Instructions RPF
5 Important Cell Culture Rules
MSDS Cryopreserved Cells
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Description

Rat Perineurial Fibroblasts (RPF) from Cell Applications, Inc. are of mesenchymal origin and form the perineurium, one of the three connective tissue compartments of the peripheral nerve. The perineurium wraps around nerve fascicles to form a metabolically active diffusion barrier. It provides protection to the nerve bundles, maintains the homeostasis of the endoneurium, modulates external stretching forces that exerts to the nerve, and regulates endoneurial pressures. 
RPF and other cells of mesenchymal origin express the cytoplasmic protein Vimentin, a Type III intermediate filament that forms part of the cytoskeleton.  By helping to maintain cell shape, cytoplasmic integrity and cytoskeletal interactions, Vimentin functions in cell adhesion, migration and signaling.
Perineurial fibroblasts also express fibronectin, a multifunctional extracellular matrix glycoprotein.  Like vimentin, fibronectin plays major roles in cell morphology, cytoskeletal organization, adhesion, growth and migration. Fibronectin, which binds other ECM molecules, also functions in cell differentiation, embryonic development, hemostasis, phagocytosis, wound healing and repair.
(Click to Enlarge) Rat Perineurial Fibroblasts (RPF) revived (top) and stained for Vimentin (green, bottom left), Fibronectin (green, bottom right), and DAPI (blue).

Details

TissueNormal healthy rat peripheral nerves
QCNo bacteria, yeast, fungi, mycoplasma
CharacterPositive for Fibronectin
BioassayAttach, spread, proliferate in Growth Med
Cryovial500,000 RPF (1st passage) frozen in Basal Medium w/ 10% FBS, 10% DMSO
KitCryovial frozen RPF (R852-05n), Growth Medium (R827-500), Subcltr Rgnt Kit (090K)
ProliferatingShipped in Gr Med, 1st psg (flasks or plates)
DoublingsAt least 12
ApplicationsLaboratory research use only (RUO). Not for human, clinical, diagnostic or veterinary use.
Instructions RPF

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Products

Product Size CAT.# Price Quantity
Cryopreserved RPF Total Kit, Neonatal: 5x10^5 Cells (Neonatal), Medium & Subculture Reagents (See Details tab for specifics) Size: 1 Kit CAT.#: R852K-05n Price: $430.50
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Cryopreserved RPF, Neonatal: Frozen RPF (5x10^5) Size: 1 Cryovial CAT.#: R852-05n Price: $358.75
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Proliferating RPF, Neonatal: Actively growing, dividing cells in medium Size: T-25 Flask CAT.#: R853-25n Price: $358.75
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Proliferating RPF, Neonatal: Actively growing, dividing cells in medium Size: 6 Well CAT.#: R853-6Wn Price: $466.38
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Proliferating RPF, Neonatal: Actively growing, dividing cells in medium Size: T-75 Flask CAT.#: R853-75n Price: $466.38
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Proliferating RPF, Neonatal: Actively growing, dividing cells in medium Size: 96 Well CAT.#: R853-96Wn Price: $538.13
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Related Products

Product Size CAT.# Price Quantity
Rat Perineurial Fibroblast Basal Medium: Basal medium (contains no growth supplement). Add GS before use. Size: 500 ml CAT.#: R826-500 Price: $57.40
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Rat Perineurial Fibroblast Growth Medium: All-in-one ready-to-use media Size: 500 ml CAT.#: R827-500 Price: $103.53
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Rat Perineurial Fibroblast Growth Medium Kit: Basal medium & growth supplement sold together packaged separately Size: Yields 500 ml CAT.#: R827K-500 Price: $109.68
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Rat Perineurial Fibroblast Growth Supplement: Added to Basal Medium to create Growth Medium Size: 25 ml CAT.#: R827-GS Price: $56.38
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Extended Family Products

Product Size CAT.# Price Quantity
Anti-Vimentin: Mouse Vimentin Antibody Size: 100 ul CAT.#: CP10244 Price: $333.13
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Cell Freezing Medium: For general cryopreservation of most primary cells. Contains FBS & DMSO. Size: 50 ml CAT.#: 040-50 Price: $53.81
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Cyto-X Colorimetric Cell Counting Reagent: 500 tests Size: 1 Bottle CAT.#: 028-01 Price: $138.84
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Cyto-X Colorimetric Cell Counting Reagent: 100 tests Size: Sample CAT.#: 028-S Price: $35.88
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Cytofect™ Fibroblast Transfection Kit: 250 x 24-Well Rxns Size: 1 Kit CAT.#: TF103K Price: $403.59
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Cytofect™ Fibroblast Transfection Kit: 25 x 24-Well Rxns Size: 1 Sample Kit CAT.#: TF103KS Price: $53.76
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Fibronectin ELISA Kit, Rat: Rat Fibronectin ELISA Kit Size: 96 Wells CAT.#: CL0350 Price: $500.46
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Subculture Reagent Kit: 100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution Size: 1 Kit CAT.#: 090K Price: $54.89
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Resources/Documents

5 Important Cell Culture Rules

Format: PDF

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Cell Apps Flyer Nervous System

Format: PDF

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Cell Apps Poster Primary Cells

Format: PDF

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Cell Applications Inc Brochure

Format: PDF

Downoad Now