Instructions RDF
5 Important Cell Culture Rules
MSDS Cryopreserved Cells
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Description
Rat Dermal Fibroblasts (RDF) from Cell Applications, Inc. have been used in allogenic prosthetic grafts, demonstrating improved epithelization, collagen organization and faster regeneration. The cells were also used in a cell sheet engineering study, where a sheet of dermal fibroblasts was transplanted into the damaged liver in allogeneic rat model to promote migration of hepatic progenitor cells and faster healing, and as a control in engineered cardiac tissues.
(Click to Enlarge) Rat Dermal Fibroblasts: RDF. Isolated from rodent skin tissue.
Details
Tissue | Normal healthy rat dermis |
QC | No bacteria, yeast, fungi, mycoplasma |
Bioassay | Attach, spread, proliferate in Growth Med |
Cryovial | 500,000 RDF (1st passage) frozen in Basal Medium w/ 10% FBS, 10% DMSO |
Kit | Cryovial frozen RDF (R106-05n), Growth Medium (R116-500), Subcltr Rgnt Kit (090K) |
Proliferating | Shipped in Gr Med, psg 2 (flasks or plates) |
Doublings | At least 8 |
Applications | Laboratory research use only (RUO). Not for human, clinical, diagnostic or veterinary use. |
Products
| Product | Size | CAT.# | Price | Quantity |
|---|---|---|---|---|
| Cryopreserved Rat Dermal Fibroblast Total Kit, neonatal: 5x10^5 Cells (Neonatal), Medium & Subculture Reagents (See Details tab for specifics) | Size: 1 Kit | CAT.#: R106K-05n | Price: $602.00 | |
| Cryopreserved Rat Dermal Fibroblasts (RDF), neonatal: Frozen RDF (5x10^5) | Size: 1 Cryovial | CAT.#: R106-05n | Price: $435.00 | |
| Proliferating Rat Dermal Fibroblasts (RDF), neonatal: Actively growing, dividing cells in medium | Size: T-25 Flask | CAT.#: R107-25n | Price: $435.00 | |
| Proliferating Rat Dermal Fibroblasts (RDF), neonatal: Actively growing, dividing cells in medium | Size: T-75 Flask | CAT.#: R107-75n | Price: $625.00 | |
| Proliferating Rat Dermal Fibroblasts (RDF), neonatal: Actively growing, dividing cells in medium | Size: 24 Well | CAT.#: R107-24Wn | Price: $625.00 | |
| Proliferating Rat Dermal Fibroblasts (RDF), neonatal: Actively growing, dividing cells in medium | Size: 96 Well | CAT.#: R107-96Wn | Price: $745.00 | |
| Cryopreserved Rat Dermal Fibroblast Total Kit, adult: 5x10^5 Cells (Adult), Medium & Subculture Reagents (See Details tab for specifics) | Size: 1 Kit | CAT.#: R106K-05a | Price: $624.00 | |
| Cryopreserved Rat Dermal Fibroblasts (RDF), adult: Frozen RDF (5x10^5) | Size: 1 Cryovial | CAT.#: R106-05a | Price: $457.00 | |
| Proliferating Rat Dermal Fibroblasts (RDF), adult: Actively growing, dividing cells in medium | Size: T-25 Flask | CAT.#: R107-25a | Price: $457.00 | |
| Proliferating Rat Dermal Fibroblasts (RDF), adult: Actively growing, dividing cells in medium | Size: T-75 Flask | CAT.#: R107-75a | Price: $647.00 | |
| Proliferating Rat Dermal Fibroblasts (RDF), adult: Actively growing, dividing cells in medium | Size: 24 Well | CAT.#: R107-24Wa | Price: $647.00 | |
| Proliferating Rat Dermal Fibroblasts (RDF), adult: Actively growing, dividing cells in medium | Size: 96 Well | CAT.#: R107-96Wa | Price: $767.00 |
Related Products
| Product | Size | CAT.# | Price | Quantity |
|---|---|---|---|---|
| Rat Fibroblast Basal Medium: Basal medium (contains no growth supplement). Add GS before use. | Size: 500 ml | CAT.#: R115-500 | Price: $74.00 | |
| Rat Fibroblast Growth Medium: All-in-one ready-to-use media | Size: 500 ml | CAT.#: R116-500 | Price: $114.00 | |
| Rat Fibroblast Growth Medium Kit: Basal medium & growth supplement sold together packaged separately | Size: Yields 500 ml | CAT.#: R116K-500 | Price: $124.00 | |
| Rat Fibroblast Growth Supplement: Added to Basal Medium to create Growth Medium | Size: 50 ml | CAT.#: R116-GS | Price: $66.00 | |
| Rat Fibroblast Basal Medium wo Phenol Red: Basal medium (contains no growth supplement). Add GS before use. Without Phenol Red. | Size: 500 ml | CAT.#: R115PR-500 | Price: $86.00 | |
| Rat Fibroblast Growth Medium wo FBS: Growth Medium without FBS | Size: 500 ml | CAT.#: R116F-500 | Price: $131.00 | |
| Rat Fibroblast Growth Supplement wo FBS: Growth Supplement without FBS | Size: 50 ml | CAT.#: R116F-GS | Price: $75.00 | |
| Rat Fibroblast Growth Medium wo Phenol Red: All-in-one ready-to-use media, without Phenol Red | Size: 500 ml | CAT.#: R116PR-500 | Price: $131.00 |
Extended Family Products
| Product | Size | CAT.# | Price | Quantity |
|---|---|---|---|---|
| Cyto-X Cell Counting Reagent: 500 tests | Size: 1 Bottle | CAT.#: 028-01 | Price: $160.00 | |
| Cyto-X Cell Counting Reagent Sample: 100 tests | Size: Sample | CAT.#: 028-S | Price: $41.00 | |
| Cytofect Fibroblast Transfection Kit (250 x 24-Wells): 250 x 24-Well Rxns | Size: 1 Kit | CAT.#: TF103K | Price: $465.00 | |
| Cytofect Fibroblast Transfection Sample Kit (25 x 24-Wells): 25 x 24-Well Rxns | Size: 1 Sample Kit | CAT.#: TF103KS | Price: $62.00 | |
| Subculture Reagent Kit: 100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution | Size: 1 Kit | CAT.#: 090K | Price: $63.00 | |
| Rat Fibroblast Growth Factor-9 (FGF-9): Rat Fibroblast Growth Factor-9 | Size: 10 ug | CAT.#: RP3027-10 | Price: $194.00 | |
| Rat Fibroblast Growth Factor-9 (FGF-9): Rat Fibroblast Growth Factor-9 | Size: 100 ug | CAT.#: RP3027-100 | Price: $484.00 | |
| Rat Fibroblast Growth Factor-9 (FGF-9): Rat Fibroblast Growth Factor-9 | Size: 1000 ug | CAT.#: RP3027-1000 | Price: $4,090.00 |
Resources/Documents
Publications
2017
Uchinaka A, Kawaguchi N, Ban T, Hamada Y, Mori S, Maeno Y, Sawa Y, Nagata K, Yamamoto H. 2017. Evaluation of dermal wound healing activity of synthetic peptide SVVYGLR. Biochem Biophys Res Commun. 2017 Jul 24. pii: S0006-291X(17)31482-1.
Fuji, M., K. Yamanouchi, Y. Saka, Z. Baimakhanov, I. Yamaguchi, A. Soyam, M. Hidka, M. Takatsuki, T. Kurokiand S. Eguchi. 2017. In vivo construction of liver tissue by implantation of a hepatic non-parenchymal/adipose-derived stem cell sheet. J. Tiss Eng & Regen Med, DOI:10.1002/term.2424.
2016
Rathinasabapathy, A., E. Bruce, A. Espejo, A. Horowitz, D. Sudhan, A. Nair, D. Guzzo, J. Francis, M. Raizada, V. Shenoy and M. Katovich. 2016. Therapeutic potential of adipose stem cell-derived conditioned medium against pulmonary hypertension and lung fibrosis. British J Pharmacol, 173:2859-2879.
2015
Aalbers, C., L. Bevaart, S. Loiler, K. de Cortie, J. Wright, F. Mingozzi, P. Tak and M. Vervoordeldonk. 2015. Preclinical Potency and Biodistribution Studies of an AAV 5 Vector Expressing Human Interferon-β (ART-I02) for Local Treatment of Patients with Rheumatoid Arthritis. PLoS ONE 10(6): e0130612.
Hiroharu, O., H. Ryusuke, O. Satoshi, F. Yuki, L. Xing, T. Michiaki, K. Mika and K. Yukinari. 2015. Development of Sensitive Monoclonal Antibody PMab-2 Against Rat Podoplanin. mAbs in Immunodiag & Immunother, 34:396-403. RDF
Uchinaka, A., Y. Hamada, S. Mori, S. Miyagawa, A. Saito, Y. Sawa, N. Matsuura, H. Yamamoto and N. Kawaguchi. 2015. SVVYGLR motif of the thrombin-cleaved N-terminal osteopontin fragment enhances the synthesis of collagen type III in myocardial fibrosis.Molec & Cell Biochem, 408:191-203.
2014
Fei, R., H. Hou, D. Munoz-Pinto, A. Han, M. Hahn, and M. Grunlan. 2014. Thermoresponsive Double Network Micropillared Hydrogels for Controlled Cell Release. Macromolecular Bioscience, 14:1346-1352.
Yan, P., X. Min, Z. Jiping, L. Hongwei, Z. Ziyuan, W. Yu and C. Biao. 2014. Freeze-Dried Rat Bone Marrow Mesenchymal Stem Cell Paracrine Factors: A Simplified Novel Material for Skin Wound Therapy. Tissue Eng Pt A, 21:1036-1046.
2013
Muraoka, I., M. Takatsuki, Y. Sakai, T. Tomonaga, A. Soyama, M. Hidaka, Y. Hishikawa, T. Koji, R. Utoh, and K. Ohashi. 2013. Transplanted fibroblast cell sheets promote migration of hepatic progenitor cells in the incised host liver in allogeneic rat model. Journal of tissue engineering and regenerative medicine. doi: 10.1002/term.1718.
2012
Nomoto, Y., W. Okano, M. Imaizumi, A. Tani, M. Nomoto, and K. Omori. 2012. Bioengineered prosthesis with allogenic heterotopic fibroblasts for cricoid regeneration. The Laryngoscope. 122:805-809.
Serrao, G.W., I.C. Turnbull, D. Ancukiewicz, D.E. Kim, E. Kao, T.J. Cashman, L. Hadri, R.J. Hajjar, and K.D. Costa. 2012. Myocyte-depleted engineered cardiac tissues support therapeutic potential of mesenchymal stem cells. Tissue Engineering Part A. 18:1322-1333.