Instructions RCoN R822N-20
Instructions RCoN R822N-40
5 Important Cell Culture Rules
MSDS Cryopreserved Cells
Cell Apps Flyer Nervous System
Cell Apps Flyer Brain Cells
Cell Apps Poster Primary Cells
Brochure Cell Applications Inc

Description

Rat cortical neurons (RCoN) are derived from cerebral cortices of day 18 embryonic Sprague Dawley rat brains. When cultured under the recommended conditions, RCoN arborize and form complex neurite network in one week. RCoN Stain positive for β III-Tubulin.  Rat cortical neurons are available in multiple formats, including cryopreserved neurons, freshly isolated primary neurons in suspension, or pre-plated cultured neurons.
The cerebral cortex plays a key role in cognition, memory, attention, perceptual awareness, thought, language and consciousness. Neurons connect to other neural cells through neurites and synapses to form complex neurocircuitry for signal transmission. A healthy circuitry in the brain is essential for cognitive functions, proper control and regulation of the other parts of the body. Dysfunction and degeneration in the cerebral cortex have been implicated in various brain injuries, neurological and psychiatric disorders. Cortical neurons, therefore, provide an excellent cellular model system to study disease mechanisms and pathophysiologies.  They also serve as a platform for drug screening and development, toxicity tests, immunostaining, live cell imaging, co-culturing and electrophysiology.
(Click to Enlarge) Freshly isolated Rat Cortical Neurons (RCoN) cultured for 5 days (A). Freshly isolated RCoN (B), and revived cryopreserved RCoN (C) stained on day 8 for β III-Tubulin (green), GFAP (red), and DAPI (blue).

Details

TissueNormal healthy rat  brain cortex   
QCNo bacteria, yeast, fungi, mycoplasma
CharacterPositive for β-III Tubulin
BioassayPlate on Poly-D-Lysine coated surface, arborize to form neurite network in Culture Med
Cryovial2 or 4M RCoN cryopreserved after isolation in Serum-Free Freezing Medium (042-50)
Kit (2M)2M cryopreserved RCoN (R882N-20), Coating Soln I (027-05), Plating Med (R817P-20), Culture Med (R817-100)
Kit (4M)4M cryopreserved RCoN (R882N-40), Coating Soln I (027-10), Plating Med (R817P-40), Culture Med (R817-150)
Fresh CultureContact CAI if interested in freshly isolated RCoN (suspension or plated)
Plated2M RCoN from cryovial, in Culture Medium
DoublingsN/A - Neurons don't proliferate in vitro
ApplicationsLaboratory research use only (RUO). Not for human, clinical, diagnostic or veterinary use.
Instructions RCoN R822N-20

Format: PDF

Download Now
Instructions RCoN R822N-40

Format: PDF

Download Now
MSDS Cryopreserved Cells

Format: PDF

Download Now

Products

Product Size CAT.# Price Quantity
Cryopreserved RCoN : 4x10^6 Cells, Plating Medium, Culture Medium & Coating Solution (See Details tab for specifics) Size: 1 Kit CAT.#: R882NK-40 Price: $610.00
-+
Cryopreserved RCoN: Frozen RCoN (2x10^6) Size: 1 Cryovial CAT.#: R882N-20 Price: $282.00
-+
Cryopreserved RCoN: Frozen RCoN (4x10^6) Size: 1 Cryovial CAT.#: R882N-40 Price: $502.00
-+
Cryopreserved RCoN : 2x10^6 Cells, Plating Medium, Culture Medium & Coating Solution (See Details tab for specifics) Size: 1 Kit CAT.#: R882NK-20 Price: $390.00
-+
Freshly Isolated RCoN: Contact CAI for details or request a quote Size: 1 Tube CAT.#: R883NF-20 Price: $0.00
-+
Pre-plated Freshly Isolated RCoN: Contact CAI for details or request a quote Size: 24 Well CAT.#: R883NF-24W Price: $0.00
-+
Pre-plated Freshly Isolated RCoN: Contact CAI for details or request a quote Size: 96 Well CAT.#: R883NF-96W Price: $0.00
-+
Pre-plated Freshly Isolated RCoN: Contact CAI for details or request a quote Size: 6 Well CAT.#: R883NF-6W Price: $0.00
-+
Pre-plated from Cryopreserved RCoN: Plated RCoN from cryopreserved vials Size: 6 Well CAT.#: R883N-6W Price: $384.00
-+
Pre-plated from Cryopreserved RCoN: Plated RCoN from cryopreserved vials Size: 24 Well CAT.#: R883N-24W Price: $400.00
-+
Pre-plated from Cryopreserved RCoN: Plated RCoN from cryopreserved vials Size: 96 Well CAT.#: R883N-96W Price: $436.00
-+

Related Products

Product Size CAT.# Price Quantity
Neuron Coating Solution I: Poly-D-Lysine coating for neurons Size: 10 ml CAT.#: 027-10 Price: $31.00
-+
Neuron Coating Solution I: Poly-D-Lysine coating for neurons Size: 5 ml CAT.#: 027-05 Price: $16.00
-+
Rat Neuron Culture Medium: For the culture and maintenance of neurons Size: 150 ml CAT.#: R817-150 Price: $92.00
-+
Rat Neuron Culture Medium: For the culture and maintenance of neurons. Size: 100 ml CAT.#: R817-100 Price: $63.00
-+
Rat Neuron Culture Medium Kit: Basal medium & growth supplement sold together packaged separately Size: Yields 100 ml CAT.#: R817K-100 Price: $72.00
-+
Rat Neuron Culture Supplement: Added to Basal Medium to create Culture Medium Size: 3 ml CAT.#: R817-GS Price: $57.00
-+
Rat Neuron Plating Solution I: For initial plating of neurons Size: 20 ml CAT.#: R817P-20 Price: $39.00
-+
Rat Neuron Plating Solution I: For initial plating of neurons Size: 40 ml CAT.#: R817P-40 Price: $62.00
-+

Extended Family Products

Product Size CAT.# Price Quantity
Cyto-X Cell Counting Reagent: 500 tests Size: 1 Bottle CAT.#: 028-01 Price: $139.00
-+
Cyto-X Cell Counting Reagent Sample: 100 tests Size: Sample CAT.#: 028-S Price: $36.00
-+
Cytofect™ Neuron Transfection Kit: 100 x 24-Well Rxns Size: 1 Kit CAT.#: TF886K Price: $404.00
-+
Cytofect™ Neuron Transfection Kit: 25 x 24-Well Rxns Size: 1 Sample Kit CAT.#: TF886KS Price: $54.00
-+
Rat Neuron Culture Medium: For the culture and maintenance of neurons Size: 150 ml CAT.#: R817-150 Price: $92.00
-+

Resources/Documents

5 Important Cell Culture Rules

Format: PDF

Downoad Now
Cell Apps Flyer Nervous System

Format: PDF

Downoad Now
Cell Apps Flyer Brain Cells

Format: PDF

Downoad Now
Cell Apps Poster Primary Cells

Format: PDF

Downoad Now