MSDS Cryopreserved Cells
5 Important Cell Culture Rules
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Instructions HCK

Description

Human Corneal Keratocytes (HCK) are primary cells derived from human eye. Corneal Keratocytes are neural-crest derived mesenchymal cells that populate the corneal stroma. The stroma is comprised of a type I and type V collagen fibers arranged as lamellae, makes up majority of the corneal thickness responsible for promoting corneal transparency. Upon corneal injury, keratocytes are stimulated to either undergo apoptosis or transition into repair phenotypes to promote corneal regeneration.
 
Human Corneal Keratocytes (HCK) cultured in Corneal Keratocyte Growth Medium are able to propagate to 10 population doublings when cultured under the recommended conditions. HCK could be used as an important in vitro cell model to study keratocyte differentiation, corneal injury and related diseases.
 
Human Corneal Keratocytes: HCK Top: Phase-contrast, Bottom Left: HCK immunostained for Vimentin (green), Bottom Right: Fibronectin (green), nuclei are stained with DAPI.

Details

TissueNormal healthy human cornea
QCNo bacteria, yeast, fungi, mycoplasma, virus
BioassayAttach, spread, proliferate in Growth Med
Cryovial500,000 HCK (1st passage) frozen in Basal Medium w/ 10% FBS, 10% DMSO
KitCryovial frozen HCK (632-05a), Growth Medium (6111-500), Subculture Rgnt Kit (090K)
ProliferatingShipped in Tsfr Med, psg 1, flasks or plates
DoublingsAt least 15
ApplicationsLaboratory research use only (RUO). Not for human, clinical, diagnostic or veterinary use.

 
Instructions HCK

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MSDS Cryopreserved Cells

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Products

Product Size CAT.# Price Quantity
Cryopreserved Human Corneal Keratocytes Cells Total Kit, adult: 5x10^5 Cells (Adult), Medium & Subculture Reagents (See Details tab for specifics) Size: 1 Kit CAT.#: 632K-05a Price: $878.00
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Cryopreserved Corneal Keratocytes Cells (HCK), adult: Frozen HCK (5x10^5) Size: 1 Cryovial CAT.#: 632-05a Price: $715.00
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Proliferating Corneal Keratocytes Cells (HCK), adult: Actively growing, dividing cells in medium Size: T-25 Flask CAT.#: 633-25a Price: $715.00
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Proliferating Corneal Keratocytes Cells (HCK), adult: Actively growing, dividing cells in medium Size: T-75 Flask CAT.#: 633-75a Price: $905.00
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Proliferating Corneal Keratocytes Cells (HCK), adult: Actively growing, dividing cells in medium Size: 24 Well CAT.#: 633-24Wa Price: $905.00
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Proliferating Corneal Keratocytes Cells (HCK), adult: Actively growing, dividing cells in medium Size: 96 Well CAT.#: 633-96Wa Price: $1,025.00
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Related Products

Product Size CAT.# Price Quantity
Corneal Keratocyte Growth Medium: All-in-one ready-to-use Size: 500 ml CAT.#: 6111-500 Price: $110.00
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Corneal Keratocyte Growth Medium Kit: Basal medium & growth supplement sold together packaged separately Size: 1 Kit CAT.#: 6111K-500 Price: $121.00
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Corneal Keratocyte Basal Medium : Basal medium (contains no growth supplement). Add GS before use. Size: 500 ml CAT.#: 6110-500 Price: $72.00
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Corneal Keratocyte Growth Supplement: Added to Basal Medium to create Growth Medium Size: 50 ml CAT.#: 6111-GS Price: $65.00
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Extended Family Products

Product Size CAT.# Price Quantity
Cyto-X Cell Counting Reagent: 500 tests Size: 1 Bottle CAT.#: 028-01 Price: $160.00
Cyto-X Cell Counting Reagent Sample: 100 tests Size: Sample CAT.#: 028-S Price: $41.00
Subculture Reagent Kit: 100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution Size: 1 Kit CAT.#: 090K Price: $63.00
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Freezing Medium: For general cryopreservation of most primary cells. Contains FBS & DMSO. Size: 50 ml CAT.#: 040-50 Price: $54.00
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Resources/Documents

5 Important Cell Culture Rules

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Cell Apps Poster Primary Cells

Format: PDF

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Cell Applications Inc Brochure

Format: PDF

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