5 Important Cell Culture Rules
MSDS Cryopreserved Cells
Cell Apps Flyer Nervous System
Cell Apps Flyer Brain Cells
Cell Apps Poster Primary Cells
Brochure Cell Applications Inc
Instructions MCoN

Description

MCoN (Mouse Cortical Neurons) are cryopreserved primary mouse neurons derived from the cerebral cortex of day 18 embryonic CD1 mouse brains. When cultured under the recommended conditions, MCoN arborize and form complex neurite network in one week. MCoN Stain positive for β III-Tubulin. 
  
The cerebral cortex plays a key role in cognition, memory, attention, perceptual awareness, thought, language and consciousness. Neurons connect to other neural cells through neurites and synapses to form complex neurocircuitry for signal transmission. A healthy circuitry in the brain is essential for cognitive functions, proper control and regulation of the other parts of the body. Dysfunction and degeneration in the cerebral cortex have been implicated in various brain injuries, neurological and psychiatric disorders. Cortical neurons, therefore, provide an excellent cellular model system to study disease mechanisms and pathophysiologies.  They also serve as a platform for drug screening and development, toxicity tests, immunostaining, live cell imaging, co-culturing and electrophysiology.
(Click to Enlarge) Freshly isolated Mouse Cortical Neurons (MCoN) cultured for 7 days. A: Phase contrast, 100x. B: Immunostaining for β-III Tubulin (green), GFAP (red), DAPI (blue), 100x.

Details

TissueNormal healthy mouse brain  
QCNo bacteria, yeast, fungi, mycoplasma
CharacterPositive for β-III Tubulin
BioassayPlate on Poly-D-Lysine coated surface, arborize to form neurite network in Culture Med
Cryovial2M MCoN cryopreserved after isolation in Serum-Free Freezing Medium (042-50)
Kit
2M cryopreserved MCoN, Coating Soln I (027-05), Plating Med (M817P-10), Culture Med (M817-100)
 Doublings N/A - Neurons don't proliferate in vitro
 ApplicationsLaboratory research use only (RUO). Not for human, clinical, diagnostic or veterinary use.
Instructions MCoN

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MSDS Cryopreserved Cells

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Products

Product Size CAT.# Price Quantity
Cryopreserved Mouse Cortical Neurons Total Kit: 2x10^6 Cells, Plating Medium, Culture Medium & Coating Solution (See Details tab for specifics) Size: 1 Kit CAT.#: M882NK-20 Price: $685.00
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Cryopreserved Mouse Cortical Neurons (MCoN): Frozen MCoN (2x10^6). Size: 1 Cryovial CAT.#: M882N-20 Price: $555.00
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Cryopreserved MCoN Total Kit: 1x10^6 Cells, Plating Medium, Culture Medium & Coating Solution (See Details tab for specifics) Size: 1 Kit CAT.#: M882NK-10 Price: $540.00
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Cryopreserved MCoN: Frozen MCoN (1x10^6) Size: 1 Cryovial CAT.#: M882N-10 Price: $418.00
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Related Products

Product Size CAT.# Price Quantity
Mouse Neuron Cell Growth Medium, 100ml: All-in-one ready-to-use media Size: 100 ml CAT.#: M817-100 Price: $90.00
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Mouse Neuron Culture Medium Kit, 100ml: Basal Medium and Growth Supplement sold together, bottled separatedly Size: Yields 100 ml CAT.#: M817K-100 Price: $99.00
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Mouse Neuron Basal Medium: Basal medium (contains no growth supplement).  Add GS before use. Size: 100 ml CAT.#: M816-100 Price: $40.00
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Mouse Neuron Culture Supplement: Added to Basal Medium to create Culture Medium Size: 3 ml CAT.#: M817-GS Price: $64.00
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Mouse Neuron Plating Solution, 20ml: For initial plating of neurons Size: 20 ml CAT.#: M817P-20 Price: $44.00
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Mouse Neuron Plating Solution, 10ml: For initial plating of neurons Size: 10 ml CAT.#: M817P-10 Price: $23.00
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Neuron Coating Solution I: Poly-D-Lysine coating for neurons Size: 10 ml CAT.#: 027-10 Price: $36.00
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Neuron Coating Solution I: Poly-D-Lysine coating for neurons Size: 5 ml CAT.#: 027-05 Price: $18.00
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Extended Family Products

Product Size CAT.# Price Quantity
Cytofect Neuron Transfection Kit (100 x 24-Wells): 100 x 24-Well Rxns Size: 1 Kit CAT.#: TF886K Price: $465.00
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Cytofect Neuron Transfection Sample Kit (25 x 24-Wells): 25 x 24-Well Rxns Size: 1 Sample Kit CAT.#: TF886KS Price: $62.00
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Cyto-X Cell Counting Reagent: 500 tests Size: 1 Bottle CAT.#: 028-01 Price: $160.00
Cyto-X Cell Counting Reagent Sample: 100 tests Size: Sample CAT.#: 028-S Price: $41.00

Resources/Documents

5 Important Cell Culture Rules

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Cell Apps Flyer Nervous System

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Cell Apps Flyer Brain Cells

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Cell Apps Poster Primary Cells

Format: PDF

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