Instructions RCF
5 Important Cell Culture Rules
MSDS Cryopreserved Cells
Cell Apps Flyer Cardiovascular Cells
Cell Apps Poster Primary Cells
Cell Applications Inc Brochure

Description

Rat Cardiac Fibroblasts (RCF) are the most prevalent cell type in the rodent's heart, making up 60-70 % of all cells.  They are known to play key roles in the development of cardiac disease and hypertrophy.  Rat Cardiac Fibroblasts from Cell Applications, Inc. provide an excellent model system to study many aspects of heart function and pathophysiology, including the regulation of cell growth, survival, inflammation, hormone secretion and metabolism.  
(Click to Enlarge) Rat Cardiac Fibroblasts: RCF.  Isolated from rodent heart tissue.

Details

Tissue
Normal healthy rat heart
QC
No bacteria, yeast, fungi, mycoplasma
Bioassay
Attach, spread, proliferate in Growth Med
Cryovial
500,000 RCF (2nd passage) in frozen in Basal Medium w/ 10% FBS, 10% DMSO
Kit
Cryovial frozen RCF (R306-05a/-05n), Gr Med (R116-500), Subcltr Rgnt Kit (090K)
Proliferating
Shipped within 3 days after plating in Cltr Medium (flasks or plates)
Doublings
At least 8
Applications
Laboratory research use only (RUO). Not for human, clinical, diagnostic or veterinary use.
Instructions RCF

Format: PDF

Download Now
MSDS Cryopreserved Cells

Format: PDF

Download Now

Products

Product Size CAT.# Price Quantity
Cryopreserved RCF, Adult: Frozen RCF (5x10^5) Size: 1 Cryovial CAT.#: R306-05a Price: $640.00
-+
Cryopreserved RCF, Adult: 5x10^5 Cells (Adult), Medium & Subculture Reagents (See Details tab for specifics) Size: 1 Kit CAT.#: R306K-05a Price: $784.00
-+
Cryopreserved RCF, Fetal: Frozen RCF (5x10^5) Size: 1 Cryovial CAT.#: R306-05f Price: $662.00
-+
Cryopreserved RCF, Fetal: 5x10^5 Cells (Fetal), Medium & Subculture Reagents (See Details tab for specifics) Size: 1 Kit CAT.#: R306K-05f Price: $806.00
-+
Cryopreserved RCF, Neonatal: 5x10^5 Cells (Neonatal), Medium & Subculture Reagents (See Details tab for specifics) Size: 1 Kit CAT.#: R306K-05n Price: $784.00
-+
Cryopreserved RCF, Neonatal: Frozen RCF (5x10^5) Size: 1 Cryovial CAT.#: R306-05n Price: $640.00
-+
Proliferating RCF, Adult: Actively growing, dividing cells in medium Size: T-25 Flask CAT.#: R307-25a Price: $640.00
-+
Proliferating RCF, Adult: Actively growing, dividing cells in medium Size: T-75 Flask CAT.#: R307-75a Price: $845.00
-+
Proliferating RCF, Adult: Actively growing, dividing cells in medium Size: 24 Well CAT.#: R307-24Wa Price: $845.00
-+
Proliferating RCF, Adult: Actively growing, dividing cells in medium Size: 96 Well CAT.#: R307-96Wa Price: $974.00
-+
Proliferating RCF, Fetal: Actively growing, dividing cells in medium Size: 96 Well CAT.#: R307-96Wf Price: $985.00
-+
Proliferating RCF, Fetal: Actively growing, dividing cells in medium Size: T-25 Flask CAT.#: R307-25f Price: $662.00
-+
Proliferating RCF, Fetal: Actively growing, dividing cells in medium Size: 24 Well CAT.#: R307-24Wf Price: $856.00
-+
Proliferating RCF, Fetal: Actively growing, dividing cells in medium Size: T-75 Flask CAT.#: R307-75f Price: $856.00
-+
Proliferating RCF, Neonatal: Actively growing, dividing cells in medium Size: T-25 Flask CAT.#: R307-25n Price: $640.00
-+
Proliferating RCF, Neonatal: Actively growing, dividing cells in medium Size: T-75 Flask CAT.#: R307-75n Price: $845.00
-+
Proliferating RCF, Neonatal: Actively growing, dividing cells in medium Size: 6 Well CAT.#: R307-6Wn Price: $845.00
-+
Proliferating RCF, Neonatal: Actively growing, dividing cells in medium Size: 96 Well CAT.#: R307-96Wn Price: $974.00
-+

Related Products

Product Size CAT.# Price Quantity
Rat Fibroblast Basal Medium: Basal medium (contains no growth supplement).  Add GS before use. Size: 500 ml CAT.#: R115-500 Price: $57.00
-+
Rat Fibroblast Basal Medium wo Phenol Red: Basal medium (contains no growth supplement). Add GS before use. Without Phenol Red. Size: 500 ml CAT.#: R115PR-500 Price: $66.00
-+
Rat Fibroblast Growth Medium: All-in-one ready-to-use media Size: 500 ml CAT.#: R116-500 Price: $99.00
-+
Rat Fibroblast Growth Medium Kit: Basal medium & growth supplement sold together packaged separately Size: Yields 500 ml CAT.#: R116K-500 Price: $108.00
-+
Rat Fibroblast Growth Medium wo FBS: Growth Medium without FBS Size: 500 ml CAT.#: R116F-500 Price: $114.00
-+
Rat Fibroblast Growth Medium wo Phenol Red: All-in-one ready-to-use media, without Phenol Red Size: 500 ml CAT.#: R116PR-500 Price: $114.00
-+
Rat Fibroblast Growth Supplement: Added to Basal Medium to create Growth Medium Size: 50 ml CAT.#: R116-GS Price: $51.00
-+
Rat Fibroblast Growth Supplement wo FBS: Growth Supplement without FBS Size: 50 ml CAT.#: R116F-GS Price: $58.00
-+

Extended Family Products

Product Size CAT.# Price Quantity
Cyto-X Cell Counting Reagent: 500 tests Size: 1 Bottle CAT.#: 028-01 Price: $139.00
-+
Cyto-X Cell Counting Reagent Sample: 100 tests Size: Sample CAT.#: 028-S Price: $36.00
-+
Cytofect™ Fibroblast Transfection Kit: 250 x 24-Well Rxns Size: 1 Kit CAT.#: TF103K Price: $404.00
-+
Cytofect™ Fibroblast Transfection Kit: 25 x 24-Well Rxns Size: 1 Sample Kit CAT.#: TF103KS Price: $54.00
-+
Rat Fibroblast Growth Factor-9 (FGF-9): Rat Fibroblast Growth Factor-9 Size: 10 ug CAT.#: RP3027-10 Price: $194.00
-+
Rat Fibroblast Growth Factor-9 (FGF-9): Rat Fibroblast Growth Factor-9 Size: 100 ug CAT.#: RP3027-100 Price: $484.00
-+
Rat Fibroblast Growth Factor-9 (FGF-9): Rat Fibroblast Growth Factor-9 Size: 1000 ug CAT.#: RP3027-1000 Price: $4,090.00
-+
RCF RNA, Adult : Total RNA prepared from Rat Cardiac Fibroblasts Size: 10 ug CAT.#: R306-R10a Price: $350.00
-+
RCF RNA, Adult : Total RNA prepared from Rat Cardiac Fibroblasts Size: 25 ug CAT.#: R306-R25a Price: $700.00
-+
Subculture Reagent Kit: 100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution Size: 1 Kit CAT.#: 090K Price: $55.00
-+

Resources/Documents

5 Important Cell Culture Rules

Format: PDF

Downoad Now
Cell Apps Flyer Cardiovascular Cells

Format: PDF

Downoad Now
Cell Apps Poster Primary Cells

Format: PDF

Downoad Now
Cell Applications Inc Brochure

Format: PDF

Downoad Now

Publications

2015
Nakayama, A., H. Morita, T. Nakao, T. Yamaguchi, T. Sumida, Y. Ikeda, H. Kumagai, Y. Motozawa, T. Takahashi, A. Imaizumi, T. Hashimoto, R. Nagai, and I. Komuro. 2015. A Food-Derived Flavonoid Luteolin Protects against Angiotensin II-Induced Cardiac Remodeling. PLOS One,    DOI: 10.1371/journal.pone.0137106.
2011
Hao, K., H. Hanawa, L. Ding, Y. Ota, K. Yoshida, K. Toba, M. Ogura, H. Ito, M. Kodama, and Y. Aizawa. 2011. Free heme is a danger signal inducing expression of proinflammatory proteins in cultured cells derived from normal rat hearts. Molec Immunol, 48:1191-1202.
Silberbach, M., C. Roberts, and N. Airhart. 2011. Methods of screening using a natriuretic peptide receptor. Patent US 7943296 B2.
Silberbach, M., C. Roberts, and N. Airhart. 2011. Natriuretic peptide related fragment in cardiovascular disease. Patent Application US 20110218230 A1.