Instructions RHiN
5 Important Cell Culture Rules
MSDS Cryopreserved Cells
Cell Apps Flyer Nervous System
Cell Apps Flyer Brain Cells
Cell Apps Poster Primary Cells
Cell Applications Inc Brochure
Description
Rat Hippocampal Neurons (RHiN) are derived from the hippocampi of normal embryonic rats by standardized methods. When cultured under the recommended conditions, RHiN arborize and form complex neurite network in one week. RHiN Stain positive for β III-Tubulin. RHiN are available in multiple formats, including cryopreserved neurons, freshly isolated primary neurons in suspension, or pre-plated cultured neurons.
The hippocampus, a major brain component located under the cerebral cortex, consists of two hippocampi, one in each side of the brain. Belonging to the limbic system, the hippocampus functions in spacial memory and navigation, and the consolidation of information from short- to long-term memory. Hippocampal neurons provide a model in vitro system for studying neurophysiology, neural plasticity and memory storage. Damage, disease or oxygen starvation in this region is associated with Alzheimer's disease, encephalitis, epilepsy and amnesia. Examining hippocampal neurons in those disease conditions will allow a better understanding of brain physiology, disease mechanisms, pathology, and foster advancements in drug development and ultimate clinical applications.
(Click to Enlarge) Freshly isolated Rat Hippocampal Neurons (RHiN) cultured for 5 days (A). Freshly isolated RHiN (B), and revived cryopreserved RHiN (C) stained on day 8 for β III-Tubulin (green), GFAP (red), and DAPI (blue).
Details
| Tissue | Normal healthy rat hippocampus |
| QC | No bacteria, yeast, fungi, mycoplasma |
| Character | Positive for β-III Tubulin |
| Bioassay | Plate on Poly-D-Lysine coated surface, arborize to form neurite network in Culture Med |
Cryovial | 1M RHiN cryopreserved after isolation in Serum-Free Freezing Med (042-50) |
Fresh Culture | Contact CAI if interested in freshly isolated RHiN (suspension or plated) |
Plated | RHiN from cyrovial, shipped in Culture Medium |
Cryo Kit | RHiN (R886N-10), Cltr Med (R817-100) Ctng Soln I (027-05), Pltng Med (R886P-10) |
Suspn Kit | Frsh RHiN (R887N-10), Cultr Med (R817-100) Ctng Soln I (027-05), Pltng Med (R886P-10) |
Plated Kit | RHiN in multiwell plates, Cultr Med (R817-50) |
| Doublings | N/A - Neurons don't proliferate in vitro |
| Applications | Laboratory research use only (RUO). Not for human, clinical, diagnostic or veterinary use. |
Products
| Product | Size | CAT.# | Price | Quantity |
|---|---|---|---|---|
| Cryopreserved RHiN: Frozen RHiN (1x10^6) | Size: 1 Cryovial | CAT.#: R886N-10 | Price: $408.98 | |
| Cryopreserved RHiN Total Kit: 1x10^6 Cells, Plating Medium, Culture Medium & Coating Solution (See Details tab for specifics) | Size: 1 Kit | CAT.#: R886NK-10 | Price: $540.71 | |
| Freshly isolated RHiN: Contact CAI for details | Size: 1 Tube | CAT.#: R887NF-10 | Price: $408.98 | |
| Pre-plated freshly isolated RHiN: Contact CAI for details | Size: 6 Well | CAT.#: R887NF-6W | Price: $0.00 | |
| Pre-plated freshly isolated RHiN: Contact CAI for details | Size: 24 Well | CAT.#: R887NF-24W | Price: $0.00 | |
| Pre-plated freshly isolated RHiN: Contact CAI for details | Size: 96 Well | CAT.#: R887NF-96W | Price: $0.00 | |
| Pre-plated from cryopreserved RHiN: Plated RHiN from cryopreserved vials | Size: 6 Well | CAT.#: R887N-6W | Price: $538.13 | |
| Pre-plated from cryopreserved RHiN: Plated RHiN from cryopreserved vials | Size: 24 Well | CAT.#: R887N-24W | Price: $563.75 | |
| Pre-plated from cryopreserved RHiN: Plated RHiN from cryopreserved vials | Size: 96 Well | CAT.#: R887N-96W | Price: $604.75 |
Related Products
| Product | Size | CAT.# | Price | Quantity |
|---|---|---|---|---|
| Neuron Coating Solution I: Poly-D-Lysine coating for neurons | Size: 10 ml | CAT.#: 027-10 | Price: $30.75 | |
| Neuron Coating Solution I: Poly-D-Lysine coating for neurons | Size: 5 ml | CAT.#: 027-05 | Price: $16.14 | |
| Rat Hippocampal Neuron Plating Medium: Use for initial plating of RHiN. | Size: 10 ml | CAT.#: R886P-10 | Price: $20.45 | |
| Rat Neuron Basal Medium: Basal medium (contains no growth supplement). Add GS before use. | Size: 100 ml | CAT.#: R816-100 | Price: $31.21 | |
| Rat Neuron Culture Medium : For the culture and maintenance of neurons. | Size: 100 ml | CAT.#: R817-100 | Price: $63.50 | |
| Rat Neuron Culture Medium Kit: Basal medium & growth supplement sold together packaged separately | Size: Yields 100 ml | CAT.#: R817K-100 | Price: $72.11 | |
| Rat Neuron Culture Supplement: Added to Basal Medium to create Culture Medium | Size: 3 ml | CAT.#: R817-GS | Price: $57.04 |
Extended Family Products
| Product | Size | CAT.# | Price | Quantity |
|---|---|---|---|---|
| Cyto-X Colorimetric Cell Counting Reagent: 500 tests | Size: 1 Bottle | CAT.#: 028-01 | Price: $138.84 | |
| Cyto-X Colorimetric Cell Counting Reagent: 100 tests | Size: Sample | CAT.#: 028-S | Price: $35.88 | |
| Cytofect™ Neuron Transfection Kit: 100 x 24-Well Rxns | Size: 1 Kit | CAT.#: TF886K | Price: $403.59 | |
| Cytofect™ Neuron Transfection Kit: 25 x 24-Well Rxns | Size: 1 Sample Kit | CAT.#: TF886KS | Price: $53.76 | |
| Rat Neuron Culture Medium : For the culture and maintenance of neurons | Size: 150 ml | CAT.#: R817-150 | Price: $92.25 |
Resources/Documents
Publications
2017
(Publications)