Instructions HUVSMC
5 Important Cell Culture Rules
MSDS Cryopreserved Cells
Cell Apps Flyer Smooth Muscle Cells
Cell Apps Flyer Cardiovascular Cells
Cell Apps Poster Primary Cells
Cell Applications Inc Brochure
Description
Human Umbilical Vein Smooth Muscle Cells (HUVSMC) from Cell Applications, Inc. have been used in studies evaluating treatments that involve blood vessel stents.
- Specifically, intra-shunt delivery of motexafin gadolinium was shown to inhibit the proliferation of SMCs during treatment of portal hypertension
- Also, high-density lipoproteins (HDL) exhibit cardioprotective and anti-inflammatory properties by reducing HUVSMC expression of chemokines CCL2, CCL5, and CX3CL1, and chemokine receptors CCR2 and CX3CR1 (via NF-κB/pAkt inhibition) and by decreasing cell proliferation (via pERK inhibition), highlighting HDL importance in preventing neointimal hyperplasia, the main cause of early vein graft/stent failure
- HUVSMC were also used to demonstrate that genes coding for muscle and non-muscle actin proteins are transcribed by different transcription machinery
Characterization: positive for smooth muscle cell specific alpha-actin expression
(Click to Enlarge) Human Umbilical Vein Smooth Muscle Cells: HUVSMC. Cells isolated from the vessel that carries oxygenated blood from the placenta to the fetus.
Details
Tissue | Normal healthy human umbilical vein |
QC | No bacteria, yeast, fungi, mycoplasma, virus |
Character | Smooth muscle specific α-actin positive |
Bioassay | Attach, spread, proliferate in Growth Med |
Cryovial | 500,000 HUVSMC (2nd passage) frozen in Basal Medium w/ 10% FBS, 10% DMSO |
Kit | Cryovial frozen HUVSMC (250-05n), Growth Medium (311-500), Subcltr Rgnt Kit (090K) |
Proliferating | Shipped in Gr Med, 3rd psg (flasks or plates) |
Doublings | At least 6 |
Applications | Laboratory research use only (RUO). Not for human, clinical, diagnostic or veterinary use. |
Products
| Product | Size | CAT.# | Price | Quantity |
|---|---|---|---|---|
| Cryopreserved Human Umbilical Vein Smooth Muscle Cell Total Kit, neonatal: 5x10^5 Cells (Adult), Medium & Subculture Reagents (See Details tab for specifics) | Size: 1 Kit | CAT.#: 250K-05n | Price: $707.00 | |
| Cryopreserved Umbilical Vein Smooth Muscle Cells (HUVSMC), neonatal: Frozen HUVSMC (5x10^5) | Size: 1 Cryovial | CAT.#: 250-05n | Price: $533.00 | |
| Proliferating Umbilical Vein Smooth Muscle Cells (HUVSMC), neonatal: Actively growing, dividing cells in medium | Size: T-25 Flask | CAT.#: 251-25n | Price: $533.00 | |
| Proliferating Umbilical Vein Smooth Muscle Cells (HUVSMC), neonatal: Actively growing, dividing cells in medium | Size: T-75 Flask | CAT.#: 251-75n | Price: $723.00 | |
| Proliferating Umbilical Vein Smooth Muscle Cells (HUVSMC), neonatal: Actively growing, dividing cells in medium | Size: 24 Well | CAT.#: 251-24Wn | Price: $723.00 | |
| Proliferating Umbilical Vein Smooth Muscle Cells (HUVSMC), neonatal: Actively growing, dividing cells in medium | Size: 96 Well | CAT.#: 251-96Wn | Price: $843.00 |
Related Products
| Product | Size | CAT.# | Price | Quantity |
|---|---|---|---|---|
| Human SMC Basal Medium: Basal medium (contains no growth supplement). Add GS before use. | Size: 500 ml | CAT.#: 310-500 | Price: $74.00 | |
| Human SMC Differentiation Medium: Promotes cells to change from one type to another, more specialized | Size: 250 ml | CAT.#: 311D-250 | Price: $84.00 | |
| Human SMC Growth Medium: All-in-one ready-to-use | Size: 500 ml | CAT.#: 311-500 | Price: $121.00 | |
| Human SMC Growth Medium Kit: Basal medium & growth supplement sold together packaged separately | Size: Yields 500ml | CAT.#: 311K-500 | Price: $130.00 | |
| Human SMC Growth Supplement: Added to Basal Medium to create Growth Medium | Size: 30 ml | CAT.#: 311-GS | Price: $74.00 |
Extended Family Products
| Product | Size | CAT.# | Price | Quantity |
|---|---|---|---|---|
| Mouse Interleukin-6 Antibody: Mouse Interleukin-6 Antibody | Size: 100 ul | CAT.#: CP10325 | Price: $302.00 | |
| Freezing Medium: For general cryopreservation of most primary cells. Contains FBS & DMSO. | Size: 50 ml | CAT.#: 040-50 | Price: $54.00 | |
| Cyto-X Cell Counting Reagent: 500 tests | Size: 1 Bottle | CAT.#: 028-01 | Price: $160.00 | |
| Cyto-X Cell Counting Reagent Sample: 100 tests | Size: Sample | CAT.#: 028-S | Price: $41.00 | |
| Cytofect Smooth Muscle Cell Transfection Kit (125 x 24-Wells): 125 x 24-Well | Size: 1 Kit | CAT.#: TF350K | Price: $328.00 | |
| Cytofect Smooth Muscle Cell Transfection Sample Kit (25 x 24-Wells): 25 x 24-Well Rxns | Size: 1 Sample Kit | CAT.#: TF350KS | Price: $62.00 | |
| Umbilical Vein Smooth Muscle Cell RNA (HUVSMC RNA), Neonatal: Total RNA prepared from Human Umbilical Vein Smooth Muscle Cells, neonatal | Size: 10 ug | CAT.#: 250-R10n | Price: $465.00 | |
| Umbilical Vein Smooth Muscle Cell RNA (HUVSMC RNA), Neonatal: Total RNA prepared from Human Umbilical Vein Smooth Muscle Cells, neonatal | Size: 25 ug | CAT.#: 250-R25n | Price: $930.00 | |
| Human Interleukin-6 (IL-6): Human Interleukin-6 | Size: 20 ug | CAT.#: RP1010-20 | Price: $194.00 | |
| Human Interleukin-6 (IL-6): Human Interleukin-6 | Size: 100 ug | CAT.#: RP1010-100 | Price: $484.00 | |
| Human Interleukin-6 (IL-6): Human Interleukin-6 | Size: 1000 ug | CAT.#: RP1010-1000 | Price: $3,175.00 | |
| Human IL-6 ELISA Kit: Human Interleukin-6 ELISA Kit | Size: 96 Wells | CAT.#: CL0410 | Price: $517.00 | |
| Subculture Reagent Kit: 100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution | Size: 1 Kit | CAT.#: 090K | Price: $63.00 | |
| Human IL-6, Animal Free: Human Interleukin-6, Animal-Free | Size: 20 ug | CAT.#: RP1010AF-20 | Price: $213.00 | |
| Human IL-6, Animal Free: Human Interleukin-6, Animal-Free | Size: 100 ug | CAT.#: RP1010AF-100 | Price: $533.00 | |
| Human IL-6, Animal Free: Human Interleukin-6, Animal-Free | Size: 1000 ug | CAT.#: RP1010AF-1000 | Price: $3,492.00 |
Resources/Documents
Publications
2017
Vanags, L.Z., Tan, J.T., Santos, M., Michael, P.S., Ali, Z., Bilek, M.M., Wise, S.G. and Bursill, C.A., 2017. Plasma activated coating immobilizes apolipoprotein AI to stainless steel surfaces in its bioactive form and enhances biocompatibility. Nanomedicine: Nanotechnology, Biology and Medicine. DOI: 10.1016/j.nano.2017.06.012
2015
Gaston, B., A. Straub, B. Isakson, and L. Columbus. 2015. Compositions and Methods for Regulating Arterial Tone. Patent Application US 20150274779 A1.
2014
Thakali, K., J. Saben, J. Faske, F. Lindsey, H. Gomez-Acevedo, C. Lowery, T. Badger A. Andres and K. Shankar. 2014. Maternal pregravid obesity changes gene expression profiles toward greater inflammation and reduced insulin sensitivity in umbilical cord. Pediatric Res, 76:202-210.
2013
van der Vorst, E.P.C., L.Z. Vanags, L.L. Dunn, H.C. Prosser, K.-A. Rye, and C.A. Bursill. 2013. High-density lipoproteins suppress chemokine expression and proliferation in human vascular smooth muscle cells. The FASEB Journal. 27:1413-1425.
Wang, H., F. Zhang, Y. Meng, T. Zhang, P. Willis, T. Le, S. Soriano, E. Ray, K. Valji, G. Zhang, and X. Yang. 2013. MRI-Monitored Intra-Shunt Local Agent Delivery of Motexafin Gadolinium: Towards Improving Long-Term Patency of TIPS. PloS one. 8:e57419.
2011
Lee, Y.-L., C.-H. Chiao, and M.-T. Hsu. 2011. Transcription of Muscle Actin Genes by a Nuclear Form of Mitochondrial RNA Polymerase. PloS one. 6:e22583.
2010
Gelissen, I.C., S. Cartland, A.J. Brown, C. Sandoval, M. Kim, D.L. Dinnes, Y. Lee, V. Hsieh, K. Gaus, L. Kritharides, and W. Jessup. 2010. Expression and stability of two isoforms of ABCG1 in human vascular cells. Atherosclerosis. 208:75-82.
Maier, K., X. Han, B. Sadowitz, K. Gentile, F. Middleton and V. Gahtan. 2010. Thrombospondin-1: A proatherosclerotic protein augmented by hyperglycemia. J Vasc Surg, 51:1238-1247.
2007
Du, W., K.M. Pryse, J.A. Fee, E.L. Elson, and R.J. Okamoto. 2007. Vascular Smooth Muscle Cell Mechanics During Cyclic Stretch: Effect of Serum and a Serum Substitute. In ASME 2007 Summer Bioengineering Conference. American Society of Mechanical Engineers. 267-268.