Description

Total RNA prepared from normal human tissues using the modified guanidium thiocyanate method.  RNA is DNAse treated to remove contaminating genomic DNA and undergoes stringent quality control tests.  Purified RNA is ready for use in convenient 50 & 250 µg sizes for various downstream applications, including microarray hybridization, PCR cDNA synthesis and RT-PCR analysis.
 
See also Cell RNA

(Click to Enlarge) Tissue RNA.  Human Fetal Brain (lines 2, 3) and Human Adult Lung (lines 4, 5) Total RNA.  RNA was electrophoresed in 1% agarose gel to show the distinct 28S and 18S ribosomal RNA bands (lines 2, 4).  RT-PCR was performed with HSP60 primers to produce 2.2 kb product (lines 3, 5).

Details

Stringent quality control tests confirm RNA integrity and stability:

  • Distinct 28S and 18S ribosomal RNA were shown on 1% denaturing gel before and after 37C  incubation
  • No visible Genomic DNA band of 23 kb detected
  • No genomic DNA contamination detected following PCR amplification using house-keeping gene G3PDH primers
  • A 2.2 kb product from mRNA was detected following RT-PCR amplification using human heat shock gene Hsp60 primers
  • A discrete band of 2 kb was detected upon hybridization with a human beta-actin cDNA probe in Northern Blot analysis
  • Primers were designed to detect mRNA for genes of interest to confirm cell phenotype

Products

Product Size CAT.# Price Quantity
Human Brain RNA: Total RNA prepared from human brain tissue, fetal Size: 50 ug CAT.#: 1F01-50 Price: $138.57
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Human Brain RNA: Total RNA prepared from human brain tissue, fetal Size: 250 ug CAT.#: 1F01-250 Price: $521.98
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Human Heart RNA: Total RNA prepared from human heart tissue Size: 50 ug CAT.#: 1H30-50 Price: $138.57
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Human Heart RNA: Total RNA prepared from human heart tissue Size: 250 ug CAT.#: 1H30-250 Price: $521.98
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Human Intestine RNA: Total RNA prepared from human small intestine tissue Size: 50 ug CAT.#: 1H24-50 Price: $138.57
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Human Intestine RNA: Total RNA prepared from human small intestine tissue Size: 250 ug CAT.#: 1H24-250 Price: $521.98
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Human Kidney RNA: Total RNA prepared from human kidney tissue Size: 50 ug CAT.#: 1H50-50 Price: $138.57
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Human Kidney RNA: Total RNA prepared from human kidney tissue Size: 250 ug CAT.#: 1H50-250 Price: $521.98
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Human Liver RNA: Total RNA prepared from human liver tissue, fetal Size: 50 ug CAT.#: 1F21-50 Price: $138.57
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Human Liver RNA: Total RNA prepared from human liver tissue, fetal Size: 250 ug CAT.#: 1F21-250 Price: $521.98
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Human Liver RNA: Total RNA prepared from human liver tissue Size: 50 ug CAT.#: 1H21-50 Price: $138.57
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Human Liver RNA: Total RNA prepared from human liver tissue Size: 250 ug CAT.#: 1H21-250 Price: $521.98
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Human Lung RNA: Total RNA prepared from human lung tissue Size: 50 ug CAT.#: 1H40-50 Price: $138.57
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Human Lung RNA: Total RNA prepared from human lung tissue Size: 250 ug CAT.#: 1H40-250 Price: $521.98
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Human Pancreas RNA: Total RNA prepared from human pancreas tissue Size: 50 ug CAT.#: 1H23-50 Price: $138.57
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Human Pancreas RNA: Total RNA prepared from human pancreas tissue Size: 250 ug CAT.#: 1H23-250 Price: $521.98
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Human Placental RNA: Total RNA prepared from human placental tissue Size: 50 ug CAT.#: 1H54-50 Price: $138.57
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Human Placental RNA: Total RNA prepared from human placental tissue Size: 250 ug CAT.#: 1H54-250 Price: $521.98
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Human Prostate RNA: Total RNA prepared from human prostate tissue Size: 50 ug CAT.#: 1H52-50 Price: $138.57
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Human Prostate RNA: Total RNA prepared from human prostate tissue Size: 250 ug CAT.#: 1H52-250 Price: $521.98
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Human Skeletal Muscle RNA: Total RNA prepared from human skeletal muscle tissue Size: 50 ug CAT.#: 1H60-50 Price: $138.57
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Human Skeletal Muscle RNA: Total RNA prepared from human skeletal muscle tissue Size: 250 ug CAT.#: 1H60-250 Price: $521.98
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Human Spleen RNA: Total RNA prepared from human spleen tissue Size: 250 ug CAT.#: 1H70-250 Price: $521.98
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Human Spleen RNA: Total RNA prepared from human spleen tissue Size: 50 ug CAT.#: 1H70-50 Price: $138.57
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Human Stomach RNA: Total RNA prepared from human stomach tissue Size: 50 ug CAT.#: 1H20-50 Price: $138.57
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Human Stomach RNA: Total RNA prepared from human stomach tissue Size: 250 ug CAT.#: 1H20-250 Price: $521.98
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Human Testes RNA: Total RNA prepared from human testes tissue Size: 50 ug CAT.#: 1H51-50 Price: $138.57
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Human Testes RNA: Total RNA prepared from human testes tissue Size: 250 ug CAT.#: 1H51-250 Price: $521.98
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Related Products

Product Size CAT.# Price Quantity
Custom RNA Services: Total RNA from Human Tissues Size: 1 Project CAT.#: CSTSVC-RHT Price: $0.00
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