Instructions RHiN
5 Important Cell Culture Rules
MSDS Cryopreserved Cells
Cell Apps Flyer Nervous System
Cell Apps Flyer Brain Cells
Cell Apps Poster Primary Cells
Cell Applications Inc Brochure

Description

Rat Hippocampal Neurons (RHiN) are derived from the hippocampi of normal embryonic rats by standardized methods. When cultured under the recommended conditions, RHiN arborize and form complex neurite network in one week. RHiN Stain positive for β III-Tubulin.  RHiN are available in multiple formats, including cryopreserved neurons, freshly isolated primary neurons in suspension, or pre-plated cultured neurons.
The hippocampus, a major brain component located under the cerebral cortex, consists of two hippocampi, one in each side of the brain.  Belonging to the limbic system, the hippocampus functions in spacial memory and navigation, and the consolidation of information from short- to long-term memory. Hippocampal neurons provide a model in vitro system for studying neurophysiology, neural plasticity and memory storage.  Damage, disease or oxygen starvation in this region is associated with Alzheimer's disease, encephalitis, epilepsy and amnesia. Examining hippocampal neurons in those disease conditions will allow a better understanding of brain physiology, disease mechanisms, pathology, and foster advancements in drug development and ultimate clinical applications.
(Click to Enlarge) Freshly isolated Rat Hippocampal Neurons (RHiN) cultured for 5 days (A). Freshly isolated RHiN (B), and revived cryopreserved RHiN (C) stained on day 8 for β III-Tubulin (green), GFAP (red), and DAPI (blue).

Details

TissueNormal healthy rat hippocampus 
QCNo bacteria, yeast, fungi, mycoplasma
CharacterPositive for β-III Tubulin
BioassayPlate on Poly-D-Lysine coated surface, arborize to form neurite network in Culture Med
Cryovial
1M RHiN cryopreserved after isolation in Serum-Free Freezing Med (042-50)
Fresh Culture
Contact CAI if interested in freshly isolated RHiN (suspension or plated)
Plated
RHiN from cyrovial, shipped in Culture Medium
Cryo Kit
RHiN (R886N-10), Cltr Med (R817-100) Ctng Soln I (027-05), Pltng Med (R886P-10)
Suspn Kit
Frsh RHiN (R887N-10), Cultr Med (R817-100) Ctng Soln I (027-05), Pltng Med (R886P-10)
Plated Kit
RHiN in multiwell plates, Cultr Med (R817-50)
DoublingsN/A - Neurons don't proliferate in vitro
ApplicationsLaboratory research use only (RUO). Not for human, clinical, diagnostic or veterinary use.
Instructions RHiN

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MSDS Cryopreserved Cells

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Products

Product Size CAT.# Price Quantity
Cryopreserved RHiN: Frozen RHiN (1x10^6) Size: 1 Cryovial CAT.#: R886N-10 Price: $408.98
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Cryopreserved RHiN Total Kit: 1x10^6 Cells, Plating Medium, Culture Medium & Coating Solution (See Details tab for specifics) Size: 1 Kit CAT.#: R886NK-10 Price: $540.71
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Freshly isolated RHiN: Contact CAI for details Size: 1 Tube CAT.#: R887NF-10 Price: $408.98
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Pre-plated freshly isolated RHiN: Contact CAI for details Size: 6 Well CAT.#: R887NF-6W Price: $0.00
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Pre-plated freshly isolated RHiN: Contact CAI for details Size: 24 Well CAT.#: R887NF-24W Price: $0.00
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Pre-plated freshly isolated RHiN: Contact CAI for details Size: 96 Well CAT.#: R887NF-96W Price: $0.00
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Pre-plated from cryopreserved RHiN: Plated RHiN from cryopreserved vials Size: 6 Well CAT.#: R887N-6W Price: $538.13
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Pre-plated from cryopreserved RHiN: Plated RHiN from cryopreserved vials Size: 24 Well CAT.#: R887N-24W Price: $563.75
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Pre-plated from cryopreserved RHiN: Plated RHiN from cryopreserved vials Size: 96 Well CAT.#: R887N-96W Price: $604.75
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Related Products

Product Size CAT.# Price Quantity
Neuron Coating Solution I: Poly-D-Lysine coating for neurons Size: 10 ml CAT.#: 027-10 Price: $30.75
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Neuron Coating Solution I: Poly-D-Lysine coating for neurons Size: 5 ml CAT.#: 027-05 Price: $16.14
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Rat Hippocampal Neuron Plating Medium: Use for initial plating of RHiN. Size: 10 ml CAT.#: R886P-10 Price: $20.45
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Rat Neuron Basal Medium: Basal medium (contains no growth supplement).  Add GS before use. Size: 100 ml CAT.#: R816-100 Price: $31.21
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Rat Neuron Culture Medium : For the culture and maintenance of neurons. Size: 100 ml CAT.#: R817-100 Price: $63.50
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Rat Neuron Culture Medium Kit: Basal medium & growth supplement sold together packaged separately Size: Yields 100 ml CAT.#: R817K-100 Price: $72.11
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Rat Neuron Culture Supplement: Added to Basal Medium to create Culture Medium Size: 3 ml CAT.#: R817-GS Price: $57.04
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Extended Family Products

Product Size CAT.# Price Quantity
Cyto-X Colorimetric Cell Counting Reagent: 500 tests Size: 1 Bottle CAT.#: 028-01 Price: $138.84
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Cyto-X Colorimetric Cell Counting Reagent: 100 tests Size: Sample CAT.#: 028-S Price: $35.88
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Cytofect™ Neuron Transfection Kit: 100 x 24-Well Rxns Size: 1 Kit CAT.#: TF886K Price: $403.59
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Cytofect™ Neuron Transfection Kit: 25 x 24-Well Rxns Size: 1 Sample Kit CAT.#: TF886KS Price: $53.76
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Rat Neuron Culture Medium : For the culture and maintenance of neurons Size: 150 ml CAT.#: R817-150 Price: $92.25
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Resources/Documents

5 Important Cell Culture Rules

Format: PDF

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Cell Apps Flyer Nervous System

Format: PDF

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Cell Apps Flyer Brain Cells

Format: PDF

Downoad Now
Cell Apps Poster Primary Cells

Format: PDF

Downoad Now
Cell Applications Inc Brochure

Format: PDF

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Publications

2017

(Publications)