Instructions RHiN
5 Important Cell Culture Rules
MSDS Cryopreserved Cells
Cell Apps Flyer Nervous System
Cell Apps Flyer Brain Cells
Cell Apps Poster Primary Cells
Cell Applications Inc Brochure

Description

Rat Hippocampal Neurons (RHiN) are derived from the hippocampi of normal embryonic rats by standardized methods. When cultured under the recommended conditions, RHiN arborize and form complex neurite network in one week. RHiN Stain positive for β III-Tubulin.
The hippocampus, a major brain component located under the cerebral cortex, consists of two hippocampi, one in each side of the brain.  Belonging to the limbic system, the hippocampus functions in spacial memory and navigation, and the consolidation of information from short- to long-term memory. Hippocampal neurons provide a model in vitro system for studying neurophysiology, neural plasticity and memory storage.  Damage, disease or oxygen starvation in this region is associated with Alzheimer's disease, encephalitis, epilepsy and amnesia. Examining hippocampal neurons in those disease conditions will allow a better understanding of brain physiology, disease mechanisms, pathology, and foster advancements in drug development and ultimate clinical applications.
(Click to Enlarge) Freshly isolated Rat Hippocampal Neurons (RHiN) cultured for 5 days (A). Freshly isolated RHiN (B), and revived cryopreserved RHiN (C) stained on day 8 for β III-Tubulin (green), GFAP (red), and DAPI (blue).

Details

TissueNormal healthy rat hippocampus 
QCNo bacteria, yeast, fungi, mycoplasma
CharacterPositive for β-III Tubulin
BioassayPlate on Poly-D-Lysine coated surface, arborize to form neurite network in Culture Med
Cryovial
1M RHiN cryopreserved after isolation in Serum-Free Freezing Med (042-50)
  
Cryo Kit
RHiN (R886N-10), Cltr Med (R817-100) Ctng Soln I (027-05), Pltng Med (R886P-10)
  
  
DoublingsN/A - Neurons don't proliferate in vitro
ApplicationsLaboratory research use only (RUO). Not for human, clinical, diagnostic or veterinary use.
Instructions RHiN

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MSDS Cryopreserved Cells

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Products

Product Size CAT.# Price Quantity
Cryopreserved RHiN : 1x10^6 Cells, Plating Medium, Culture Medium & Coating Solution (See Details tab for specifics) Size: 1 Kit CAT.#: R886NK-10 Price: $518.00
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Cryopreserved RHiN: Frozen RHiN (1x10^6) Size: 1 Cryovial CAT.#: R886N-10 Price: $429.00
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Related Products

Product Size CAT.# Price Quantity
Rat Neuron Culture Medium: For the culture and maintenance of neurons. Size: 100 ml CAT.#: R817-100 Price: $63.00
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Rat Neuron Culture Medium Kit: Basal medium & growth supplement sold together packaged separately Size: Yields 100 ml CAT.#: R817K-100 Price: $72.00
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Rat Neuron Basal Medium: Basal medium (contains no growth supplement).  Add GS before use. Size: 100 ml CAT.#: R816-100 Price: $31.00
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Rat Neuron Culture Supplement: Added to Basal Medium to create Culture Medium Size: 3 ml CAT.#: R817-GS Price: $57.00
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Neuron Coating Solution I: Poly-D-Lysine coating for neurons Size: 5 ml CAT.#: 027-05 Price: $16.00
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Neuron Coating Solution I: Poly-D-Lysine coating for neurons Size: 10 ml CAT.#: 027-10 Price: $31.00
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RHiN Plating Medium: Use for initial plating of RHiN. Size: 10 ml CAT.#: R886P-10 Price: $20.00
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Extended Family Products

Product Size CAT.# Price Quantity
Cytofect™ Neuron Transfection Kit: 100 x 24-Well Rxns Size: 1 Kit CAT.#: TF886K Price: $404.00
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Cytofect™ Neuron Transfection Kit: 25 x 24-Well Rxns Size: 1 Sample Kit CAT.#: TF886KS Price: $54.00
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Cyto-X Cell Counting Reagent: 500 tests Size: 1 Bottle CAT.#: 028-01 Price: $139.00
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Cyto-X Cell Counting Reagent Sample: 100 tests Size: Sample CAT.#: 028-S Price: $36.00
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Resources/Documents

5 Important Cell Culture Rules

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Cell Apps Flyer Nervous System

Format: PDF

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Cell Apps Flyer Brain Cells

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Cell Apps Poster Primary Cells

Format: PDF

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Cell Applications Inc Brochure

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Publications

2017

(Publications)