rat Neural Stem Cell Growth Medium (R813-250), and two 10 cm non-tissue culture treated dishes. 1 Kit RS820K-05f $665.00
Cryopreserved RNSC, fetal 1 Ampoule RS820-20f $565.00

Proliferating RNSC, fetal

Pricing
Description
Size
Cat. #
Price
Proliferating RNSC, fetal Cultured and shipped at second passage in Rat Neural Stem Cell Growth Medium. T-25 Flask RS821-25f $565.00
Proliferating RNSC, fetal T-75 Flask RS821-75f $755.00
Proliferating RNSC, fetal 6 well RS821-6Wf $755.00
Proliferating RNSC, fetal 96 well RS821-96Wf $875.00

Related Products

Rat Neural Stem Cell Basal Medium

Pricing
Description
Size
Cat. #
Price
Rat Neural Stem Cell Basal Medium Growth Medium without Growth Supplement 250 ml R812-250 $37.00

Rat Neural Stem Cell Differentiation Medium

Pricing
Description
Size
Cat. #
Price
Rat Neural Stem Cell Differentiation Medium Rat Neural Stem Cell Differentiation Medium 250 ml R813D-250 $100.00

Rat Neural Stem Cell Growth Medium

Pricing
Description
Size
Cat. #
Price
Rat Neural Stem Cell Growth Medium All-in-one ready-to-use media 250 ml R813-250 $110.00

Rat Neural Stem Cell Growth Medium Kit

Pricing
Description
Size
Cat. #
Price
Rat Neural Stem Cell Growth Medium Kit Basal Medium and Growth Supplement sold together, bottled separately yields 250 ml R813K-250 $119.00

Rat Neural Stem Cell Growth Supplements

Pricing
Description
Size
Cat. #
Price
Rat Neural Stem Cell Growth Supplements Added to Basal Medium to create Growth Medium 10 ml R813-GS $82.00

Subculture Reagent Kit

Pricing
Description
Size
Cat. #
Price
Subculture Reagent Kit 100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution 100 ml 090K $51.00